Renin Assay Kit (Fluorometric) (ab138875) provides a convenient assay for high throughput screening of renin inhibitors and for continuous assay of renin activity using a TF3/TQ3 FRET peptide.
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- PloS one 17:e02679382022A cardiac-null mutation of Prdm16 causes hypotension in mice with cardiac hypertrophy via increased nitric oxide synthase 1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJi-One Kang,Tae Woong Ha,Hae-Un Jung,Ji Eun Lim,Bermseok OhPubMed 35862303
- JCI insight 5:2020Depletion of protein kinase STK25 ameliorates renal lipotoxicity and protects against diabetic kidney disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEmmelie Cansby et. al.PubMed 33170807
- EBioMedicine 36:329-3462018Impacts of Diabetes and an SGLT2 Inhibitor on the Glomerular Number and Volume in db/db Mice, as Estimated by Synchrotron Radiation Micro-CT at SPring-8.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYumi Takiyama et. al.PubMed 30322799
- Nature communications 6:88352015Endothelial Gata5 transcription factor regulates blood pressure.
Key facts
- Detection method
- Fluorescent
- Sample types
- Cell Lysate
- Assay type
- Quantitative
- Reactive species
- Mammals
- Sensitivity
- = 10 ng/mL
Target data
Function
Renin is a highly specific endopeptidase, whose only known function is to generate angiotensin I from angiotensinogen in the plasma, initiating a cascade of reactions that produce an elevation of blood pressure and increased sodium retention by the kidney.
Alternative names
Renin, Angiotensinogenase, REN
What's included?
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Renin Assay Kit (Fluorometric) (ab138875) provides a convenient assay for high throughput screening of renin inhibitors and for continuous assay of renin activity using a TF3/TQ3 FRET peptide.
Key facts
- Detection method
- Fluorescent
- Sample types
- Cell Lysate
- Assay type
- Quantitative
- Reactive species
- Mammals
- Assay Platform
- Microplate reader
- Sensitivity
- = 10 ng/mL
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
Renin Assay Kit (Fluorometric) (ab138875) provides a convenient assay for high throughput screening of renin inhibitors and for continuous assay of renin activity using a TF3/TQ3 FRET peptide. In the FRET peptide the fluorescence of TF3 is quenched by TQ3. Upon cleavage into two separate fragments by renin, the fluorescence of TF3 is recovered, the fluorescent signal can be easily monitored by a fluorescence microplate reader at Ex/Em = 540/590 nm. This assay is about fifty fold more sensitive than an EDANS/DABCYL-based assay.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Renin also known as angiotensinogenase is a proteolytic enzyme with a molecular weight of approximately 40 kDa. It is mainly expressed in the juxtaglomerular cells of the kidneys. Renin plays an important role in the renin-angiotensin system where it initiates the conversion of angiotensinogen to angiotensin I. This process is the first step in a cascade leading to a series of effects in blood pressure regulation and fluid balance.
- Biological function summary
The renin enzyme is responsible for blood pressure control and electrolyte homeostasis. It acts as a significant regulator within the renin-angiotensin aldosterone system (RAAS). By catalyzing the formation of angiotensin I from angiotensinogen renin sets off a sequence of reactions that influence vascular tone and sodium reabsorption. The activity of renin significantly interacts with various cardiovascular functions alongside complex regulatory feedback from other hormones such as aldosterone.
- Pathways
Renin is a pivotal part of RAAS and interlinks with multiple cardiovascular pathways. The RAAS pathway involves angiotensinogen renin angiotensin I converting enzyme (ACE) and angiotensin II. Angiotensin II then acts on its receptor to mediate vasoconstriction and aldosterone secretion. The direct relationship between renin and ACE places this enzyme in the center of blood pressure management and has implications on kidney function and homeostasis.
- Associated diseases and disorders
High renin activity has connections with hypertension and cardiovascular diseases. Elevated renin levels contribute to increased angiotensin II production causing vasoconstriction and blood pressure elevation. Disorders like renal hypertension see direct involvement of the renin-angiotensin system. Therapeutic approaches often target RAAS with angiotensin-converting enzyme inhibitors (ACE inhibitors) and angiotensin receptor blockers aiming to mitigate the renin-related impact on these diseases.
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Functional Studies - Renin Assay Kit (Fluorometric) (ab138875)
Sample Standard Curve for Renin. Renin dose response was measured with ab138875 in a 96-well black solid plate using a fluorescence microplate reader. As low as 10 ng /mL Renin was detected with 60 minutes incubation in 37°C
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