Pyruvate Dehydrogenase Activity Assay Kit

Product details

<p>Pyruvate Dehydrogenase Activity Assay Kit ab287837 provides a quick and easy way to monitor PDH activity. In the assay, PDH converts pyruvate into an intermediate, which reduces the developer to a colored product with strong absorbance at 450 nm. The assay is simple, sensitive and can detect pyruvate dehydrogenase activity lower than 0.1 mU in a variety of samples.</p>

<p><b>Other Notes</b><br>
This product is manufactured by BioVision, an Abcam company and was previously called K679 Pyruvate Dehydrogenase (PDH) Activity Colorimetric Assay Kit. K679-100 is the same size as the 96 test size of ab287837.</p>

<p>Pyruvate Dehydrogenase (PDH) has a vital role in carbohydrate metabolism. It forms a well-characterized enzyme complex with dihydrolipoyl transacetylase (E2) and dihydrolipoyl dehydrogenase (E3). PDH converts pyruvate into acetyl-CoA in the presence of NAD and CoA, and links glycolysis to the citric acid cycle. PDH activity is inhibited by high intracellular ratios of ATP/ADP, NADH/NAD or Acetyl-CoA/CoA. In humans, PDH deficiency reduces mitochondrial function and is linked to neurodegenerative diseases. PDH deficiency is X-linked; it results in 2 forms of abnormality: a metabolic form (lactic acidosis) and a neurological form (seizure and/or neuropathological spasm). Recent studies show that PDH is a target of oncogene-induced senescence; activation of PDH enhances pyruvate utilization and increases respiration and redox stress.</p>

The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.

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