Senescence Assay Kit ab228562 is designed to fluorescently detect senescence-associated Beta Galactosidase activity in cultured cells by flow cytometry.
Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
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- NPJ precision oncology 7:1362023SHP2 inhibitors maintain TGFβ signalling through SMURF2 inhibition.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXianning Lai,Sarah Kit Leng Lui,Hiu Yan Lam,Yuta Adachi,Wen Jing Sim,Natali Vasilevski,Nicola J Armstrong,Stephanie Claire Bridgeman,Nathan Michael Main,Tuan Zea Tan,Janina E E Tirnitz-Parker,Jean Paul Thiery,Hiromichi Ebi,Alan Prem Kumar,Pieter Johan Adam EichhornPubMed 38102334
- Journal of experimental orthopaedics 10:312023Successful isolation of viable stem cells from cryopreserved microfragmented human adipose tissue from patients with knee osteoarthritis - a comparative study of isolation by tissue explant culture and enzymatic digestion.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJasmin Bagge,Per Hölmich,Freja Aabæk Hammer,Jan O Nehlin,Kilian Vomstein,Lars Blønd,Lisbet Rosenkrantz Hölmich,Kristoffer Weisskirchner BarfodPubMed 36952141
- The American journal of pathology 193:11-262022Anti-Cholestatic Therapy with Obeticholic Acid Improves Short-Term Memory in Bile Duct-Ligated Mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLucy M V Gee,Ben Barron-Millar,Jack Leslie,Claire Richardson,Marco Y W Zaki,Saimir Luli,Rachel A Burgoyne,Rainie I T Cameron,Graham R Smith,John G Brain,Barbara Innes,Laura Jopson,Jessica K Dyson,Katherine R C McKay,Alexandros Pechlivanis,Elaine Holmes,Rolando Berlinguer-Palmini,Stella Victorelli,George F Mells,Richard N Sandford,Jeremy Palmer,John A Kirby,Christos Kiourtis,Joao Mokochinski,Zoe Hall,Thomas G Bird,Lee A Borthwick,Christopher M Morris,Peter S Hanson,Diana Jurk,Elizabeth A Stoll,Fiona E N LeBeau,David E J Jones,Fiona OakleyPubMed 36243043
- Biomedicines 10:2022TGFβ1 Induces Senescence and Attenuated VEGF Production in Retinal Pericytes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDragana Avramovic,Sébastien A Archaimbault,Alicia M Kemble,Sabine Gruener,Mirjana Lazendic,Peter D WestenskowPubMed 35740425
- Antioxidants (Basel, Switzerland) 11:2022Gingerenone A Induces Antiproliferation and Senescence of Breast Cancer Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTzu-Jung Yu,Jen-Yang Tang,Jun-Ping Shiau,Ming-Feng Hou,Chia-Hung Yen,Fu Ou-Yang,Chung-Yi Chen,Hsueh-Wei ChangPubMed 35326237
- International journal of molecular medicine 47:2021Apremilast prevents IL‑17‑induced cellular senescence in ATDC5 chondrocytes mediated by SIRT1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBenlong Wang et. al.PubMed 33448323
- Nature structural & molecular biology 26:899-9092019G-tract RNA removes Polycomb repressive complex 2 from genes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesManuel Beltran et. al.PubMed 31548724
- Clinical cancer research : an official journal of the American Association for Cancer Research 24:5744-57562018Mutant LKB1 Confers Enhanced Radiosensitization in Combination with Trametinib in KRAS-Mutant Non-Small Cell Lung Cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYifan Wang,Nan Li,Wen Jiang,Weiye Deng,Rui Ye,Cai Xu,Yawei Qiao,Amrish Sharma,Ming Zhang,Mien-Chie Hung,Steven H LinPubMed 30068711
Key facts
- Detection method
Fluorescent
- Sample types
Suspension cells, Adherent cells
- Assay type
Cell-based (quantitative)
Associated Products
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Target data
Function
Isoform 1Cleaves beta-linked terminal galactosyl residues from gangliosides, glycoproteins, and glycosaminoglycans.Isoform 2Has no beta-galactosidase catalytic activity, but plays functional roles in the formation of extracellular elastic fibers (elastogenesis) and in the development of connective tissue. Seems to be identical to the elastin-binding protein (EBP), a major component of the non-integrin cell surface receptor expressed on fibroblasts, smooth muscle cells, chondroblasts, leukocytes, and certain cancer cell types. In elastin producing cells, associates with tropoelastin intracellularly and functions as a recycling molecular chaperone which facilitates the secretions of tropoelastin and its assembly into elastic fibers.
Alternative names
Beta-galactosidase, Acid beta-galactosidase, Elastin receptor 1, Lactase, GLB1, ELNR1
What's included?
Recommended products
Senescence Assay Kit ab228562 is designed to fluorescently detect senescence-associated Beta Galactosidase activity in cultured cells by flow cytometry.
Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
Alternative names
Beta-galactosidase, Acid beta-galactosidase, Elastin receptor 1, Lactase, GLB1, ELNR1
Key facts
- Detection method
Fluorescent
- Sample types
Suspension cells, Adherent cells
- Assay type
Cell-based (quantitative)
- Assay Platform
Flow cytometer
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
-20°C
- Appropriate long-term storage conditions
-20°C
- Storage information
-20°C
Notes
Senescence Assay Kit (Beta Galactosidase, Fluorescence) (ab228562) is designed to fluorescently detect senescence-associated Beta Galactosidase activity in cultured cells by flow cytometry. The senescence-associated Beta Galactosidase is present only in senescent cells and is not found in pre-senescent, quiescent or immortal cells.
Senescence Assay Kit Senescence Detection Kit ab65351 to stain for beta galactosidase activity in tissues and cell cultures with detection by microscopy.
This product is manufactured by BioVision, an Abcam company and was previously called K991 Senescence Detection Kit (Fluorometric). K991-100 is the same size as the 100 test size of ab228562.
Senescence is thought to be a tumor suppressive mechanism and an underlying cause of aging. Senescence represents an arrested state in which the cells remain viable, but not stimulated to divide by serum or passage in culture. Senescent cells display increase of cell size, senescence-associated expression of Beta Galactosidase activity, and altered patterns of gene expression.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Senescence-associated beta-galactosidase often called SA-beta-gal is an enzyme that serves as a biomarker for cellular senescence. It is a lysosomal hydrolase with a molecular mass of approximately 120 kDa. This enzyme functions mechanically by catalyzing the hydrolysis of beta-galactosides into monosaccharides. SA-beta-gal is expressed extensively in senescent cells which accumulate in various tissues as organisms age.
- Biological function summary
Senescence-associated beta-galactosidase functions as an indicator of the senescent state of cells. It does not act as part of a larger complex but is key in detecting an increase in lysosomal mass characteristic of senescence. During the aging process cells exhibit increased expression of this enzyme which scientists use in beta-galactosidase assays to identify senescent cells in culture. Fluorescence methods or beta-gal staining often facilitate visualization of senescent cells by binding to substrates processed by the enzyme.
- Pathways
The enzyme is indicative of involvement in both the p53 and p16INK4a-Rb tumor suppression pathways. Although not an active participant itself SA-beta-gal reflects altered cellular metabolism linked to these pathways often in collaboration with proteins like p53 and p16INK4a which regulate cell cycle arrest. Additionally it aligns with the decline of mitochondrial function another hallmark of senescent cells influencing cellular aging and stress response pathways.
- Associated diseases and disorders
SA-beta-gal activity correlates strongly with age-related diseases such as Alzheimer's disease and certain types of cancer. As an aged cell marker its elevated presence can signify increased cellular senescence often contributing to tissue dysfunction and promoting disease progression. In Alzheimer's disease connections to amyloid precursor protein processing and tau pathology have been observed. In cancer SA-beta-gal works alongside proteins like p53 and p16INK4a to highlight cell cycle arrest anomalies where downregulated functionality or bypass can lead to unchecked cellular proliferation.
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Flow Cytometry - Senescence Assay Kit (Beta Galactosidase, Fluorescence) (ab228562)
NIH/3T3 (mouse embryo fibroblast cell line) cells were plated at 5 x 105 cells per well in a 24 well, treated for 4 hours (with and without 200 nM of Daunorubicin HCl; test and control response respectively) in complete cell culture media for 48 hours at 37°C/5% CO2. Media was removed and replaced with media containing Senescence Dye and incubated for 2 hours at 37°C/5% CO2. After incubation time, cells were washed 2x with Wash Buffer, the cells were trypsinized, washed once with Wash Buffer and analyzed by FACS.
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