Resazurin Assay Kit (Cell Viability)

6 product images

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  Functional Studies - Resazurin Assay Kit (Cell Viability) (ab129732)

  Jurkat cells were seeded at 25,000 cells per well in a 50 μL volume and immediately overlayed with a 2X concentration of the indicated drug for a final volume of 100 μL. Cells were incubated for 2 hours with the indicated drug prior to the addition of 2X stain diluted in RPMI media. After 4 hours of further incubation with stain, fluorescence was measured. IC50 for the indicated drug was found at 22 – 25 μM.

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  Functional Studies - Resazurin Assay Kit (Cell Viability) (ab129732)

  Jurkat cells were seeded at 25,000 cells per well in a 50 µL volume and immediately overlayed with a 2X concentration of Staurosporin for a final volume of 100 µL. Cells were incubated for 4 hours with Staurosporin prior to the addition of 2X stain diluted in RPMI media. After 4 hours of further incubation with stain, fluorescence was measured. IC50 for staurosporin was found at 500 nM.

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  Functional Studies - Resazurin Assay Kit (Cell Viability) (ab129732)

  Dynamic range and suitability of the mitochondrial viability assay for high throughput screening. Cells were seeded in a titration series with n=11 for each data point. Colorimetric readout gave on average a Z factor of 0.72 on HepG2 cells seeded from 40,000 – 150,000 cells per well.

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  Functional Studies - Resazurin Assay Kit (Cell Viability) (ab129732)

  Dynamic range and suitability of the mitochondrial viability assay for high throughput screening. Cells were seeded in a titration series with n=11 for each data point. Fluorometric readout gave on average a Z factor of 0.82 on Jurkat cells seeded from 1,500 – 1000,000 cells per well on a 96-well plate.

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  Functional Studies - Resazurin Assay Kit (Cell Viability) (ab129732)

  Mitochondrial viability stain in long term toxicity. HepG2 cells previously cultured in glucose or galactose substrate media were seeded at 10,000 cells per well and allowed to adhere overnight. Media was replaced for the specific culture media in the presence of a titration series of Rotenone (5 µM – 0.5 pM). Cells were treated for 72 hours prior to the addition of 2X stain. After 4 hours of incubation plates were analyzed by fluorescent readout.

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  Functional Studies - Resazurin Assay Kit (Cell Viability) (ab129732)

  Effect of stain incubation time on assay reproducibility and background. Dark wall plates were seeded with a titration series of each cell line in a final volume of 100 µL of media. 2X stain (diluted in the cell line specific growth media) was added to each well at specified time points. XY graphs data is shown after media and background substraction. Bar graph shows mean background staining at different time points. 4 hour incubation of stain results in optimal reproducibility with minimal increase of background over the 2 hour incubation.