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AB126287

Protein Carbonyl Content Assay Kit

5

(1 Review)

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(80 Publications)

Protein Carbonyl Content Assay Kit ab126287 is designed to provide a simple and accurate method of quantifying carbonyls in protein samples.
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Functional Studies - Protein Carbonyl Content Assay Kit (AB126287)
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Supplier Data

Functional Studies - Protein Carbonyl Content Assay Kit (AB126287)

Representative Data Obtained Using the Protein Carbonyl Content Assay Kit

Functional Studies - Protein Carbonyl Content Assay Kit (AB126287)
  • FuncS

Supplier Data

Functional Studies - Protein Carbonyl Content Assay Kit (AB126287)

Typical Standard Curve using BCA Protein Quantification Assay (ab102536)

Key facts

Detection method

Colorimetric

Sample types

Serum, Plasma, Cell culture extracts, Tissue Homogenate

Results type

Quantitative

Sensitivity

= 0.015 µM

Assay time

1h 30m

Assay Platform

Microplate reader

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Product details

Protein Carbonyl Content Assay Kit ab126287 is designed to provide a simple and accurate method of quantifying carbonyls in protein samples.

The protein carbonyl assay protocol is based on the reaction of DNPH with protein carbonyls. This is the most popular assay type for measuring protein carbonyl content. DNP hydrazones formed in this reaction are easily quantifiable at 375 nm absorbance.

When used with, for example a 1 mg (~15 nmol) sample, this protein carbonyl assay method has a detection limit of about 0.15 nmol carbonyl. For context, BSA typically contains approximately 1-3 nmol carbonyl/mg.

Protein carbonyl assay protocol summary:
- add DNPH to samples and incubate for 10 min
- add TCA to samples, incubate for 5 min, spin for 2 min, and discard supernatant
- wash pellet by sonicating in acetone, spin and remove acetone, repeat acetone wash step
- add Guanidine solution and resolubilize pellet, and transfer to plate
- analyze with a microplate reader to measure protein carbonyl content, also perform a protein assay (eg. BCA assay) on samples to establish protein concentration

The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.

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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
Please refer to protocols
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Protein carbonyls also known as carbonylated proteins serve as key markers for oxidative stress. They possess a carbonyl group introduced through oxidative modification of susceptible amino acid residues like lysine threonine proline and arginine. Protein carbonyls have no specific mass due to their varied formation but are widely detected across many cellular proteins. Carbonylation prominently expresses itself in intracellular and extracellular proteins affecting many tissues and organs. Laboratories often use the DNPH (dinitrophenylhydrazine) assay and other protein carbonylation assays to detect and quantify these modifications.
Biological function summary

Protein carbonylation alters protein function and structure. This process disrupts protein-protein interactions and impairs enzyme activity leading to protein dysfunction. Carbonylated proteins fail to perform effectively within cellular systems and accumulate in cells. These accumulations can form large aggregates which cells often recognize as damaged. Protein degradation systems struggle to efficiently degrade these aggregates which can lead to further cellular dysfunction.

Pathways

Protein carbonylation integrates into the oxidative stress response pathways. In particular it affects the proteostasis network by challenging the protein quality control systems which include chaperones and proteasomes. This can influence pathways like the ubiquitin-proteasome pathway as proteins like ubiquitin tag damaged proteins for degradation. Additionally carbonylated proteins intertwined with inflammatory pathways interact with proteins such as nuclear factor kappa B (NF-kB) contributing to pro-inflammatory signaling.

Protein carbonyls associate closely with neurodegenerative diseases like Alzheimer's disease and Parkinson's disease. The increase in oxidative stress in these conditions leads to the accumulation of carbonylated proteins which correlate with disease progression. In Alzheimer's disease oxidative damage results in the carbonylation of the amyloid precursor protein enhancing amyloid-beta accumulation. In Parkinson’s disease alpha-synuclein undergoes carbonylation promoting its aggregation into Lewy bodies. These protein associations highlight the significant role of protein carbonyls in disease pathology.
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Product protocols

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Target data

See full target information 2-[[[(2R)-2-ethylhexyl]carbamothioylamino]methyl]-4-nitrophenolate
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Publications (80)

Recent publications for all applications. Explore the full list and refine your search

GeroScience : PubMed40310487

2025

The effect of enhanced glycolysis on cardiac aging.

Applications

Unspecified application

Species

Unspecified reactive species

Anna Faakye,Kylene M Harold,Satoshi Matsuzaki,Atul Pranay,Maria F Mendez Garcia,Brooke L Loveland,Sandra N Rigsby,Frederick F Peelor,Craig Eyster,Benjamin F Miller,Timothy M Griffin,Michael Kinter,Ying Ann Chiao,Kenneth M Humphries

PNAS nexus 4:pgaf053 PubMed40007577

2025

Targeting sickle cell pathobiology and pain with novel transdermal curcumin.

Applications

Unspecified application

Species

Unspecified reactive species

Yugal Goel,Mya A Arellano,Raghda T Fouda,Natalie R Garcia,Reina A Lomeli,Daniel Kerr,Donovan A Argueta,Mihir Gupta,Graham J Velasco,Richard Prince,Probal Banerjee,Sirsendu Jana,Abdu I Alayash,Joel M Friedman,Kalpna Gupta

Frontiers in physiology 15:1427094 PubMed39224206

2024

Impact of cold storage on the oxygenation and oxidation reactions of red blood cells.

Applications

Unspecified application

Species

Unspecified reactive species

Tigist Kassa,Sirsendu Jana,Jin Hyen Baek,Abdu I Alayash

International journal of molecular sciences 25: PubMed39063101

2024

A New Tailored Nanodroplet Carrier of Astaxanthin Can Improve Its Pharmacokinetic Profile and Antioxidant and Anti-Inflammatory Efficacies.

Applications

Unspecified application

Species

Unspecified reactive species

Kumudesh Mishra,Nadin Khatib,Dinorah Barasch,Pradeep Kumar,Sharon Garti,Nissim Garti,Or Kakhlon

Cellular and molecular biology (Noisy-le-Grand, France) 70:85-91 PubMed38836676

2024

The combination of tetramethylpyrazine and vitamin A acid in the treatment of skin photoaging by activating the HIF-1α pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Weijun Chen,Jianyong Fan,Xuelian Zou,Yuansheng Wu

Cell 187:2359-2374.e18 PubMed38653240

2024

BCAA-nitrogen flux in brown fat controls metabolic health independent of thermogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Anthony R P Verkerke,Dandan Wang,Naofumi Yoshida,Zachary H Taxin,Xu Shi,Shuning Zheng,Yuka Li,Christopher Auger,Satoshi Oikawa,Jin-Seon Yook,Melia Granath-Panelo,Wentao He,Guo-Fang Zhang,Mami Matsushita,Masayuki Saito,Robert E Gerszten,Evanna L Mills,Alexander S Banks,Yasushi Ishihama,Phillip J White,Robert W McGarrah,Takeshi Yoneshiro,Shingo Kajimura

International journal of molecular sciences 25: PubMed38542166

2024

Beneficial Effects of Fibroblast Growth Factor-1 on Retinal Pigment Epithelial Cells Exposed to High Glucose-Induced Damage: Alleviation of Oxidative Stress, Endoplasmic Reticulum Stress, and Enhancement of Autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Hsin-Wei Huang,Chung-May Yang,Chang-Hao Yang

Heliyon 10:e28284 PubMed38533024

2024

Bezafibrate alleviates diabetes-induced spermatogenesis dysfunction by inhibiting inflammation and oxidative stress.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Mu,Ling-Bo Luo,Shu-Juan Wu,Yue Gao,Xiao-Lin Qin,Jing Zhao,Qian Liu,Jing Yang

International journal of molecular sciences 25: PubMed38474323

2024

Oxidative Stress-Mediated Repression of Virulence Gene Transcription and Biofilm Formation as Antibacterial Action of Essential Oil on .

Applications

Unspecified application

Species

Unspecified reactive species

Lingling Shi,Wei Lin,Yanling Cai,Feng Chen,Qian Zhang,Dongcheng Liang,Yu Xiu,Shanzhi Lin,Boxiang He

Journal of toxicology 2024:5539447 PubMed38410096

2024

Effect of Honey and Aqueous Garlic Extracts against Short-Term Exposure of Cigarette Tobacco Smoking in Mice: Histopathological and Biochemical Investigations.

Applications

Unspecified application

Species

Unspecified reactive species

Ziad Shraideh,Darwish Badran,Ahmed Alzbeede
View all publications
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Product promise

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