AB238536

Protein Carbonyl ELISA Kit

Name: Protein Carbonyl ELISA Kit (ab238536) | Abcam
Brand: Abcam Limited
SKU: AB238536
Price: 990 USD
Availability: InStock
Rating: 5 (1 reviews)

(1 Review)

(12 Publications)

Protein Carbonyl ELISA Kit ab238536 is designed for the quantitative measurement of protein carbonyls in plasma, serum, cell lysates or purified proteins.

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Indirect ELISA - Protein Carbonyl ELISA Kit (AB238536)

I-ELISA

Supplier Data

Indirect ELISA - Protein Carbonyl ELISA Kit (AB238536)

Amount of Protein Carbonyl Content for Cell Lysate and BSA Standard.

STO (MEF), HeLa and MDA-231 cells were sonicated in 25mM HEPES, pH 7.5, 150 mM NaCl, 10 mM MgCl2, 1 mM EDTA, 2% Glycerol. Cell Lysates and BSA Standard from Pierce BCA Protein Assay were diluted to 10 μg/mL with 1X PBS and coated onto a 96-well Protein Binding Plate. The protein carbonyl levels were determined as described in the Protein Carbonyl ELISA Protocol.

I-ELISA

Supplier Data

Indirect ELISA - Protein Carbonyl ELISA Kit (AB238536)

Typical Protein Carbonyl ELISA Standard Curve.

This standard curve is for demonstration only. A standard curve must be run with each protein carbonyl elisa experiment.

Key facts

Detection method

Colorimetric

Sample types

Cell Lysate, EDTA Plasma, Heparin Plasma, Serum, Tissue Lysate

Assay type

Cell-based

Results type

Quantitative

Assay Platform

Microplate reader

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Product details

Protein Carbonyl ELISA Kit (ab238536) is designed for the quantitative measurement of protein carbonyls in plasma, serum, cell lysates or purified proteins.

With this kit protein samples are first allowed to adsorb to wells of a 96-well plate and then react with dinitrophenylhydrazine (DNPH), which allows for derivatization of the carbonyl group. There are no concentration or precipitation steps, that contribute to sample loss. The kit allows detection of as little as 10 ug/mL using a standard microplate reader. The quantity of protein carbonyls in protein sample is determined by comparing its absorbance with that of a known reduced/oxidized BSA standard curve.

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What's included?

{ "values": { "96Test": { "sellingSize": "96 Test", "publicAssetCode":"ab238536-96Test", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Substrate Solution", "number":"AB238536-CMP11", "productcode":"" }, { "size":"1 x 500 µL", "name":"25X DNPH Solution", "number":"AB238536-CMP06", "productcode":"" }, { "size":"1 x 20 µL", "name":"Secondary Antibody, HRP Conjugate (1000X)", "number":"AB238536-CMP09", "productcode":"" }, { "size":"1 x 200 µL", "name":"Reduced BSA Standard", "number":"AB238536-CMP08", "productcode":"" }, { "size":"1 x 15 mL", "name":"2X DNPH Diluent", "number":"AB238536-CMP02", "productcode":"" }, { "size":"1 x 200 µL", "name":"Oxidized BSA Standard", "number":"AB238536-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Protein Binding Strip Well Plate", "number":"AB238536-CMP03", "productcode":"" }, { "size":"1 x 20 g", "name":"Blocking Reagent", "number":"AB238536-CMP05", "productcode":"" }, { "size":"1 x 20 µL", "name":"Anti-DNP Antibody (1000X)", "number":"AB238536-CMP04", "productcode":"" }, { "size":"1 x 100 mL", "name":"10X Wash Buffer", "number":"AB238536-CMP01", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB238536-CMP10", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

Multi

Appropriate long-term storage conditions

Multi

Storage information

Please refer to protocols

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Protein carbonyls also known as carbonylated proteins serve as key markers for oxidative stress. They possess a carbonyl group introduced through oxidative modification of susceptible amino acid residues like lysine threonine proline and arginine. Protein carbonyls have no specific mass due to their varied formation but are widely detected across many cellular proteins. Carbonylation prominently expresses itself in intracellular and extracellular proteins affecting many tissues and organs. Laboratories often use the DNPH (dinitrophenylhydrazine) assay and other protein carbonylation assays to detect and quantify these modifications.

Biological function summary

Protein carbonylation alters protein function and structure. This process disrupts protein-protein interactions and impairs enzyme activity leading to protein dysfunction. Carbonylated proteins fail to perform effectively within cellular systems and accumulate in cells. These accumulations can form large aggregates which cells often recognize as damaged. Protein degradation systems struggle to efficiently degrade these aggregates which can lead to further cellular dysfunction.

Pathways

Protein carbonylation integrates into the oxidative stress response pathways. In particular it affects the proteostasis network by challenging the protein quality control systems which include chaperones and proteasomes. This can influence pathways like the ubiquitin-proteasome pathway as proteins like ubiquitin tag damaged proteins for degradation. Additionally carbonylated proteins intertwined with inflammatory pathways interact with proteins such as nuclear factor kappa B (NF-kB) contributing to pro-inflammatory signaling.

Protein carbonyls associate closely with neurodegenerative diseases like Alzheimer's disease and Parkinson's disease. The increase in oxidative stress in these conditions leads to the accumulation of carbonylated proteins which correlate with disease progression. In Alzheimer's disease oxidative damage results in the carbonylation of the amyloid precursor protein enhancing amyloid-beta accumulation. In Parkinson's disease alpha-synuclein undergoes carbonylation promoting its aggregation into Lewy bodies. These protein associations highlight the significant role of protein carbonyls in disease pathology.

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Product protocols

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Target data

See full target information 2-[[[(2R)-2-ethylhexyl]carbamothioylamino]methyl]-4-nitrophenolate

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Publications (12)

Recent publications for all applications. Explore the full list and refine your search

Biology of sport 42:137-144 PubMed39758177

2025

Effects of vitamin D supplementation on oxidative stress and antioxidant enzyme after strenuous endurance exercise in healthy young men: a double-blind, placebo-controlled trial.

Applications

Unspecified application

Species

Unspecified reactive species

Cheng-Tse Yang,Pei-Wei Weng,Li-Hui Chien,Sunil Kumar,Ming-Ta Yang

Antioxidants (Basel, Switzerland) 13: PubMed39456511

2024

α-Terpineol Induces Shelterin Components TRF1 and TRF2 to Mitigate Senescence and Telomere Integrity Loss via A Telomerase-Independent Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Marianna Kapetanou,Sophia Athanasopoulou,Andreas Goutas,Dimitra Makatsori,Varvara Trachana,Efstathios Gonos

International journal of molecular sciences 25: PubMed38338809

2024

Effect of Selected Antioxidants on the In Vitro Aging of Human Fibroblasts.

Applications

Unspecified application

Species

Unspecified reactive species

Grzegorz Bartosz,Natalia Pieńkowska,Izabela Sadowska-Bartosz

GeroScience 45:3549-3560 PubMed37498479

2023

Basal autophagic flux measured in blood correlates positively with age in adults at increased risk of type 2 diabetes.

Applications

Unspecified application

Species

Unspecified reactive species

Julien Bensalem,Xiao Tong Teong,Kathryn J Hattersley,Leanne K Hein,Célia Fourrier,Kai Liu,Amy T Hutchison,Leonie K Heilbronn,Timothy J Sargeant

International journal of molecular sciences 24: PubMed37446125

2023

Weak Hypotensive Effect of Chronic Administration of the Dual FAAH/MAGL Inhibitor JZL195 in Spontaneously Hypertensive Rats as Revealed by Area under the Curve Analysis.

Applications

Unspecified application

Species

Unspecified reactive species

Marek Toczek,Piotr Ryszkiewicz,Patryk Remiszewski,Eberhard Schlicker,Anna Krzyżewska,Hanna Kozłowska,Barbara Malinowska

Scientific reports 13:8304 PubMed37221295

2023

Sting and p53 DNA repair pathways are compromised in Alzheimer's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Thomas J Nelson,Yunhui Xu

Toxics 11: PubMed36668787

2023

Can Maternal Exposure to Air Pollution Affect Post-Natal Liver Development?

Applications

Unspecified application

Species

Unspecified reactive species

Yong Song,Ling Chen,Ellen Bennett,Amanda J Wheeler,Katherine Southam,Seiha Yen,Fay Johnston,Graeme R Zosky

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 9:e2203242 PubMed36002317

2022

Hemithioindigo-Based Visible Light-Activated Molecular Machines Kill Bacteria by Oxidative Damage.

Applications

Unspecified application

Species

Unspecified reactive species

Ana L Santos,Alexis van Venrooy,Anna K Reed,Aaron M Wyderka,Víctor García-López,Lawrence B Alemany,Antonio Oliver,George P Tegos,James M Tour

Antioxidants (Basel, Switzerland) 11: PubMed35326118

2022

Antioxidant and Antiaging Properties of a Novel Synergistic Nutraceutical Complex: Readouts from an In Cellulo Study and an In Vivo Prospective, Randomized Trial.

Applications

Unspecified application

Species

Unspecified reactive species

Sophia Athanasopoulou,Marianna Kapetanou,Michel Georges Magouritsas,Nikoletta Mougkolia,Polykseni Taouxidou,Michael Papacharalambous,Fotios Sakellaridis,Efstathios Gonos

Biology 11: PubMed35205143

2022

Sustained Functioning Impairments and Oxidative Stress with Neurobehavioral Dysfunction Associated with Oral Nicotine Exposure in the Brain of a Murine Model of Ehrlich Ascites Carcinoma: Modifying the Antioxidant Role of .

Applications

Unspecified application

Species

Unspecified reactive species

Amany Abdel-Rahman Mohamed,Khlood M El Bohy,Gihan G Moustafa,Hesham H Mohammed,Mohamed M M Metwally,Heba El Desoukey Mohammed,Mohamed A Nassan,Taghred M Saber

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Protein Carbonyl ELISA Kit

Indirect ELISA - Protein Carbonyl ELISA Kit (AB238536)

Indirect ELISA - Protein Carbonyl ELISA Kit (AB238536)

Key facts

Detection method

Sample types

Assay type

Results type

Assay Platform

Complementary Products

Assay Kits

Secondary Antibodies

Assay Kits

Primary Antibodies

Product details

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (12)

Product promise