Resazurin Assay Kit - Cell Cytotoxicity (Fluorometric)
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Functional Studies - Resazurin Assay Kit - Cell Cytotoxicity (Fluorometric) (AB112119)
CHO-K1 cell number response was measured with ab112119. CHO-K1 cells at 0 to 10,000 cells/well/100 µL were seeded overnight in a black wall/clear bottom 96-well plate. The cells were incubated with 20 µL/well of Assay Solution for 3 hours at 37 °C. The absorbance intensity was measured at 570 nm and 605 nm. The fluorescence intensity was linear (R2 = 0.998) to the cell number as indicated. The detection limit from the blank controls was 60 cells/well (n=6).
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Recommended alternative kit
We recommend that you purchase Resazurin Assay Kit ab129732 instead of this kit.
Resazurin Assay Kit ab112119 (Cell Cytotoxicity, Fluorometric) ab112119 provides a fast, simple, accurate and homogeneous assay for the fluorometric detection of viable cells.
The resazurin assay protocol is based on the reduction of oxidized non-fluorescent blue resazurin to a red fluorescent dye (resorufin) by the mitochondrial respiratory chain in live cells. The amount of resorufin produced is directly proportional to the number of living cells. Resorufin has Ex/Em of 530-560 / 590 nm.
The resazurin assay is very commonly used to quantify the numbers of live cells, and monitor cell viability / cytotoxicity.
ab112119 is more sensitive for cell proliferation and cytotoxicity than other assays such as MTT. The kit components are stable and have minimal cytotoxicity, thus a longer incubation time (such as 24-48 hours) is possible if required.
Resazurin assay protocol summary:
- add assay solution to cells in 96 or 384 well plates and shake gently for 30 sec
- incubate for 1-24 hr at 37°C
- analyze with microplate reader
ab112119 should be stored desiccated
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Publications (3)
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The Journal of experimental medicine 216:2057-2070 PubMed31270247
2019
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The Journal of allergy and clinical immunology 139:1995-1997.e9 PubMed28087227
2017
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Molecular therapy : the journal of the American Society of Gene Therapy 24:488-98 PubMed26581162
2015
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