Pyruvate Kinase Assay Kit

Pyruvate Kinase Assay Kit ab83432 provides a simple, direct and automation-ready assay for measuring pyruvate kinase activity in cell culture supernatant, plasma, serum, tissue, urine and other biological fluids (not validated for use with whole blood).

In the pyruvate kinase assay protocol, PEP and ADP are catalyzed by pyruvate kinase to generate pyruvate and ATP. The generated pyruvate is oxidized by pyruvate oxidase to produce color (absorbance 570 nm) and fluorescence (Ex/Em 535/587 nm). Since the increase in color or fluorescence intensity is proportional to the increase in pyruvate amount, the PK activity can be accurately measured.

The kit detects 0.1 mU/ml pyruvate kinase.

Pyruvate kinase assay protocol summary:
- add samples and standards to wells
- add reaction mix
- analyze with microplate reader immediately and after 10-20 min

This product is manufactured by BioVision, an Abcam company and was previously called K709 Pyruvate Kinase Activity Colorimetric/Fluorometric Assay Kit. K709-100 is the same size as the 100 test size of ab83432.

Pyruvate kinase (PK, EC 2.7.1.40) is an enzyme involved in glycolysis. It catalyzes the transfer of a phosphate group from phosphoenolpyruvate (PEP) to ADP, yielding one molecule of pyruvate and one molecule of ATP. Lack of pyruvate kinase will slow down the process of glycolysis which causes the disease known as pyruvate kinase deficiency.