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AB273299

RNase Activity Assay Kit (Fluorometric)

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(2 Publications)

RNase Activity Assay Kit (Fluorometric) (ab273299) enables researchers to measure RNase activity in buffers, reagents, and other components as well as quantitatively evaluate RNAse activity of recombinant enzymes in real time.

Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
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Functional Studies - RNase Activity Assay Kit (Fluorometric) (AB273299)
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Supplier Data

Functional Studies - RNase Activity Assay Kit (Fluorometric) (AB273299)

Fluorescence Standard Curve.

Typical standard curve – data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.

Functional Studies - RNase Activity Assay Kit (Fluorometric) (AB273299)
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Supplier Data

Functional Studies - RNase Activity Assay Kit (Fluorometric) (AB273299)

Sample Kinetic Curves.

Sample kinetic curve obtained from Negative Control (Blank), bacterial culture, RNase contaminated BSA and purified RNase.

Key facts

Detection method

Fluorescent

Sample types

Other biological fluids

Assay type

Enzyme activity

Results type

Quantitative

Sensitivity

= 0.4 pg/well

Assay Platform

Microplate

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Primary Antibodies

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Primary Antibodies

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  • 5
  • 50
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Enzyme activity assay": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

RNase Activity Assay Kit (Fluorometric) (ab273299) enables researchers to measure RNase activity in buffers, reagents, and other components as well as quantitatively evaluate RNAse activity of recombinant enzymes in real time.

The assay uses a highly sensitive, specific probe that releases a fluorescent product in the presence of active RNAse. The limit of detection is 0.4 pg RNase/well and limit of quantification is 1.2 pg RNase/well.

This product is manufactured by BioVision, an Abcam company and was previously called K934 RNase Activity Detection/Quantification Assay Kit (Fluorometric). K934-100 is the same size as the 100 test size of ab273299.

The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.

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What's included?

{ "values": { "100Test": { "sellingSize": "100 Test", "publicAssetCode":"ab273299-100Test", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"96-Well Half Area Plate (with lid)", "number":"AB273299-CMP02", "productcode":"" }, { "size":"1 x 250 µL", "name":"RNAse Positive Control", "number":"AB273299-CMP05", "productcode":"" }, { "size":"1 x 25 mL", "name":"Molecular Biology (RNA) Grade Water", "number":"AB273299-CMP04", "productcode":"" }, { "size":"1 x 1 mL", "name":"10X RNase Buffer", "number":"AB273299-CMP01", "productcode":"" }, { "size":"1 x 1 Vial", "name":"RNase Probe", "number":"AB273299-CMP06", "productcode":"" }, { "size":"1 x 100 µL", "name":"Fluorescence Standard I", "number":"AB273299-CMP03", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Product protocols

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Target data

See full target information RNase
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Rapid communications in mass spectrometry : RCM 39:e10048 PubMed40255129

2025

Buffer 4-Ethylmorpholinium/Acetate: Exploring a New Alternative Buffer for Native Mass Spectrometry.

Applications

Unspecified application

Species

Unspecified reactive species

Darya Hadavi,Che Yee Ng,Yuandi Zhao,Anjusha Mathew,Ian G M Anthony,Berta Cillero-Pastor,Eva Cuypers,Tiffany Porta Siegel,Maarten Honing

ACS pharmacology & translational science 6:1945-1957 PubMed38093831

2023

Screening of Pancreatic Ribonuclease A Inhibitors from an Autodisplayed Fv-Antibody Library.

Applications

Unspecified application

Species

Unspecified reactive species

Jeong Soo Sung,Jaeyong Jung,Hyemin Hwang,Ji-Hong Bong,Min-Jung Kang,Joachim Jose,Misu Lee,Jae-Chul Pyun
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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