Protein Tyrosine Phosphatase Assay Kit (ab241032) enables rapid measurement of protein tyrosine phosphatase (PTPase) activity, utilizing a fluorogenic protein phosphatase substrate that is converted into a highly fluorescent product (Ex/Em = 368/460 nm).
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Key facts
- Detection method
Fluorescent
Target data
Function
Acts on tyrosine phosphorylated proteins, low-MW aryl phosphates and natural and synthetic acyl phosphates. Isoform 3 does not possess phosphatase activity.
Alternative names
Low molecular weight phosphotyrosine protein phosphatase, LMW-PTP, LMW-PTPase, Adipocyte acid phosphatase, Low molecular weight cytosolic acid phosphatase, Red cell acid phosphatase 1, ACP1
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Protein Tyrosine Phosphatase Assay Kit (ab241032) enables rapid measurement of protein tyrosine phosphatase (PTPase) activity, utilizing a fluorogenic protein phosphatase substrate that is converted into a highly fluorescent product (Ex/Em = 368/460 nm).
Alternative names
Low molecular weight phosphotyrosine protein phosphatase, LMW-PTP, LMW-PTPase, Adipocyte acid phosphatase, Low molecular weight cytosolic acid phosphatase, Red cell acid phosphatase 1, ACP1
Key facts
- Detection method
Fluorescent
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
-20°C
- Appropriate long-term storage conditions
-20°C
- Storage information
-20°C
Notes
Protein Tyrosine Phosphatase Assay Kit (ab241032) enables rapid measurement of protein tyrosine phosphatase (PTPase) activity, utilizing a fluorogenic protein phosphatase substrate that is converted into a highly fluorescent product (Ex/Em = 368/460 nm). A broad-spectrum PTPase inhibitor is provided for verification of specific activity in complex biological matrices, where serine/threonine phosphatases may contribute to substrate metabolism. Unlike ELISAs or malachite green-based approaches, the assay is homogeneous, continuous and does not require complicated sample processing or desalting to eliminate free phosphate. The assay is simple to perform, high-throughput adaptable and can detect a minimum of 0.1 mU PTPase activity.
This product is manufactured by BioVision, an Abcam company and was previously called K829 Protein Tyrosine Phosphatase Activity Assay Kit (Fluorometric). K829-100 is the same size as the 100 test size of ab241032.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Acid phosphatase also known as ACP1 or protein tyrosine phosphatase is an enzyme with a mass of approximately 18 kDa. It works by removing phosphate groups from tyrosine residues on proteins which modulates protein function. This phosphatase is expressed in various tissues including the pancreas and liver and is present in immune cells. In scientific studies ACP1 can also be studied in HEK 293 cells a commonly used human cell line for in vitro assays.
- Biological function summary
The acid phosphatase enzyme plays an important role in cellular signaling and regulation of metabolic processes. It belongs to the enzyme family of protein tyrosine phosphatases which are key in dephosphorylating proteins and regulating signal transduction pathways. ACP1 does not function as part of a large protein complex but it still affects cellular processes by interacting with multiple molecular partners.
- Pathways
The acid phosphatase enzyme is involved in key cellular signaling pathways including the insulin signaling pathway and T-cell receptor signaling. These pathways influence glucose metabolism and immune response. In these pathways ACP1 interacts with proteins such as the insulin receptor substrate and Lck influencing phosphorylation states and cellular activities.
- Associated diseases and disorders
Acid phosphatase has connections to conditions like autoimmune diseases and type 2 diabetes. In autoimmune disorders altered ACP1 activity can affect immune cell signaling. Studies link increased enzyme activity or genetic variations to these conditions impacting associated proteins like the insulin receptor in diabetes or TCR complex proteins in immune dysfunctions providing insight into how aberrations in ACP1 can contribute to disease pathogenesis.
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3 product images
Functional Studies - Protein Tyrosine Phosphatase Assay Kit (ab241032)
Fluorescence Standard curve. One mole of Fluorescence Standard corresponds to the metabolism of one mole of PTPase Substrate with release of one mole free inorganic phosphate.
Functional Studies - Protein Tyrosine Phosphatase Assay Kit (ab241032)
Reaction kinetics of PTPase Substrate metabolism in homogenates of human placenta (5 μg) and rat prefrontal cortex (10 μg) in the presence and absence of the PTPase inhibitor suramin.
Functional Studies - Protein Tyrosine Phosphatase Assay Kit (ab241032)
Quantification of PTPase activity in samples (mean ± SEM of 4 or more independent replicates). Assays were performed according to the kit protocol.
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