Senescence Detection Kit ab65351 is a senescence-associated beta-galactosidase staining kit / SA beta-gal staining kit. It is used to stain for SA-beta-Gal activity in cell cultures and frozen tissue sections.
- Cited in over 180 publications
- Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
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- NPJ Parkinson's disease 9:1662023Impaired neuron differentiation in GBA-associated Parkinson's disease is linked to cell cycle defects in organoids.Applications:Unspecified applicationReactive species:Unspecified reactive speciesIsabel Rosety,Alise Zagare,Claudia Saraiva,Sarah Nickels,Paul Antony,Catarina Almeida,Enrico Glaab,Rashi Halder,Sergiy Velychko,Thomas Rauen,Hans R Schöler,Silvia Bolognin,Thomas Sauter,Javier Jarazo,Rejko Krüger,Jens C SchwambornPubMed 38110400
- Scientific reports 13:220002023SIRT6 promotes metastasis and relapse in HER2-positive breast cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCristina Andreani,Caterina Bartolacci,Giuseppe Persico,Francesca Casciaro,Stefano Amatori,Mirco Fanelli,Marco Giorgio,Mirco Galié,Daniele Tomassoni,Junbiao Wang,Xiaoting Zhang,Gregory Bick,Roberto Coppari,Cristina Marchini,Augusto AmiciPubMed 38081972
- Advanced biology 8:e23001402023An Ex Vivo Aorta Culture Model to Study Vascular Cellular Senescence.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYijie Yu,Shihui Bian,Yu Jiang,Bo Li,Xinggang Cui,Shu Ding,Zhiyin Dai,Rui Chen,Wei Zhong,Wei YuanPubMed 38051940
- eLife 12:2023Hypoxia-inducible factor 1 signaling drives placental aging and can provoke preterm labor.Applications:Unspecified applicationReactive species:Unspecified reactive speciesErin J Ciampa,Padraich Flahardy,Harini Srinivasan,Christopher Jacobs,Linus Tsai,S Ananth Karumanchi,Samir M ParikhPubMed 37610425
- Cancers 15:2023BRD4 Inhibition as a Strategy to Prolong the Response to Standard of Care in Estrogen Receptor-Positive Breast Cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAhmed M Elshazly,Melanie M Sinanian,Victoria Neely,Eesha Chakraborty,Muruj A Alshehri,Michael K McGrath,Hisashi Harada,Patricia V Schoenlein,David A GewirtzPubMed 37627092
- iScience 26:1071132023Restoration of mitochondrial function by polysaccharide via upregulated SOD2 in aging fibroblasts.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKayo Machihara,Shoma Oki,Yuka Maejima,Sou Kageyama,Ayumu Onda,Yurino Koseki,Yasuyuki Imai,Takushi NambaPubMed 37416477
- Nanotheranostics 7:380-3922023Peelable Microneedle Patches Deliver Fibroblast Growth Factors to Repair Skin Photoaging Damage.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGuojun Yang,Shiqi Hu,Haiyue Jiang,Ke ChengPubMed 37426882
- Life science alliance 6:2023Time-resolved proteomic analyses of senescence highlight metabolic rewiring of mitochondria.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJun Yong Kim,Ilian Atanassov,Frederik Dethloff,Lara Kroczek,Thomas LangerPubMed 37321846
- Theranostics 13:2914-29292023PCSK9 attenuates efferocytosis in endothelial cells and promotes vascular aging.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShijie Liu,Jinzi Wu,Amanda Stolarz,Huiliang Zhang,Marjan Boerma,Stephanie D Byrum,Nancy J Rusch,Zufeng DingPubMed 37284459
- JCI insight 8:2023Tubular cell senescence promotes maladaptive kidney repair and chronic kidney disease after cisplatin nephrotoxicity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSiyao Li,Man J Livingston,Zhengwei Ma,Xiaoru Hu,Lu Wen,Han-Fei Ding,Daohong Zhou,Zheng DongPubMed 36917180
Key facts
- Detection method
- Colorimetric
- Sample types
- Tissue, Adherent cells
- Assay type
- Enzyme activity
- Assay time
- 1h 10m
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Target data
Function
Isoform 1. Cleaves beta-linked terminal galactosyl residues from gangliosides, glycoproteins, and glycosaminoglycans. Isoform 2. Has no beta-galactosidase catalytic activity, but plays functional roles in the formation of extracellular elastic fibers (elastogenesis) and in the development of connective tissue. Seems to be identical to the elastin-binding protein (EBP), a major component of the non-integrin cell surface receptor expressed on fibroblasts, smooth muscle cells, chondroblasts, leukocytes, and certain cancer cell types. In elastin producing cells, associates with tropoelastin intracellularly and functions as a recycling molecular chaperone which facilitates the secretions of tropoelastin and its assembly into elastic fibers.
Alternative names
ELNR1, GLB1, Beta-galactosidase, Acid beta-galactosidase, Elastin receptor 1, Lactase
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Recommended products
Senescence Detection Kit ab65351 is a senescence-associated beta-galactosidase staining kit / SA beta-gal staining kit. It is used to stain for SA-beta-Gal activity in cell cultures and frozen tissue sections.
- Cited in over 180 publications
- Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
Key facts
- Detection method
- Colorimetric
- Sample types
- Tissue, Adherent cells
- Assay type
- Enzyme activity
- Assay time
- 1h 10m
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Please refer to protocols
Notes
Senescence Detection Kit (ab65351) is designed to histochemically detect SA-beta-Gal activity in cultured cells and tissue sections, a known characteristic of senescent cells. The SA-beta-Gal is present only in senescent cells and is not found in presenescent, quiescent or immortal cells.
See Senescence Assay Kit Senescence Assay Kit (Beta Galactosidase, Fluorescence) ab228562 to detect beta galactosidase activity in senescent cells by flow cytometry.
SA beta-gal staining principle
The SA beta-gal staining assay includes:
- optimized tissue / cell fixative for beta-gal staining
- X-GAL beta galactosidase substrate; stains cells blue when acted on by beta-gal
- citric-phosphate beta-gal staining solution with potassium ferrocyanide / ferricyanide staining supplement
Senescence assay protocol summary
- wash cells / sections with PBS
- fix with fixative solution for 10 min
- wash with PBS
- incubate in staining solution mix for 1 hr
- analyze staining with a microscope
Other notes
This product was previously called K320 Biovision Senescence Detection Kit. Abcam acquired BioVision in 2021.
Senescence is thought to be a tumor suppressive mechanism and an underlying cause of aging. Senescence represents an arrested state in which the cells remain viable, but not stimulated to divide by serum or passage in culture. Senescent cells display increase of cell size, senescence-associated expression of beta-galactosidase (SA-beta-Gal) activity, and altered patterns of gene expression.
The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Senescence-associated beta-galactosidase often called SA-beta-gal is an enzyme that serves as a biomarker for cellular senescence. It is a lysosomal hydrolase with a molecular mass of approximately 120 kDa. This enzyme functions mechanically by catalyzing the hydrolysis of beta-galactosides into monosaccharides. SA-beta-gal is expressed extensively in senescent cells which accumulate in various tissues as organisms age.
- Biological function summary
Senescence-associated beta-galactosidase functions as an indicator of the senescent state of cells. It does not act as part of a larger complex but is key in detecting an increase in lysosomal mass characteristic of senescence. During the aging process cells exhibit increased expression of this enzyme which scientists use in beta-galactosidase assays to identify senescent cells in culture. Fluorescence methods or beta-gal staining often facilitate visualization of senescent cells by binding to substrates processed by the enzyme.
- Pathways
The enzyme is indicative of involvement in both the p53 and p16INK4a-Rb tumor suppression pathways. Although not an active participant itself SA-beta-gal reflects altered cellular metabolism linked to these pathways often in collaboration with proteins like p53 and p16INK4a which regulate cell cycle arrest. Additionally it aligns with the decline of mitochondrial function another hallmark of senescent cells influencing cellular aging and stress response pathways.
- Associated diseases and disorders
SA-beta-gal activity correlates strongly with age-related diseases such as Alzheimer's disease and certain types of cancer. As an aged cell marker its elevated presence can signify increased cellular senescence often contributing to tissue dysfunction and promoting disease progression. In Alzheimer's disease connections to amyloid precursor protein processing and tau pathology have been observed. In cancer SA-beta-gal works alongside proteins like p53 and p16INK4a to highlight cell cycle arrest anomalies where downregulated functionality or bypass can lead to unchecked cellular proliferation.
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1 product image
Angelucci S et al., Proteome Sci, 8, 18, 2010 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/2.0Immunohistochemistry (Frozen sections) - Senescence Detection Kit (ab65351)
Senescence-associated beta-galactosidase staining at the 2nd(A), 4th(B), 8th(C), 12th passage in vitro expansion. Cells were plated at a density of 10,000 cells/cm2 for 24h before staining. Five representative images (100x) were taken from diverse areas of cell culture, using phase-contrast microscopy to assess the number of positive cells. Image obtained from Angelucci S et al; Proteome Sci, 2010 Mar 26;8:18
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