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Pyruvate Assay Kit (Colorimetric/Fluorometric) ab65342 provides a simple, direct and automation-ready procedure for measuring pyruvate concentration in various biological samples such as blood, cells, culture and fermentation media. Readout on any colorimetric (570 nm) or fluorometric (Ex/Em = 535/587 nm) plate reader.

- Complete kit including standard curve for quantitation
- Cited in >75 publications
- Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.


Images

Functional Studies - Pyruvate Assay Kit (AB65342), expandable thumbnail
  • Functional Studies - Pyruvate Assay Kit (AB65342), expandable thumbnail
  • Functional Studies - Pyruvate Assay Kit (AB65342), expandable thumbnail
  • Functional Studies - Pyruvate Assay Kit (AB65342), expandable thumbnail
  • Functional Studies - Pyruvate Assay Kit (AB65342), expandable thumbnail

Publications

Key facts

Detection method
Colorimetric/Fluorometric
Sample types
Urine, Plasma, Cell culture extracts, Tissue Extracts, Serum, Other biological fluids
Assay type
Quantitative
Range
1 - 200 µM
Assay time
40m
Sensitivity
> 1 µM

What's included?

2000 Test
Components
Assay Buffer 4
20 x 25 mL
Developer Mix A
20 x 1 Vial
OxiRed™ Probe
20 x 0.2 mL
Pyruvate Standard
20 x 100 µL

Recommended products

Pyruvate Assay Kit (Colorimetric/Fluorometric) ab65342 provides a simple, direct and automation-ready procedure for measuring pyruvate concentration in various biological samples such as blood, cells, culture and fermentation media. Readout on any colorimetric (570 nm) or fluorometric (Ex/Em = 535/587 nm) plate reader.

- Complete kit including standard curve for quantitation
- Cited in >75 publications
- Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.

Key facts

Detection method
Colorimetric/Fluorometric
Sample types
Urine, Plasma, Cell culture extracts, Tissue Extracts, Serum, Other biological fluids
Assay type
Quantitative
Range
1 - 200 µM
Assay time
40m
Assay Platform
Microplate reader
Sensitivity
> 1 µM

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C

Notes

Pyruvate Assay Kit (Colorimetric/Fluorometric) ab65342 provides a simple, direct and automation-ready procedure for measuring pyruvate concentration in various biological samples such as blood, cells, culture and fermentation media, etc.

In the pyruvate assay protocol, pyruvate is oxidized by pyruvate oxidase via enzyme reactions to generate color (λ= 570 nm) and fluorescence (at Ex/Em = 535/587 nm). Since the color or fluorescence intensity is proportional to pyruvate content, the pyruvate concentration can be accurately measured.

The kit detects 1-200 μM pyruvate.

Pyruvate assay protocol summary:
- add samples and standards to wells
- add reaction mix and incubate for 30 min at room temp
- analyze with microplate reader

This product is manufactured by BioVision, an Abcam company and was previously called K609 Pyruvate Colorimetric/Fluorometric Assay Kit. K609-100 is the same size as the 100 test size of ab65342.

The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Pyruvate often called pyruvic acid or 'pyr' is an important intermediate metabolite in cellular respiration. It has a molecular mass of 88.06 g/mol and plays an integral role in the metabolic process. Pyruvate is present in virtually all living cells including plants and animals. The compound forms during glycolysis through the breakdown of glucose and can undergo several conversions serving as a precursor for various biochemical pathways. Potassium pyruvate is the salt form commonly used to study pyruvate's functions in the lab.

Biological function summary

This compound serves essential roles in energy production and biosynthesis. As part of the pyruvate dehydrogenase complex pyruvate connects glycolysis to the Krebs cycle by enabling the conversion to acetyl-CoA. This conversion is necessary for the aerobic respiration process. Furthermore pyruvate contributes to gluconeogenesis where it influences the creation of glucose from non-carbohydrate sources important during periods of fasting or intense exercise.

Pathways

Pyruvate interacts significantly in metabolic pathways like glycolysis and the citric acid cycle emphasizing cellular energy metabolism. In glycolysis pyruvate acts as the endpoint molecule while in the citric acid cycle it enters as acetyl-CoA maintaining the cycle in motion. The regulation of these pathways involves various enzymes and proteins like pyruvate kinase and lactate dehydrogenase associate with pyruvate’s metabolic roles dictating transitions between aerobic and anaerobic states.

Associated diseases and disorders

Alterations in pyruvate metabolism link closely to cancer and diabetes. Cancer cells often exhibit increased glycolytic rates known as the Warburg effect where pyruvate's conversion influences tumor growth. Pyruvate kinase M2 a protein variant is notably expressed in cancer cells and affects pyruvate's fate. In diabetes impaired pyruvate metabolism impacts insulin regulation contributing to the disease's metabolic complications. High levels of pyruvate can lead to lactic acidosis emphasizing the balance required in normal metabolic functioning.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Functional Studies - Pyruvate Assay Kit (ab65342), expandable thumbnail

    Functional Studies - Pyruvate Assay Kit (ab65342)

    Pyruvate measured fluorometrically in biological fluids showing quantity (nmol) per mL of tested sample

  • Functional Studies - Pyruvate Assay Kit (ab65342), expandable thumbnail
    Kaur, Gursharan et al., Scientific reports vol. 7,1 11645., Fig 2, doi:10.1038/s41598-017-12004-3

    Functional Studies - Pyruvate Assay Kit (ab65342)

    Pyruvate levels under manganese stress and iron supplementation were measured with ab65342 and the results were plotted as a function of normalized absorbance at 450 nm per milligram of cellular proteins.

  • Functional Studies - Pyruvate Assay Kit (ab65342), expandable thumbnail

    Functional Studies - Pyruvate Assay Kit (ab65342)

    Pyruvate measured fluorometrically in biological fluids showing quantity (nmol) per mln of tested cells

  • Functional Studies - Pyruvate Assay Kit (ab65342), expandable thumbnail

    Functional Studies - Pyruvate Assay Kit (ab65342)

    Pyruvate measured colourimetrically in biological fluids showing quantity (nmol) per mL of tested sample

  • Functional Studies - Pyruvate Assay Kit (ab65342), expandable thumbnail

    Functional Studies - Pyruvate Assay Kit (ab65342)

    Pyruvate colorimetric standard curve.

  • Functional Studies - Pyruvate Assay Kit (ab65342), expandable thumbnail

    Functional Studies - Pyruvate Assay Kit (ab65342)

    Pyruvate fluorometric standard curve.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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