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Pyruvate Assay Kit (Colorimetric/Fluorometric) ab65342 provides a simple, direct and automation-ready procedure for measuring pyruvate concentration in various biological samples such as blood, cells, culture and fermentation media, etc.
Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
Is this your first time purchasing this assay kit? Get 20% off one kit by entering code 20OFF-T5A8Q at checkout. T&C’s apply – click here. Offer only valid when purchasing directly from Abcam in Europe, North America, Australia, and Singapore.
- Detection method
Colorimetric/Fluorometric
- Sample types
Urine, Plasma, Cell culture extracts, Tissue Extracts, Serum, Other biological fluids
- Assay type
Quantitative
- Range
1 - 200 µM
- Assay time
40m
- Sensitivity
> 1 µM
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Pyruvate Assay Kit (Colorimetric/Fluorometric) ab65342 provides a simple, direct and automation-ready procedure for measuring pyruvate concentration in various biological samples such as blood, cells, culture and fermentation media, etc.
Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
Is this your first time purchasing this assay kit? Get 20% off one kit by entering code 20OFF-T5A8Q at checkout. T&C’s apply – click here. Offer only valid when purchasing directly from Abcam in Europe, North America, Australia, and Singapore.
Key facts
- Detection method
Colorimetric/Fluorometric
- Sample types
Urine, Plasma, Cell culture extracts, Tissue Extracts, Serum, Other biological fluids
- Assay type
Quantitative
- Range
1 - 200 µM
- Assay time
40m
- Assay Platform
Microplate reader
- Sensitivity
> 1 µM
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
-20°C
- Appropriate long-term storage conditions
-20°C
- Storage information
-20°C
Notes
Pyruvate Assay Kit (Colorimetric/Fluorometric) ab65342 provides a simple, direct and automation-ready procedure for measuring pyruvate concentration in various biological samples such as blood, cells, culture and fermentation media, etc.
In the pyruvate assay protocol, pyruvate is oxidized by pyruvate oxidase via enzyme reactions to generate color (λ= 570 nm) and fluorescence (at Ex/Em = 535/587 nm). Since the color or fluorescence intensity is proportional to pyruvate content, the pyruvate concentration can be accurately measured.
The kit detects 1-200 μM pyruvate.
Pyruvate assay protocol summary:
- add samples and standards to wells
- add reaction mix and incubate for 30 min at room temp
- analyze with microplate reader
This product is manufactured by BioVision, an Abcam company and was previously called K609 Pyruvate Colorimetric/Fluorometric Assay Kit. K609-100 is the same size as the 100 test size of ab65342.
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