Pyridoxal 5'-phosphate (Vit B6) Assay Kit (Fluorometric) (ab273312) provides a quick, specific and easy method for measuring Pyridoxal 5'-phosphate (PLP) concentrations in a wide variety of samples.
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- International journal of molecular sciences 23:2022Metabolic Rewiring by Human Placenta-Derived Mesenchymal Stem Cell Therapy Promotes Rejuvenation in Aged Female Rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKyeoung-Hwa Kim,Kyung-Ah LeePubMed 35008991
Key facts
- Detection method
Fluorescent
- Sample types
Tissue, Other biological fluids
- Assay type
Quantitative
Alternative names
Pyridoxal, Pyridoxamine, Pyridoxine
What's included?
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Pyridoxal 5'-phosphate (Vit B6) Assay Kit (Fluorometric) (ab273312) provides a quick, specific and easy method for measuring Pyridoxal 5'-phosphate (PLP) concentrations in a wide variety of samples.
Alternative names
Pyridoxal, Pyridoxamine, Pyridoxine
Key facts
- Detection method
Fluorescent
- Sample types
Tissue, Other biological fluids
- Assay type
Quantitative
- Assay Platform
Microplate
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
-20°C
- Appropriate long-term storage conditions
-20°C
- Storage information
-20°C
Notes
Pyridoxal 5'-phosphate (Vit B6) Assay Kit (Fluorometric) (ab273312) provides a quick, specific and easy method for measuring Pyridoxal 5'-phosphate (PLP) concentrations in a wide variety of samples.
In this assay, PLP reacts with a PLP-dependent enzyme thereby converting the substrate into an intermediate, which will further react with a probe to produce a strong fluorometric signal (Ex/Em = 535/587 nm). The kit is simple, easy to use, sensitive and high-throughput adaptable. It can detect as low as 0.2 pmol/well of PLP in biological samples.
This product is manufactured by BioVision, an Abcam company and was previously called K2009 Pyridoxal 5'-phosphate (Vit B6) Fluorometric Assay Kit. K2009-100 is the same size as the 100 test size of ab273312.
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Functional Studies - Pyridoxal 5'-phosphate (Vit B6) Assay Kit (Fluorometric) (ab273312)
PLP standard curve.
Typical standard curve – data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.
Functional Studies - Pyridoxal 5'-phosphate (Vit B6) Assay Kit (Fluorometric) (ab273312)
Example data.
Corrected kinetic curve for Blank, orange juice, human serum and rat liver lysate.
Functional Studies - Pyridoxal 5'-phosphate (Vit B6) Assay Kit (Fluorometric) (ab273312)
Example data.
Estimation of PLP in orange juice (5 μL of 100X dilution), human serum (4 μL) and rat liver lysate (5 μL of 10X dilution). PLP concentrations were 4.36± 0.40 μM in orange juice, 15.98 ± 0.58 nM in human serum and 79.0 ± 4.0 pmol/mg in rat liver lysate samples. Assays were performed following the kit protocol.
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