Pyrophosphate Assay Kit (Colorimetric / Fluorometric)

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Functional Studies - Pyrophosphate Assay Kit (Colorimetric / Fluorometric) (AB234040)

Example Colorimetric Standard Curve

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Functional Studies - Pyrophosphate Assay Kit (Colorimetric / Fluorometric) (AB234040)

Quantification of pyrophosphate in de-proteinized undiluted human serum and pooled plasma.

Quantification of pyrophosphate in 20 μL of de-proteinized undiluted human serum and pooled plasma.

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Functional Studies - Pyrophosphate Assay Kit (Colorimetric / Fluorometric) (AB234040)

Spike and recovery in normal human plasma.

Spike and recovery in 20 μL of normal human plasma. Plasma samples were spiked with 0.06 nmol of PPi Standard and assayed according to kit protocol yielding 96% PPi recovery.

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Functional Studies - Pyrophosphate Assay Kit (Colorimetric / Fluorometric) (AB234040)

Pyrophosphate measured in HepG2 and HeLa cell lysates.

Cells were extracted directly in the Assay Buffer.

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Functional Studies - Pyrophosphate Assay Kit (Colorimetric / Fluorometric) (AB234040)

Example Fluorometric Standard Curve

• Schematic Diagram

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Schematic Diagram - Pyrophosphate Assay Kit (Colorimetric / Fluorometric) (AB234040)

Representative image of Pyrophosphate Assay Kit (Colorimetric / Fluorometric) ab234040

Components shown from left to right :

- OxiRed Probe

- Pyrophosphate Standard

- Enzyme Mix X

- PEP (2 vials)

- Developer Mix A

- Assay Buffer 15

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit.