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RNase Activity Assay Kit (Fluorometric) (ab273299) enables researchers to measure RNase activity in buffers, reagents, and other components as well as quantitatively evaluate RNAse activity of recombinant enzymes in real time.

Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.

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Images

Functional Studies - RNase Activity Assay Kit (Fluorometric) (AB273299), expandable thumbnail
  • Functional Studies - RNase Activity Assay Kit (Fluorometric) (AB273299), expandable thumbnail

Key facts

Detection method

Fluorescent

Sample types

Other biological fluids

Assay type

Enzyme activity (quantitative)

Sensitivity

= 0.4 pg/well

Target data

What's included?

100 Test
Components
10X RNase Buffer
1 x 1 mL
96-Well Half Area Plate (with lid)
1 x 1 Unit
Fluorescence Standard I
1 x 100 µL
Molecular Biology (RNA) Grade Water
1 x 25 mL
RNAse Positive Control
1 x 250 µL
RNase Probe
1 x 1 Vial

Recommended products

RNase Activity Assay Kit (Fluorometric) (ab273299) enables researchers to measure RNase activity in buffers, reagents, and other components as well as quantitatively evaluate RNAse activity of recombinant enzymes in real time.

Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.

Key facts

Detection method

Fluorescent

Sample types

Other biological fluids

Assay type

Enzyme activity (quantitative)

Assay Platform

Microplate

Sensitivity

= 0.4 pg/well

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

-20°C

Notes

RNase Activity Assay Kit (Fluorometric) (ab273299) enables researchers to measure RNase activity in buffers, reagents, and other components as well as quantitatively evaluate RNAse activity of recombinant enzymes in real time.

The assay uses a highly sensitive, specific probe that releases a fluorescent product in the presence of active RNAse. The limit of detection is 0.4 pg RNase/well and limit of quantification is 1.2 pg RNase/well.

This product is manufactured by BioVision, an Abcam company and was previously called K934 RNase Activity Detection/Quantification Assay Kit (Fluorometric). K934-100 is the same size as the 100 test size of ab273299.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Functional Studies - RNase Activity Assay Kit (Fluorometric) (ab273299), expandable thumbnail

    Functional Studies - RNase Activity Assay Kit (Fluorometric) (ab273299)

    Fluorescence Standard Curve.

    Typical standard curve – data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.

  • Functional Studies - RNase Activity Assay Kit (Fluorometric) (ab273299), expandable thumbnail

    Functional Studies - RNase Activity Assay Kit (Fluorometric) (ab273299)

    Sample Kinetic Curves.

    Sample kinetic curve obtained from Negative Control (Blank), bacterial culture, RNase contaminated BSA and purified RNase.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com