Rac2 Activation Assay Kit

Product details

Rac2 Activation Assay Kit (ab211162) uses PAK1 PBD Agarose beads to selectively isolate and pull-down the active form of Rac from purified samples or endogenous lysates. Subsequently, the precipitated GTP-Rac is detected by western blot analysis using an anti-Rac2 specific polyclonal antibody, which reacts with the human and mouse protein.

Features: 1) non radioactive assay format; 2) fast results: 1 hour assay plus electrophoresis/blotting time; 3) includes Cdc42 positive control; 4) pink colored agarose beads for easy identification during washing and aspiration steps.

Small GTP-binding proteins (or GTPases) are a family of proteins that serve as molecular regulators in signaling transduction pathways.

Rac2 (Ras-Related C3 Botulinum Toxin Substrate 2), a 21 kDa protein, belongs to the family of Rho GTPases and regulates a variety of biological response pathways that include secretory processes, phagocytose of apoptotic cells and epithelial cell polarization. Like other small GTPases, Rac2 regulates molecular events by cycling between an inactive GDP-bound form and an active GTP-bound form. In its active (GTP-bound) state, Rac2 binds specifically to the p21-binding domain (PBD) of p21-activated protein kinase (PAK) to control downstream signaling cascades.

Diseases associated with Rac2 include neutrophil immunodeficiency syndrome and chronic granulomatous disease.

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