Protein Synthesis Assay Kit (Green)

• Flow Cyt

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Flow Cytometry - Protein Synthesis Assay Kit (Green) (AB239725)

Inhibitory effect of Cycloheximide on nascent polypeptides synthesis.

HeLa (10⁵ cells/ ml) and Jurkat (1X10⁶ cells/ml) cells respectively were pre-treated with vehicle or Cycloheximide for 30 min at 37°C. Subsequently, cells were incubated for additional 30 min with fresh aliquots of media containing either Protein Label or Protein Label and Cycloheximide. Cells were then processed and analyzed by Microscopy and FACS according to the kit protocol. (B) FACS analysis of negative control (white), background (Azide only, blue), positive control (Protein Label, green) and Cycloheximide-treated (pink) cell populations. Signal measured in FL-1 channel clearly shows the inhibitory effect of Cycloheximide on nascent polypeptides synthesis.

• Fluorescence Microscopy

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Fluorescence Microscopy - Protein Synthesis Assay Kit (Green) (AB239725)

Inhibitory effect of Cycloheximide on nascent polypeptides synthesis.

HeLa (10⁵ cells/ ml) and Jurkat (1X10⁶ cells/ml) cells respectively were pre-treated with vehicle or Cycloheximide for 30 min at 37°C. Subsequently, cells were incubated for additional 30 min with fresh aliquots of media containing either Protein Label or Protein Label and Cycloheximide. Cells were then processed and analyzed by Microscopy and FACS according to the kit protocol. (A) Green fluorescence (upper panel) corresponds to de novo synthesized polypeptides whereas bottom panel shows the inhibitory effect of Cycloheximide on protein biosynthesis. Nuclear staining in both panels confirms that green signal is a result of Protein Label incorporation.

• Other

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Other - Protein Synthesis Assay Kit (Green) (AB239725)

Representative image of Protein Synthesis Assay Kit (Green) ab239725

Components shown from left to right :

- 400X Protein Label

- 1000X DAPI

- 100X Cycloheximide

- Fluorescent Azide II

- Reducing Agent

- 100X Copper Reagent

- Fixative Solution

- 10X Permeabilization Buffer

- Wash Buffer IV

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit.