Protein Synthesis Assay Kit (ab239725) uses a novel and robust chemical method based on an alkyne containing and cell permeable analog of puromycin, O-Propargyl-puromycin (OP-puro).
Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
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- Frontiers in pharmacology 13:10274412022Matrine, a potential c-Myc inhibitor, suppresses ribosome biogenesis and nucleotide metabolism in myeloid leukemia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWang-Jing Zhong,Lingdi Ma,Fanfan Yang,Jialin Cao,Junyu Tan,Bohong LiPubMed 36339620
- NPJ Parkinson's disease 8:152022High content screening and proteomic analysis identify a kinase inhibitor that rescues pathological phenotypes in a patient-derived model of Parkinson's disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNasia Antoniou,Kanella Prodromidou,Georgia Kouroupi,Ioanna Boumpoureka,Martina Samiotaki,George Panayotou,Maria Xilouri,Ismini Kloukina,Leonidas Stefanis,Regis Grailhe,Era Taoufik,Rebecca MatsasPubMed 35149677
- EMBO reports 22:e530482021Ronin governs the metabolic capacity of the embryonic lineage for post-implantation development.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKirill Salewskij,Theresa Gross-Thebing,Elizabeth Ing-Simmons,Binyamin Duethorn,Bettina Rieger,Rui Fan,Rui Chen,Niraimathi Govindasamy,Heike Brinkmann,Ludmila Kremer,Nannette Kuempel-Rink,Karina Mildner,Dagmar Zeuschner,Martin Stehling,Marion Dejosez,Thomas P Zwaka,Hans R Schöler,Karin B Busch,Juan M Vaquerizas,Ivan BedzhovPubMed 34515391
- Cytotherapy 22:106-1132020Rhynchophylline promotes stem cell autonomous metabolic homeostasis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYuji Kaneko et. al.PubMed 31983606
Key facts
- Detection method
Fluorescent
- Sample types
Suspension cells, Adherent cells
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Protein Synthesis Assay Kit (ab239725) uses a novel and robust chemical method based on an alkyne containing and cell permeable analog of puromycin, O-Propargyl-puromycin (OP-puro).
Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
Key facts
- Detection method
Fluorescent
- Sample types
Suspension cells, Adherent cells
- Assay Platform
Flow cytometer, Fluorescence microscope
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
-20°C
- Appropriate long-term storage conditions
-20°C
- Storage information
-20°C
Notes
Protein Synthesis Assay Kit (ab239725) utilizes a novel and robust chemical method based on an alkyne containing and cell permeable analog of puromycin, O-Propargyl-puromycin (OP-puro). Once inside the cell, OP-puro stops translation by forming covalent conjugates with nascent polypeptide chains.
Truncated polypeptides are rapidly turned over by the proteasome and can be detected based on a click reaction with the fluorescent azide. Unlike methionine analogs, OP-puro does not require methionine-free conditions and can be used to label nascent proteins directly in the cell culture.
This product is manufactured by BioVision, an Abcam company and was previously called K459 EZClick™ Global Protein Synthesis Assay Kit (FACS/Microscopy), Green Fluorescence. K459-100 is the same size as the 100 test size of ab239725.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
Fluorescence Microscopy - Protein Synthesis Assay Kit (Green) (ab239725)
Inhibitory effect of Cycloheximide on nascent polypeptides synthesis.
HeLa (105 cells/ ml) and Jurkat (1X106 cells/ml) cells respectively were pre-treated with vehicle or Cycloheximide for 30 min at 37°C. Subsequently, cells were incubated for additional 30 min with fresh aliquots of media containing either Protein Label or Protein Label and Cycloheximide. Cells were then processed and analyzed by Microscopy and FACS according to the kit protocol. (A) Green fluorescence (upper panel) corresponds to de novo synthesized polypeptides whereas bottom panel shows the inhibitory effect of Cycloheximide on protein biosynthesis. Nuclear staining in both panels confirms that green signal is a result of Protein Label incorporation.
Flow Cytometry - Protein Synthesis Assay Kit (Green) (ab239725)
HeLa (105 cells/ ml) and Jurkat (1X106 cells/ml) cells respectively were pre-treated with vehicle or Cycloheximide for 30 min at 37°C. Subsequently, cells were incubated for additional 30 min with fresh aliquots of media containing either Protein Label or Protein Label and Cycloheximide. Cells were then processed and analyzed by Microscopy and FACS according to the kit protocol. (B) FACS analysis of negative control (white), background (Azide only, blue), positive control (Protein Label, green) and Cycloheximide-treated (pink) cell populations. Signal measured in FL-1 channel clearly shows the inhibitory effect of Cycloheximide on nascent polypeptides synthesis.
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