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SIRT1 Activity Assay Kit (Fluorometric) (ab156065) detects SIRT1 activity in lysates.

Detection method

Fluorescent

Sample types

Cell culture extracts, Tissue Extracts

Assay type

Enzyme activity

Reactive species

Mouse, Rat, Human

Assay time

1h

Images

Target data

What's included?

100 Test
Components
Developer
1 x 500 µL
Fluoro-Deacetylated Peptide (0.2 mM)
1 x 100 µL
Fluoro-Substrate Peptide (0.2 mM)
1 x 500 µL
NAD (2 mM)
1 x 500 µL
Recombinant SIRT1
1 x 500 µL
SIRT Assay Buffer
2 x 1 mL
Stop Solution
2 x 1 mL

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SIRT1 Activity Assay Kit (Fluorometric) (ab156065) detects SIRT1 activity in lysates.

Key facts

Detection method

Fluorescent

Sample types

Cell culture extracts, Tissue Extracts

Assay type

Enzyme activity

Reactive species

Mouse, Rat, Human

Assay time

1h

Assay Platform

Microplate reader

Storage

Shipped at conditions

Dry Ice

Appropriate short-term storage conditions

-80°C

Appropriate long-term storage conditions

Multi

Storage information

Please refer to protocols

Notes

SIRT1 Activity Assay Kit (Fluorometric) (ab156065) detects SIRT1 activity in lysates. Primarily, the SIRT1 Activity Assay Kit is designed for the rapid and sensitive evaluation of SIRT1 inhibitors or activators using crude SIRT1 fraction or purified SIRT1. Additionally, any cultured primary cell, cell line, or tissue homogenate can be assayed for SIRT1 activity with the SIRT1 Activity Assay Kit if the appropriate antibody directed against SIRT1 (Anti-SIRT1 antibody (ab7343)) is used for immunoprecipitation.

Abcam's SIRT1 Activity Assay Kit (Fluorometric) has been shown to detect the activity of Sirtuins, at least SIRT1 in Human or animal cell lysates or in column fractions. The assay shows good linearity of sample response. The assay may be used to follow the purification of Sirtuins or may be used to detect the presence of Sirtuins in cell lysates.

Applications for this kit include:

1. Monitoring the purification of SIRT1.

2. Screening inhibitors or activators of SIRT1.

3. Detecting the effects of pharmacological agents on SIRT1.

Histone Deacetylases (HDACs) are a class of enzymes responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4), allowing the histones to wrap the DNA more tightly.

HDAC proteins occur in four groups (class I, class IIA, class IIB, class III, class IV) based on function and DNA sequence similarity.
Classes I, IIA and IIB are considered "classical" HDACs whose activities are inhibited by trichostatin A (TSA), whereas class III is a family of NAD+-dependent proteins (sirtuins) not affected by TSA. Class IV is considered an atypical class on its own, based solely on DNA sequence similarity to the others.

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Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES'.

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