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Pyruvate dehydrogenase (PDH) Enzyme Activity Microplate Assay Kit (ab109902) can be used to determine the activity of PDH in a human, bovine, mouse, or rat sample.

Detection method

Colorimetric

Sample types

Tissue Lysate, Cell culture extracts, Purified mitochondria

Assay type

Enzyme activity

Reactive species

Mouse, Rat, Cow, Human

Assay time

3h 30m

Images

What's included?

96 Test
Components
20X Buffer
1 x 15 mL
20X Reagent Mix
2 x 600 µL
5X Stabilizer
1 x 13 mL
96-well Microplate (12 strips)
1 x 1 Unit
Coupler
1 x 250 µL
Detergent
2 x 1 mL
Reagent Dye
1 x 250 µL

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Pyruvate dehydrogenase (PDH) Enzyme Activity Microplate Assay Kit (ab109902) can be used to determine the activity of PDH in a human, bovine, mouse, or rat sample.

Key facts

Detection method

Colorimetric

Sample types

Tissue Lysate, Cell culture extracts, Purified mitochondria

Assay type

Enzyme activity

Reactive species

Mouse, Rat, Cow, Human

Assay time

3h 30m

Assay Platform

Microplate reader

Storage

Shipped at conditions

Dry Ice

Appropriate long-term storage conditions

Multi

Storage information

Please refer to protocols

Notes

Pyruvate dehydrogenase (PDH) Enzyme Activity Microplate Assay Kit (ab109902) can be used to determine the activity of PDH in a human, bovine, mouse, or rat sample. The PDH enzyme is immunocaptured within the wells of the microplate and activity is determined by following the reduction of NAD+ to NADH, coupled to the reduction of a reporter dye to yield a colored reaction product with an increase in absorbance at 450 nm at room temperature. Included for performance of the activity assay are buffer, detergent, reagent mix, and a 96-well microplate with monoclonal antibody pre-bound to the wells of the plate, allowing for a stream-lined assay.

This assay is optimized for use with whole tissue extract when the amount of total material available for assay is 20-100 μg or more. If using cell extract of cultured cells 1 mg of material is required due to the very low levels of enzyme and reduced levels of mitochondria in the extract.

The PDH complex is relatively fragile and sensitive to detergent. Please follow the sample preparation steps provided in the protocol booklet. Other preparation methods may decrease PDH activity. Our Scientific Support team is happy to answer any questions about sample prep.

Review our Metabolism Assay Guide to learn about assays for metabolites, metabolic enzymes, mitochondrial function, and oxidative stress, and also about how to assay metabolic function in live cells using your plate reader.

Store 20X Buffer, Detergent, and Microplate at 4°C.

Store 5X Stabilizer, 20X Reagent Mix, 100X Reagent Dye and 100X Coupler at -80°C.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

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Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES'.

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