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AB107921

Proteasome Activity Assay Kit

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(2 Reviews)

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(86 Publications)

Proteasome Activity Assay Kit ab107921 is based on a Succ-LLVY-AMC substrate which is cleaved by proteasome activity, releasing fluorescent AMC. Readout on any fluorometric (Ex/Em 350/440 nm) plate reader.

- Includes proteasome inhibitor control, proteasome positive control, and standard to allow proteasome activity quantitation
- Cited in > 80 publications
- Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
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Functional Studies - Proteasome Activity Assay Kit (AB107921)
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Functional Studies - Proteasome Activity Assay Kit (AB107921)

Proteasome Activity measured in HeLa lysate

Functional Studies - Proteasome Activity Assay Kit (AB107921)
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Functional Studies - Proteasome Activity Assay Kit (AB107921)

Standard curve : mean of duplicates (+/- SD) with background reads subtracted

Functional Studies - Proteasome Activity Assay Kit (AB107921)
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Functional Studies - Proteasome Activity Assay Kit (AB107921)

Proteasome Activity measured in Jurkat lysate

Functional Studies - Proteasome Activity Assay Kit (AB107921)
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Lab

Functional Studies - Proteasome Activity Assay Kit (AB107921)

Proteasome Activity measured in cell lysates showing activity (mU) per 1 mln cells.

Samples with the concentration of 3.3e6 cells/mL were used. 50 μl of each sample was used.

Key facts

Detection method

Fluorescent

Sample types

Cell Lysate

Assay type

Enzyme activity

Assay time

1h

Assay Platform

Microplate reader

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Enzyme activity assay": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Proteasome Activity Assay Kit ab107921 is a simple and reliable assay to quantify proteasome activity.

Proteasome activity assay principle
The 20S proteasome assembly is the functional protease structure in the proteasome, with chymotrypsin-like, trypsin-like and caspase-like protease activities. The assay is based on measuring the chymotrypsin-like activity.

The assay uses an AMC-tagged peptide substrate (Proteasome Substrate (Succ-LLVY-AMC in DMSO), which releases free, fluorescent AMC (Ex/Em 350/440 nm) in the presence of proteolytic activity.

The kit also includes a positive control (Jurkat cell lysate with significant proteasome activity), and a specific proteasome inhibitor MG-132 which suppresses all proteolytic activity due to proteasomes. This permits differentiation of proteasome activity from other protease activity which may be present in samples.

Proteasome activity assay protocol summary
- add samples, standards and positive control to wells
- add proteasome inhibitor to half of sample and positive control wells
- add proteasome substrate to sample and control wells
- incubate for 20 min
- analyze with microplate reader, incubate for further 30 min, analyze again

How other researchers are using Proteasome Activity Assay Kit ab107921
ab107921 has been used in a variety of sample types including:
- Mouse ovarian surface epithelial cell lines (1)
- Mouse intestine samples (2)
- Mouse brain lysates (3)
References: 1-Tailor D et al. 2023; 2-Hurley M et al. 2023; 3-Vulin M et al. 2023

Related and recommended productsProteasome 20S Activity Assay Kit (Fluorometric) ab112154 uses an alternative fluorophore and a similar principle to this assay, with an LLVY proteasome substrate.

This product was previously called K245 Biovision Proteasome Activity Fluorometric Assay Kit. Biovision was acquired by Abcam in 2021.

The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.

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What's included?

{ "values": { "100Test": { "sellingSize": "100 Test", "publicAssetCode":"ab107921-100Test", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"Proteasome Assay Buffer", "number":"AB107921-CMP05", "productcode":"" }, { "size":"1 x 100 µL", "name":"AMC Standard", "number":"AB107921-CMP01", "productcode":"" }, { "size":"1 x 100 µL", "name":"Proteasome Inhibitor", "number":"AB107921-CMP02", "productcode":"" }, { "size":"1 x 100 µL", "name":"Proteasome Substrate", "number":"AB107921-CMP04", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Proteasome Positive Control", "number":"AB107921-CMP03", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Proteasomes are large protein complexes responsible for degrading ubiquitinated proteins into peptides. This process is essential for maintaining protein homeostasis within the cell. The proteasome often referred to as the 26S proteasome consists of a core particle with a mass of roughly 700 kDa and a regulatory particle. They are expressed in the cytoplasm and nucleus of eukaryotic cells where they perform the bulk of cellular protein degradation. Other names for the proteasome include multicatalytic proteinase complex and prosome.
Biological function summary

The ubiquitin-proteasome system orchestrates the controlled degradation of unneeded or damaged proteins regulating numerous cellular mechanisms. The proteasome forms a complex with ubiquitin chains tagged to substrate proteins guiding them for degradation. Proteasomal activity ensures turnover of proteins involved in cell cycle progression signal transduction and immune responses. By controlling protein levels proteasome function influences many biological outcomes and maintains cellular equilibrium.

Pathways

The proteasome's function integrates deeply into the ubiquitin-proteasome pathway critical for protein catabolism and cellular control processes. Another significant pathway is the regulation of apoptosis where the proteasome controls the levels of proteins like p53 an important regulator of cell death. In these pathways the proteasome collaborates with other proteins such as ubiquitin ligases playing role in fine-tuning the cellular processes involved in survival and programmed cell death.

Proteasome dysfunction contributes to neurodegenerative diseases like Parkinson's and Alzheimer's. Disruption in proteasomal activity leads to aggregation of damaged proteins which can have pathological consequences. Cancer is another area where proteasomes play an important role; certain cancers show dysregulated proteasomal activity resulting in abnormal degradation of tumor suppressor proteins like p53. Understanding proteasome markers helps in diagnosing and evaluating these diseases linking proteasomes to both prognosis and potential therapeutic interventions.
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Product protocols

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Target data

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Publications (86)

Recent publications for all applications. Explore the full list and refine your search

Nature cardiovascular research 4:876-890 PubMed40562875

2025

AIMP3 maintains cardiac homeostasis by regulating the editing activity of methionyl-tRNA synthetase.

Applications

Unspecified application

Species

Unspecified reactive species

Anindhya S Das,Charles P Rabolli,Colton R Martens,Han-Kai Jiang,Yingshen Zhang,Aubree A Zimmer,Kevin Lin,Kedryn K Baskin,Juan D Alfonzo,Federica Accornero

The Journal of clinical investigation 135: PubMed39744939

2025

Cardiac fibroblast BAG3 regulates TGFBR2 signaling and fibrosis in dilated cardiomyopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Bryan Z Wang,Margaretha Aj Morsink,Seong Won Kim,Lori J Luo,Xiaokan Zhang,Rajesh Kumar Soni,Roberta I Lock,Jenny Rao,Youngbin Kim,Anran Zhang,Meraj Neyazi,Joshua M Gorham,Yuri Kim,Kemar Brown,Daniel M DeLaughter,Qi Zhang,Barbara McDonough,Josephine M Watkins,Katherine M Cunningham,Gavin Y Oudit,Barry M Fine,Christine E Seidman,Jonathan G Seidman,Gordana Vunjak-Novakovic

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 11:e2405907 PubMed39324579

2024

SNPs Give LACTB Oncogene-Like Functions and Prompt Tumor Progression via Dual-Regulating p53.

Applications

Unspecified application

Species

Unspecified reactive species

Guanyu Huang,Jiajun Zhang,Yu Xu,Fei Wu,Yiwei Fu,Xuelin Zhang,Hanxiao Yin,Yuanyuan You,Peng Zhao,Weihai Liu,Jingnan Shen,Junqiang Yin

Nature communications 15:2176 PubMed38467649

2024

YTHDF2 governs muscle size through a targeted modulation of proteostasis.

Applications

Unspecified application

Species

Unspecified reactive species

Christopher J Gilbert,Charles P Rabolli,Volha A Golubeva,Kristina M Sattler,Meifang Wang,Arsh Ketabforoush,W David Arnold,Christoph Lepper,Federica Accornero

Molecular cell 84:522-537.e8 PubMed38151017

2023

hRpn13 shapes the proteome and transcriptome through epigenetic factors HDAC8, PADI4, and transcription factor NF-κB p50.

Applications

Unspecified application

Species

Unspecified reactive species

Vasty Osei-Amponsa,Monika Chandravanshi,Xiuxiu Lu,Valentin Magidson,Sudipto Das,Thorkell Andresson,Marzena Dyba,Venkata R Sabbasani,Rolf E Swenson,Caroline Fromont,Biraj Shrestha,Yongmei Zhao,Michelle E Clapp,Raj Chari,Kylie J Walters

iScience 26:108292 PubMed38026225

2023

Guanylate-binding protein 1 modulates proteasomal machinery in ovarian cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Dhanir Tailor,Fernando Jose Garcia-Marques,Abel Bermudez,Sharon J Pitteri,Sanjay V Malhotra

Brain communications 5:fcad285 PubMed37953845

2023

α-Synuclein expression in response to bacterial ligands and metabolites in gut enteroendocrine cells: an proof of concept study.

Applications

Unspecified application

Species

Unspecified reactive species

Michael J Hurley,Elisa Menozzi,Sofia Koletsi,Rachel Bates,Matthew E Gegg,Kai-Yin Chau,Hervé M Blottière,Jane Macnaughtan,Anthony H V Schapira

Fluids and barriers of the CNS 20:70 PubMed37803468

2023

Proteasome inhibition protects blood-brain barrier P-glycoprotein and lowers Aβ brain levels in an Alzheimer's disease model.

Applications

Unspecified application

Species

Unspecified reactive species

Milica Vulin,Yu Zhong,Bryan J Maloney,Björn Bauer,Anika M S Hartz

GeroScience 46:1755-1773 PubMed37749371

2023

Induction of proteasomal activity in mammalian cells by lifespan-extending tRNA synthetase inhibitors.

Applications

Unspecified application

Species

Unspecified reactive species

Blaise L Mariner,Antonio S Rodriguez,Olivia C Heath,Mark A McCormick

Methods in molecular biology (Clifton, N.J.) 2712:135-148 PubMed37578702

2023

Analysis of Protein Degradation in Ferroptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Zhuojun Zhang,Lili Jiang
View all publications
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