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AB65342

Pyruvate Assay Kit

5

(2 Reviews)

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(105 Publications)

Pyruvate Assay Kit (Colorimetric/Fluorometric) ab65342 provides a simple, direct and automation-ready procedure for measuring pyruvate concentration in various biological samples such as blood, cells, culture and fermentation media. Readout on any colorimetric (570 nm) or fluorometric (Ex/Em = 535/587 nm) plate reader.

- Complete kit including standard curve for quantitation
- Cited in >75 publications
- Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
7 Images
Functional Studies - Pyruvate Assay Kit (AB65342)
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Lab

Functional Studies - Pyruvate Assay Kit (AB65342)

Pyruvate measured fluorometrically in biological fluids showing quantity (nmol) per mL of tested sample

Functional Studies - Pyruvate Assay Kit (AB65342)
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Supplier Data

Functional Studies - Pyruvate Assay Kit (AB65342)

Pyruvate fluorometric standard curve.

Functional Studies - Pyruvate Assay Kit (AB65342)
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Lab

Functional Studies - Pyruvate Assay Kit (AB65342)

Pyruvate measured fluorometrically in biological fluids showing quantity (nmol) per mln of tested cells

Functional Studies - Pyruvate Assay Kit (AB65342)
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Functional Studies - Pyruvate Assay Kit (AB65342)

Pyruvate levels under manganese stress and iron supplementation were measured with ab65342 and the results were plotted as a function of normalized absorbance at 450 nm per milligram of cellular proteins.

Kaur, Gursharan et al., Scientific reports vol. 7,1 11645., Fig 2, doi:10.1038/s41598-017-12004-3

Functional Studies - Pyruvate Assay Kit (AB65342)
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Supplier Data

Functional Studies - Pyruvate Assay Kit (AB65342)

Pyruvate colorimetric standard curve.

Functional Studies - Pyruvate Assay Kit (AB65342)
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Lab

Functional Studies - Pyruvate Assay Kit (AB65342)

Pyruvate measured colourimetrically in biological fluids showing quantity (nmol) per mL of tested sample

Schematic Diagram - Pyruvate Assay Kit (AB65342)
  • Schematic Diagram

Supplier Data

Schematic Diagram - Pyruvate Assay Kit (AB65342)

Representative image of Pyruvate Assay Kit ab65342

Components shown from left to right :

- OxiRed Probe

- Pyruvate Standard

- Developer Mix A

- Assay Buffer 4

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit.

Key facts

Detection method

Colorimetric/Fluorometric

Sample types

Urine, Plasma, Cell culture extracts, Tissue Extracts, Serum, Other biological fluids

Assay type

Quantitative

Sensitivity

> 1 µM

Range

1 - 200 µM

Assay time

40m

Assay Platform

Microplate reader

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Product details

Pyruvate Assay Kit (Colorimetric/Fluorometric) ab65342 provides a simple, direct and automation-ready procedure for measuring pyruvate concentration in various biological samples such as blood, cells, culture and fermentation media, etc.

In the pyruvate assay protocol, pyruvate is oxidized by pyruvate oxidase via enzyme reactions to generate color (λ= 570 nm) and fluorescence (at Ex/Em = 535/587 nm). Since the color or fluorescence intensity is proportional to pyruvate content, the pyruvate concentration can be accurately measured.

The kit detects 1-200 μM pyruvate.

Pyruvate assay protocol summary:
- add samples and standards to wells
- add reaction mix and incubate for 30 min at room temp
- analyze with microplate reader

This product is manufactured by BioVision, an Abcam company and was previously called K609 Pyruvate Colorimetric/Fluorometric Assay Kit. K609-100 is the same size as the 100 test size of ab65342.

The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.

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What's included?

{ "values": { "100Test": { "sellingSize": "100 Test", "publicAssetCode":"ab65342-100Test", "assetComponentDetails": [ { "size":"1 x 100 µL", "name":"Pyruvate Standard", "number":"AB65342-CMP04", "productcode":"" }, { "size":"1 x 0.2 mL", "name":"OxiRed™ Probe", "number":"AB65342-CMP03", "productcode":"AB309437" }, { "size":"1 x 1 Vial", "name":"Developer Mix A", "number":"AB65342-CMP02", "productcode":"AB309440" }, { "size":"1 x 25 mL", "name":"Assay Buffer 4", "number":"AB65342-CMP01", "productcode":"AB323949" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Pyruvate often called pyruvic acid or 'pyr' is an important intermediate metabolite in cellular respiration. It has a molecular mass of 88.06 g/mol and plays an integral role in the metabolic process. Pyruvate is present in virtually all living cells including plants and animals. The compound forms during glycolysis through the breakdown of glucose and can undergo several conversions serving as a precursor for various biochemical pathways. Potassium pyruvate is the salt form commonly used to study pyruvate's functions in the lab.
Biological function summary

This compound serves essential roles in energy production and biosynthesis. As part of the pyruvate dehydrogenase complex pyruvate connects glycolysis to the Krebs cycle by enabling the conversion to acetyl-CoA. This conversion is necessary for the aerobic respiration process. Furthermore pyruvate contributes to gluconeogenesis where it influences the creation of glucose from non-carbohydrate sources important during periods of fasting or intense exercise.

Pathways

Pyruvate interacts significantly in metabolic pathways like glycolysis and the citric acid cycle emphasizing cellular energy metabolism. In glycolysis pyruvate acts as the endpoint molecule while in the citric acid cycle it enters as acetyl-CoA maintaining the cycle in motion. The regulation of these pathways involves various enzymes and proteins like pyruvate kinase and lactate dehydrogenase associate with pyruvate’s metabolic roles dictating transitions between aerobic and anaerobic states.

Alterations in pyruvate metabolism link closely to cancer and diabetes. Cancer cells often exhibit increased glycolytic rates known as the Warburg effect where pyruvate's conversion influences tumor growth. Pyruvate kinase M2 a protein variant is notably expressed in cancer cells and affects pyruvate's fate. In diabetes impaired pyruvate metabolism impacts insulin regulation contributing to the disease's metabolic complications. High levels of pyruvate can lead to lactic acidosis emphasizing the balance required in normal metabolic functioning.
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Product protocols

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Target data

See full target information Pyruvate
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Publications (105)

Recent publications for all applications. Explore the full list and refine your search

Biochemistry and biophysics reports 42:102048 PubMed40486495

2025

β-suppressor protein 1 (ARRB1)-△exon13 modulates the progression of glioblastoma via combination with glycolysis-related proteins.

Applications

Unspecified application

Species

Unspecified reactive species

Zi-Long Wei,Shuo Han,Dong-Hua Han,Xue-Tao Li,Yu-Lun Huang,Zhi-Min Wang

Cell death & disease 16:316 PubMed40253387

2025

Endoplasmic reticulum stress related super-enhancers suppress cuproptosis via glycolysis reprogramming in lung adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Gu,Hongchang Wang,Wentao Xue,Linjia Zhu,Chenghao Fu,Wenhao Zhang,Guang Mu,Yang Xia,Ke Wei,Jun Wang

Asian Pacific journal of cancer prevention : APJCP 26:757-766 PubMed40156391

2025

The Synergistic and Anticancer Potential of Withania Somnifera (Ashwagandha) Ethanol Extract as an Adjuvant with Doxorubicin in MCF7 Breast Cancer Cell Line.

Applications

Unspecified application

Species

Unspecified reactive species

Emad M Elzayat,Ghada E Elsamahy,Ghada H Mansour,Ahmed A El-Sherif,Nourhan Hassan

JCI insight 9: PubMed39315548

2024

Pyruvate metabolism dictates fibroblast sensitivity to GLS1 inhibition during fibrogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Greg Contento,Jo-Anne Am Wilson,Brintha Selvarajah,Manuela Platé,Delphine Guillotin,Valle Morales,Marcello Trevisani,Vanessa Pitozzi,Katiuscia Bianchi,Rachel C Chambers

Journal of biochemical and molecular toxicology 38:e23854 PubMed39287333

2024

Malate dehydrogenase-2 inhibition shields renal tubular epithelial cells from anoxia-reoxygenation injury by reducing reactive oxygen species.

Applications

Unspecified application

Species

Unspecified reactive species

Georgios Pissas,Maria Tziastoudi,Maria Divani,Christina Poulianiti,Maria Anna Polyzou Konsta,Evangelos Lykotsetas,Vasilios Liakopoulos,Ioannis Stefanidis,Theodoros Eleftheriadis

eLife 13: PubMed39269443

2024

The bacterial quorum sensing signal 2'-aminoacetophenone rewires immune cell bioenergetics through the Ppargc1a/Esrra axis to mediate tolerance to infection.

Applications

Unspecified application

Species

Unspecified reactive species

Arijit Chakraborty,Arunava Bandyopadhaya,Vijay K Singh,Filip Kovacic,Sujin Cha,William M Oldham,A Aria Tzika,Laurence G Rahme

Nature communications 15:6755 PubMed39117659

2024

Histone-methyltransferase KMT2D deficiency impairs the Fanconi anemia/BRCA pathway upon glycolytic inhibition in squamous cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Liu,Hongchao Cao,Jing Wang,Areeg Elmusrati,Bing Han,Wei Chen,Ping Zhou,Xiyao Li,Stephen Keysar,Antonio Jimeno,Cun-Yu Wang

Nature communications 15:4216 PubMed38760394

2024

Mycobacterium tuberculosis suppresses host antimicrobial peptides by dehydrogenating L-alanine.

Applications

Unspecified application

Species

Unspecified reactive species

Cheng Peng,Yuanna Cheng,Mingtong Ma,Qiu Chen,Yongjia Duan,Shanshan Liu,Hongyu Cheng,Hua Yang,Jingping Huang,Wenyi Bu,Chenyue Shi,Xiangyang Wu,Jianxia Chen,Ruijuan Zheng,Zhonghua Liu,Zhe Ji,Jie Wang,Xiaochen Huang,Peng Wang,Wei Sha,Baoxue Ge,Lin Wang

Cell communication and signaling : CCS 22:93 PubMed38302971

2024

Mechanosensitive channel of large conductance enhances the mechanical stretching-induced upregulation of glycolysis and oxidative metabolism in Schwann cells.

Applications

Unspecified application

Species

Unspecified reactive species

Fangzhen Shan,Nannan Zhang,Xiaoying Yao,Yi Li,Zihao Wang,Chuanji Zhang,Yuzhong Wang

Heliyon 10:e24421 PubMed38293399

2024

The effects of long-term lactate and high-intensity interval training (HIIT) on brain neuroplasticity of aged mice.

Applications

Unspecified application

Species

Unspecified reactive species

Zhou Lei,Soroosh Mozaffaritabar,Takuji Kawamura,Atsuko Koike,Attila Kolonics,Johanna Kéringer,Ricardo A Pinho,Jingquan Sun,Ruonan Shangguan,Zsolt Radák
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