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AB241032

Protein Tyrosine Phosphatase Assay Kit

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(1 Publication)

Protein Tyrosine Phosphatase Assay Kit (ab241032) enables rapid measurement of protein tyrosine phosphatase (PTPase) activity, utilizing a fluorogenic protein phosphatase substrate that is converted into a highly fluorescent product (Ex/Em = 368/460 nm).

View Alternative Names

Low molecular weight phosphotyrosine protein phosphatase, LMW-PTP, LMW-PTPase, Adipocyte acid phosphatase, Low molecular weight cytosolic acid phosphatase, Red cell acid phosphatase 1, ACP1

3 Images
Functional Studies - Protein Tyrosine Phosphatase Assay Kit (AB241032)
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Functional Studies - Protein Tyrosine Phosphatase Assay Kit (AB241032)

Reaction kinetics of PTPase Substrate metabolism in homogenates of human placenta (5 μg) and rat prefrontal cortex (10 μg) in the presence and absence of the PTPase inhibitor suramin.

Functional Studies - Protein Tyrosine Phosphatase Assay Kit (AB241032)
  • FuncS

Unknown

Functional Studies - Protein Tyrosine Phosphatase Assay Kit (AB241032)

Quantification of PTPase activity in samples (mean ± SEM of 4 or more independent replicates). Assays were performed according to the kit protocol.

Functional Studies - Protein Tyrosine Phosphatase Assay Kit (AB241032)
  • FuncS

Unknown

Functional Studies - Protein Tyrosine Phosphatase Assay Kit (AB241032)

Fluorescence Standard curve. One mole of Fluorescence Standard corresponds to the metabolism of one mole of PTPase Substrate with release of one mole free inorganic phosphate.

Key facts

Detection method

Fluorescent

Sample types

Tissue Homogenate, Cell Lysate, Purified protein

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Enzyme activity assay": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Protein Tyrosine Phosphatase Assay Kit (ab241032) enables rapid measurement of protein tyrosine phosphatase (PTPase) activity, utilizing a fluorogenic protein phosphatase substrate that is converted into a highly fluorescent product (Ex/Em = 368/460 nm). A broad-spectrum PTPase inhibitor is provided for verification of specific activity in complex biological matrices, where serine/threonine phosphatases may contribute to substrate metabolism. Unlike ELISAs or malachite green-based approaches, the assay is homogeneous, continuous and does not require complicated sample processing or desalting to eliminate free phosphate. The assay is simple to perform, high-throughput adaptable and can detect a minimum of 0.1 mU PTPase activity.

This product is manufactured by BioVision, an Abcam company and was previously called K829 Protein Tyrosine Phosphatase Activity Assay Kit (Fluorometric). K829-100 is the same size as the 100 test size of ab241032.

The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.

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What's included?

{ "values": { "100Test": { "sellingSize": "100 Test", "publicAssetCode":"ab241032-100Test", "assetComponentDetails": [ { "size":"1 x 1 Vial", "name":"PTPase Positive Control", "number":"AB241032-CMP05", "productcode":"" }, { "size":"1 x 300 µL", "name":"Reducing Agent II", "number":"AB241032-CMP02", "productcode":"" }, { "size":"1 x 25 mL", "name":"PTPase Assay Buffer", "number":"AB241032-CMP03", "productcode":"" }, { "size":"1 x 1 Each", "name":"Inhibitor (Suramin)", "number":"AB241032-CMP04", "productcode":"" }, { "size":"1 x 1 Each", "name":"PTPase Substrate", "number":"AB241032-CMP06", "productcode":"" }, { "size":"1 x 50 µL", "name":"PTPase Fluorescence Standard", "number":"AB241032-CMP01", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Acid phosphatase also known as ACP1 or protein tyrosine phosphatase is an enzyme with a mass of approximately 18 kDa. It works by removing phosphate groups from tyrosine residues on proteins which modulates protein function. This phosphatase is expressed in various tissues including the pancreas and liver and is present in immune cells. In scientific studies ACP1 can also be studied in HEK 293 cells a commonly used human cell line for in vitro assays.
Biological function summary

The acid phosphatase enzyme plays an important role in cellular signaling and regulation of metabolic processes. It belongs to the enzyme family of protein tyrosine phosphatases which are key in dephosphorylating proteins and regulating signal transduction pathways. ACP1 does not function as part of a large protein complex but it still affects cellular processes by interacting with multiple molecular partners.

Pathways

The acid phosphatase enzyme is involved in key cellular signaling pathways including the insulin signaling pathway and T-cell receptor signaling. These pathways influence glucose metabolism and immune response. In these pathways ACP1 interacts with proteins such as the insulin receptor substrate and Lck influencing phosphorylation states and cellular activities.

Acid phosphatase has connections to conditions like autoimmune diseases and type 2 diabetes. In autoimmune disorders altered ACP1 activity can affect immune cell signaling. Studies link increased enzyme activity or genetic variations to these conditions impacting associated proteins like the insulin receptor in diabetes or TCR complex proteins in immune dysfunctions providing insight into how aberrations in ACP1 can contribute to disease pathogenesis.
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Product protocols

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Target data

Acts on tyrosine phosphorylated proteins, low-MW aryl phosphates and natural and synthetic acyl phosphates with differences in substrate specificity between isoform 1 and isoform 2.. Isoform 3. Does not possess phosphatase activity.
See full target information ACP1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

ACS omega 10:15511-15524 PubMed40290999

2025

Activation of the Insulin Receptor by Extract and Identification of Sarcocyanidin A as a Novel Active Compound.

Applications

Unspecified application

Species

Unspecified reactive species

Ayala Wollman,Rania Hasib Afana,Shmuel Carmeli,Tovit Rosenzweig
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Product promise

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