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AB138875

Renin Assay Kit (Fluorometric)

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(4 Publications)

Renin Assay Kit (Fluorometric) (ab138875) provides a convenient assay for high throughput screening of renin inhibitors and for continuous assay of renin activity using a TF3/TQ3 FRET peptide.

View Alternative Names

Renin, Angiotensinogenase, REN

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Functional Studies - Renin Assay Kit (Fluorometric) (AB138875)
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Supplier Data

Functional Studies - Renin Assay Kit (Fluorometric) (AB138875)

Sample Standard Curve for Renin. Renin dose response was measured with ab138875 in a 96-well black solid plate using a fluorescence microplate reader. As low as 10 ng /mL Renin was detected with 60 minutes incubation in 37°C

Key facts

Detection method

Fluorescent

Sample types

Cell Lysate

Assay type

Quantitative

Sensitivity

= 10 ng/mL

Assay Platform

Microplate reader

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Product details

Renin Assay Kit (Fluorometric) (ab138875) provides a convenient assay for high throughput screening of renin inhibitors and for continuous assay of renin activity using a TF3/TQ3 FRET peptide. In the FRET peptide the fluorescence of TF3 is quenched by TQ3. Upon cleavage into two separate fragments by renin, the fluorescence of TF3 is recovered, the fluorescent signal can be easily monitored by a fluorescence microplate reader at Ex/Em = 540/590 nm. This assay is about fifty fold more sensitive than an EDANS/DABCYL-based assay.
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What's included?

{ "values": { "100Test": { "sellingSize": "100 Test", "publicAssetCode":"ab138875-100Test", "assetComponentDetails": [ { "size":"1 x 10 mL", "name":"Assay Buffer", "number":"AB138875-CMP01", "productcode":"" }, { "size":"1 x 50 µL", "name":"Renin Red Substrate (100x)", "number":"AB138875-CMP02", "productcode":"" }, { "size":"1 x 25 µL", "name":"Renin Standard", "number":"AB138875-CMP03", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Renin also known as angiotensinogenase is a proteolytic enzyme with a molecular weight of approximately 40 kDa. It is mainly expressed in the juxtaglomerular cells of the kidneys. Renin plays an important role in the renin-angiotensin system where it initiates the conversion of angiotensinogen to angiotensin I. This process is the first step in a cascade leading to a series of effects in blood pressure regulation and fluid balance.
Biological function summary

The renin enzyme is responsible for blood pressure control and electrolyte homeostasis. It acts as a significant regulator within the renin-angiotensin aldosterone system (RAAS). By catalyzing the formation of angiotensin I from angiotensinogen renin sets off a sequence of reactions that influence vascular tone and sodium reabsorption. The activity of renin significantly interacts with various cardiovascular functions alongside complex regulatory feedback from other hormones such as aldosterone.

Pathways

Renin is a pivotal part of RAAS and interlinks with multiple cardiovascular pathways. The RAAS pathway involves angiotensinogen renin angiotensin I converting enzyme (ACE) and angiotensin II. Angiotensin II then acts on its receptor to mediate vasoconstriction and aldosterone secretion. The direct relationship between renin and ACE places this enzyme in the center of blood pressure management and has implications on kidney function and homeostasis.

High renin activity has connections with hypertension and cardiovascular diseases. Elevated renin levels contribute to increased angiotensin II production causing vasoconstriction and blood pressure elevation. Disorders like renal hypertension see direct involvement of the renin-angiotensin system. Therapeutic approaches often target RAAS with angiotensin-converting enzyme inhibitors (ACE inhibitors) and angiotensin receptor blockers aiming to mitigate the renin-related impact on these diseases.
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Product protocols

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Target data

Renin is a highly specific endopeptidase, whose only known function is to generate angiotensin I from angiotensinogen in the plasma, initiating a cascade of reactions that produce an elevation of blood pressure and increased sodium retention by the kidney.
See full target information REN
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

PloS one 17:e0267938 PubMed35862303

2022

A cardiac-null mutation of Prdm16 causes hypotension in mice with cardiac hypertrophy via increased nitric oxide synthase 1.

Applications

Unspecified application

Species

Unspecified reactive species

Ji-One Kang,Tae Woong Ha,Hae-Un Jung,Ji Eun Lim,Bermseok Oh

JCI insight 5: PubMed33170807

2020

Depletion of protein kinase STK25 ameliorates renal lipotoxicity and protects against diabetic kidney disease.

Applications

Unspecified application

Species

Unspecified reactive species

Emmelie Cansby,Mara Caputo,Lei Gao,Nagaraj M Kulkarni,Annika Nerstedt,Marcus Ståhlman,Jan Borén,Rando Porosk,Ursel Soomets,Matteo Pedrelli,Paolo Parini,Hanns-Ulrich Marschall,Jenny Nyström,Brian W Howell,Margit Mahlapuu

EBioMedicine 36:329-346 PubMed30322799

2018

Impacts of Diabetes and an SGLT2 Inhibitor on the Glomerular Number and Volume in db/db Mice, as Estimated by Synchrotron Radiation Micro-CT at SPring-8.

Applications

Unspecified application

Species

Unspecified reactive species

Yumi Takiyama,Toshihiro Sera,Masanori Nakamura,Kanaki Ishizeki,Yasuaki Saijo,Tsuyoshi Yanagimachi,Manami Maeda,Ryoichi Bessho,Takao Takiyama,Hiroya Kitsunai,Hidemitsu Sakagami,Daisuke Fujishiro,Yukihiro Fujita,Yuichi Makino,Atsuko Abiko,Masato Hoshino,Kentaro Uesugi,Naoto Yagi,Tsuguhito Ota,Masakazu Haneda

Nature communications 6:8835 PubMed26617239

2015

Endothelial Gata5 transcription factor regulates blood pressure.

Applications

FuncS

Species

Mouse

Smail Messaoudi,Ying He,Alex Gutsol,Andrew Wight,Richard L Hébert,Ragnar O Vilmundarson,Andrew P Makrigiannis,John Chalmers,Pavel Hamet,Johanne Tremblay,Ruth McPherson,Alexandre F R Stewart,Rhian M Touyz,Mona Nemer
View all publications
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