ROS/Superoxide Detection Assay Kit (Cell-based)

ROS/Superoxide Detection Assay Kit ab139476 is designed to directly monitor real time reactive oxygen species (ROS) production in live cells using fluorescence microscopy, flow cytometry, or microplate reader.

The ROS/superoxide assay protocol is based on two fluorescent dyes: Oxidative Stress Detection Reagent (Green, Ex/Em 490/525 nm) for the detection of total ROS, and Superoxide Detection Reagent (Orange, Ex/Em 550/620 nm).

Through the combination of these two specific fluorescent probes, the kit provides a simple and specific assay for the real-time measurement of global levels of total reactive oxygen species (ROS), and of superoxides, in living cells. The kit is compatible with the major components of tissue culture media (phenol red, FBS and BSA).

ROS/superoxide assay protocol summary:
- add ROS inhibitor to negative control cells and incubate for 30 min
- add ROS/superoxide detection mix (with the addition of ROS inducer for positive control cells) and incubate for 30-60 min
- remove and discard the detection mix liquid
- wash samples and analyze with fluorescence microscope, or analyze by flow cytometry or microplate reader without washing

Reagents provided in the kit are sufficient for at least 200 tests using Fluorescent Microscopy or 50 tests using Flow Cytometry. **Related products** Review the , or the full to learn about more assays for metabolites, metabolic enzymes, mitochondrial function, and oxidative stress, and also how to assay metabolic function in live cells using your plate reader. To measure reactive oxygen species within cells, we recommend . Alternative ROS assays are available in orange (ab186028), red (ab186027), and deep red (ab186029). ab238535 is used to measure ROS in biofluids, culture supernatants and cell lysates. For assays designed to differentiate ROS, superoxides, and reactive nitrogen species: to assay ROS and superoxides use ab139476; to assay ROS, superoxides, and reactive nitrogen species use ab139473; to assay superoxides use ab219943.