Skip to main content

Resazurin Assay Kit ab129732 is a fluorometric/colorimetric assay used to measure the metabolic capacity of live cells.

Is this your first time purchasing this assay kit? Get 20% off one kit by entering code 20OFF-T5A8Q at checkout. T&C’s apply – click here. Offer only valid when purchasing directly from Abcam in Europe, North America, Australia, and Singapore.

Be the first to review this product! Submit a review

Images

Key facts

Detection method

Fluorescent

Sample types

Suspension cells, Adherent cells

Assay type

Cell-based (quantitative)

Loading...
Loading...
Loading...
Loading...
Loading...
Loading...
Loading...
Loading...

Alternative names

What's included?

2000 Test
Components
20X Cell Viability Stain
1 x 24 mL

Recommended products

  1. Loading...
  2. Loading...
  3. Loading...
  4. Loading...

Resazurin Assay Kit ab129732 is a fluorometric/colorimetric assay used to measure the metabolic capacity of live cells.

Is this your first time purchasing this assay kit? Get 20% off one kit by entering code 20OFF-T5A8Q at checkout. T&C’s apply – click here. Offer only valid when purchasing directly from Abcam in Europe, North America, Australia, and Singapore.

Alternative names

Key facts

Detection method

Fluorescent

Sample types

Suspension cells, Adherent cells

Assay type

Cell-based (quantitative)

Assay Platform

Microplate reader

Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

Notes

Resazurin Assay Kit ab129732 is a fluorometric/colorimetric assay used to measure the metabolic capacity of live cells.

The resazurin assay is commonly used to quantify the number of live cells in a sample, and to monitor cell viability / cytotoxicity. It is a fast, simple, accurate and homogeneous / no-wash high throughput assay that can be used to monitor cells for up to 24-48 hours.

The resazurin assay protocol is based on the reduction of oxidized non-fluorescent blue resazurin to a red fluorescent dye (resorufin) by the mitochondrial respiratory chain in live cells. The amount of resorufin produced is directly proportional to the number of living cells. Resorufin has Ex/Em of 530-560 / 590 nm and absorbance at absorbance at 570nm.

The resazurin dye can be measured in fluorescence or absorbance mode. However fluorescence mode measurement offers greater assay linearity, reproducibility, robustness and sensitivity.

Resazurin assay protocol summary:
- add resazurin reagent to cells in growth media and incubate for 4 hr at 37°C
- analyze with microplate reader

This kit was previously called Mitochondrial Viability Stain.Review our to learn about our kits to perform a , or .

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Functional Studies - Resazurin Assay Kit (Cell Viability) (ab129732), expandable thumbnail

    Functional Studies - Resazurin Assay Kit (Cell Viability) (ab129732)

    Jurkat cells were seeded at 25,000 cells per well in a 50 µL volume and immediately overlayed with a 2X concentration of Staurosporin for a final volume of 100 µL. Cells were incubated for 4 hours with Staurosporin prior to the addition of 2X stain diluted in RPMI media. After 4 hours of further incubation with stain, fluorescence was measured. IC50 for staurosporin was found at 500 nM.

  • Functional Studies - Resazurin Assay Kit (Cell Viability) (ab129732), expandable thumbnail

    Functional Studies - Resazurin Assay Kit (Cell Viability) (ab129732)

    Jurkat cells were seeded at 25,000 cells per well in a 50 μL volume and immediately overlayed with a 2X concentration of the indicated drug for a final volume of 100 μL. Cells were incubated for 2 hours with the indicated drug prior to the addition of 2X stain diluted in RPMI media. After 4 hours of further incubation with stain, fluorescence was measured. IC50 for the indicated drug was found at 22 – 25 μM.

  • Functional Studies - Resazurin Assay Kit (Cell Viability) (ab129732), expandable thumbnail

    Functional Studies - Resazurin Assay Kit (Cell Viability) (ab129732)

    Dynamic range and suitability of the mitochondrial viability assay for high throughput screening. Cells were seeded in a titration series with n=11 for each data point. Colorimetric readout gave on average a Z factor of 0.72 on HepG2 cells seeded from 40,000 – 150,000 cells per well.

  • Functional Studies - Resazurin Assay Kit (Cell Viability) (ab129732), expandable thumbnail

    Functional Studies - Resazurin Assay Kit (Cell Viability) (ab129732)

    Dynamic range and suitability of the mitochondrial viability assay for high throughput screening. Cells were seeded in a titration series with n=11 for each data point. Fluorometric readout gave on average a Z factor of 0.82 on Jurkat cells seeded from 1,500 – 1000,000 cells per well on a 96-well plate.

  • Functional Studies - Resazurin Assay Kit (Cell Viability) (ab129732), expandable thumbnail

    Functional Studies - Resazurin Assay Kit (Cell Viability) (ab129732)

    Mitochondrial viability stain in long term toxicity. HepG2 cells previously cultured in glucose or galactose substrate media were seeded at 10,000 cells per well and allowed to adhere overnight. Media was replaced for the specific culture media in the presence of a titration series of Rotenone (5 µM – 0.5 pM). Cells were treated for 72 hours prior to the addition of 2X stain. After 4 hours of incubation plates were analyzed by fluorescent readout.

  • Functional Studies - Resazurin Assay Kit (Cell Viability) (ab129732), expandable thumbnail

    Functional Studies - Resazurin Assay Kit (Cell Viability) (ab129732)

    Effect of stain incubation time on assay reproducibility and background. Dark wall plates were seeded with a titration series of each cell line in a final volume of 100 µL of media. 2X stain (diluted in the cell line specific growth media) was added to each well at specified time points. XY graphs data is shown after media and background substraction. Bar graph shows mean background staining at different time points. 4 hour incubation of stain results in optimal reproducibility with minimal increase of background over the 2 hour incubation.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

There was a problem

We can’t download that datasheet. Please try again. If you need help, contact our Customer Services team at technical@abcam.com