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AB156067

SIRT3 Activity Assay Kit (Fluorometric)

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(1 Review)

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(28 Publications)

SIRT3 Activity Assay Kit (Fluorometric) (ab156067) detects SIRT3 activity in purified SIRT3 or mitochondrial extract preparations.

View Alternative Names

SIR2L3, SIRT3, hSIRT3, NAD-dependent protein delactylase sirtuin-3, Regulatory protein SIR2 homolog 3, SIR2-like protein 3

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Functional Studies - SIRT3 Activity Assay Kit (Fluorometric) (AB156067)
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Supplier Data

Functional Studies - SIRT3 Activity Assay Kit (Fluorometric) (AB156067)

Dose dependency curve of recombinant SIRT3 activity

Functional Studies - SIRT3 Activity Assay Kit (Fluorometric) (AB156067)
  • FuncS

Supplier Data

Functional Studies - SIRT3 Activity Assay Kit (Fluorometric) (AB156067)

Effect of Trichostatin A and NAD on recombinant SIRT3 activity

Key facts

Detection method

Fluorescent

Sample types

Purified protein, Mitochondrial Extract

Reacts with

Human

Assay type

Enzyme activity

Assay time

40m

Assay Platform

Microplate reader

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Complementary Products

Assay Kits

AB156065

SIRT1 Activity Assay Kit (Fluorometric)

Functional Studies - SIRT1 Activity Assay Kit (Fluorometric) (AB156065)

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Primary Antibodies

AB137037

Anti-SOD2/MnSOD (acetyl K68) antibody [EPVANR2]

RabMAb|Recombinant|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOD2/MnSOD (acetyl K68) antibody [EPVANR2] (AB137037)

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  • 4
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB72230

Anti-PGC1 alpha + beta antibody

Immunocytochemistry/ Immunofluorescence - Anti-PGC1 alpha + beta antibody (AB72230)

  • 4
  • 2
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Enzyme activity assay": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

SIRT3 Activity Assay Kit (Fluorometric) (ab156067) detects SIRT3 activity in purified SIRT3 or mitochondrial extract preparations.

Primarily, the SIRT3 Activity Assay Kit (Fluorometric) is designed for the rapid and sensitive evaluation of SIRT3 inhibitors or activators using crude SIRT3 fraction (mitochondrial extracts) or purified SIRT3.

Applications for this kit include:

1. Screening inhibitors or activators of SIRT3.

2. Detecting the effects of pharmacological agents on SIRT3.

SIRT3 assay protocol summary:
- add assay buffer, substrate peptide and NAD to wells
- add developer to wells
- add enzyme sample or recombinant SIRT3 to wells
- analyze with microplate reader for 30-60 min every 1-2 min

Other Notes
Histone Deacetylases (HDACs) are a class of enzymes responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4), allowing the histones to wrap the DNA more tightly.

HDAC proteins occur in four groups (class I, class IIA, class IIB, class III, class IV) based on function and DNA sequence similarity.
Classes I, IIA and IIB are considered "classical" HDACs whose activities are inhibited by trichostatin A (TSA), whereas class III is a family of NAD+-dependent proteins (sirtuins) not affected by TSA. Class IV is considered an atypical class on its own, based solely on DNA sequence similarity to the others.

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What's included?

{ "values": { "100Test": { "sellingSize": "100 Test", "publicAssetCode":"ab156067-100Test", "assetComponentDetails": [ { "size":"1 x 100 µL", "name":"Fluoro-Deacetylated Peptide (0.2 mM)", "number":"AB156067-CMP03", "productcode":"" }, { "size":"2 x 1 mL", "name":"Stop Solution", "number":"AB156067-CMP07", "productcode":"" }, { "size":"1 x 500 µL", "name":"Developer", "number":"AB156067-CMP02", "productcode":"" }, { "size":"1 x 500 µL", "name":"NAD (2 mM)", "number":"AB156067-CMP05", "productcode":"" }, { "size":"1 x 500 µL", "name":"Fluoro-Substrate Peptide (0.2 mM)", "number":"AB156067-CMP04", "productcode":"" }, { "size":"1 x 500 µL", "name":"Recombinant SIRT3", "number":"AB156067-CMP01", "productcode":"" }, { "size":"2 x 1 mL", "name":"SIRT Assay Buffer", "number":"AB156067-CMP06", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SIRT3 also known as Sirtuin 3 and mitochondrial sirtuin is a deacetylase with a mass of roughly 44 kDa. This protein is mostly found in the mitochondria where it regulates the acetylation state of various substrates. It plays an important role in maintaining mitochondrial homeostasis by modifying protein activity. SIRT3 is evolutionarily conserved and highly expressed in tissues with high energy demand like the heart liver and brown adipose tissue.
Biological function summary

SIRT3 modulates energy homeostasis by deacetylating and regulating enzymes involved in metabolism. It is a part of the mitochondrial sirtuin family contributing to fatty acid oxidation and the antioxidant defense system. By targeting key metabolic enzymes SIRT3 directly impacts the tricarboxylic acid (TCA) cycle and oxidative phosphorylation processes. These functions highlight its role in maintaining energy efficiency and reducing reactive oxidative species.

Pathways

SIRT3 participates in vital metabolic pathways such as the TCA cycle and the urea cycle. It interacts with other proteins like MnSOD and IDH2 to enhance mitochondrial function and overall cellular metabolism. This helps control the balance between energy production and consumption integrating signals from the AMPK and NAD+-dependent pathways to coordinate responses to changes in energy availability.

SIRT3 has implications in metabolic syndrome and cancer. Reduced SIRT3 activity is associated with increased susceptibility to metabolic disturbances like obesity and type 2 diabetes. Its deficiency affects the regulation of ROS and metabolic adaptation linking it to tumorigenesis and cancer progression. As it connects with proteins like HIF-1α in hypoxic conditions SIRT3 plays a role in cellular adaptation to stress influencing disease progression.
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Product protocols

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Target data

NAD-dependent protein deacetylase (PubMed : 12186850, PubMed : 12374852, PubMed : 16788062, PubMed : 18680753, PubMed : 18794531, PubMed : 19535340, PubMed : 23283301, PubMed : 24121500, PubMed : 24252090). Activates or deactivates mitochondrial target proteins by deacetylating key lysine residues (PubMed : 12186850, PubMed : 12374852, PubMed : 16788062, PubMed : 18680753, PubMed : 18794531, PubMed : 23283301, PubMed : 24121500, PubMed : 24252090, PubMed : 38146092). Known targets include ACSS1, IDH, GDH, SOD2, PDHA1, LCAD, SDHA, MRPL12 and the ATP synthase subunit ATP5PO (PubMed : 16788062, PubMed : 18680753, PubMed : 19535340, PubMed : 24121500, PubMed : 24252090, PubMed : 38146092). Contributes to the regulation of the cellular energy metabolism (PubMed : 24252090). Important for regulating tissue-specific ATP levels (PubMed : 18794531). In response to metabolic stress, deacetylates transcription factor FOXO3 and recruits FOXO3 and mitochondrial RNA polymerase POLRMT to mtDNA to promote mtDNA transcription (PubMed : 23283301). Acts as a regulator of ceramide metabolism by mediating deacetylation of ceramide synthases CERS1, CERS2 and CERS6, thereby increasing their activity and promoting mitochondrial ceramide accumulation (By similarity). Regulates hepatic lipogenesis (By similarity). Uses NAD(+) substrate imported by SLC25A47, triggering downstream activation of PRKAA1/AMPK-alpha signaling cascade that ultimately downregulates sterol regulatory element-binding protein (SREBP) transcriptional activities and ATP-consuming lipogenesis to restore cellular energy balance (By similarity). In addition to protein deacetylase activity, also acts as a protein-lysine deacylase by recognizing other acyl groups, such as benzoyl and lactoyl, leading to protein debenzoylation and delactylation, respectively (PubMed : 39524354, PubMed : 36896611, PubMed : 37720100). Catalyzes debenzoylation of PPIF and ACLY (PubMed : 37720100). Mediates delactylation of CCNE2 and 'Lys-16' of histone H4 (H4K16la) (PubMed : 36896611, PubMed : 37720100).
See full target information SIRT3
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Publications (28)

Recent publications for all applications. Explore the full list and refine your search

Translational neurodegeneration 14:34 PubMed40597361

2025

Deficient AMPK-SENP1-Sirt3 signaling impairs mitochondrial complex I function in Parkinson's disease model.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoyu Sun,Jianyi Shen,Yimei Shu,Tianshi Wang,Lu He,Ruinan Shen,Yifan Zhou,Jinke Cheng,Suzhen Lin,Jianqing Ding

British journal of pharmacology 182:3476-3502 PubMed40164963

2025

Rhynchophylline as an agonist of sirtuin 3 ameliorates endothelial dysfunction via antagonizing mitochondrial damage of endothelial progenitor cells.

Applications

Unspecified application

Species

Unspecified reactive species

Lin Lin,Bowen Sun,Yuanlong Hu,Wenqing Yang,Jie Li,Danyang Wang,Lei Zhang,Mengkai Lu,Yuan Li,Yunlun Li,Dan Zhang,Chao Li

British journal of pharmacology 182:3017-3035 PubMed40151030

2025

Ginsenoside Rh1 mitigates mitochondrial dysfunction induced by myocardial ischaemia through its novel role as a sirtuin 3 activator.

Applications

Unspecified application

Species

Unspecified reactive species

Shuaishuai Gong,Hong Chen,Shuhua Fang,Mengyu Li,Jingui Hu,Yue Li,Boyang Yu,Junping Kou,Fang Li

Biomolecules 14: PubMed38786005

2024

Polydatin and Nicotinamide Rescue the Cellular Phenotype of Mitochondrial Diseases by Mitochondrial Unfolded Protein Response (mtUPR) Activation.

Applications

Unspecified application

Species

Unspecified reactive species

Paula Cilleros-Holgado,David Gómez-Fernández,Rocío Piñero-Pérez,José Manuel Romero Domínguez,Marta Talaverón-Rey,Diana Reche-López,Juan Miguel Suárez-Rivero,Mónica Álvarez-Córdoba,Ana Romero-González,Alejandra López-Cabrera,Marta Castro De Oliveira,Andrés Rodríguez-Sacristan,José Antonio Sánchez-Alcázar

iScience 27:109384 PubMed38550981

2024

NSAID targets SIRT3 to trigger mitochondrial dysfunction and gastric cancer cell death.

Applications

Unspecified application

Species

Unspecified reactive species

Subhashis Debsharma,Saikat Pramanik,Samik Bindu,Somnath Mazumder,Troyee Das,Uttam Pal,Debanjan Saha,Rudranil De,Shiladitya Nag,Chinmoy Banerjee,Nakul Chandra Maiti,Zhumur Ghosh,Uday Bandyopadhyay

Daru : journal of Faculty of Pharmacy, Tehran University of Medical Sciences 32:189-196 PubMed38407745

2024

Dexmedetomidine alleviates Hypoxia/reoxygenation-induced mitochondrial dysfunction in cardiomyocytes via activation of Sirt3/Prdx3 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Qingyun Tan,Wenming Dong,Qingdong Wang,Li Gao

Redox biology 69:103014 PubMed38171255

2024

Loss of cardiac mitochondrial complex I persulfidation impairs NAD homeostasis in aging.

Applications

Unspecified application

Species

Unspecified reactive species

Maria-Kyriaki Drekolia,Christina Karantanou,Ilka Wittig,Yuanyuan Li,Dominik C Fuhrmann,Bernhard Brüne,Antonia Katsouda,Jiong Hu,Andreas Papapetropoulos,Sofia-Iris Bibli

Cell research 34:13-30 PubMed38163844

2024

Hypoxia induces mitochondrial protein lactylation to limit oxidative phosphorylation.

Applications

Unspecified application

Species

Unspecified reactive species

Yunzi Mao,Jiaojiao Zhang,Qian Zhou,Xiadi He,Zhifang Zheng,Yun Wei,Kaiqiang Zhou,Yan Lin,Haowen Yu,Haihui Zhang,Yineng Zhou,Pengcheng Lin,Baixing Wu,Yiyuan Yuan,Jianyuan Zhao,Wei Xu,Shimin Zhao

Cellular and molecular life sciences : CMLS 81:12 PubMed38129330

2023

Mitochondrial impairment, decreased sirtuin activity and protein acetylation in dorsal root ganglia in Friedreich Ataxia models.

Applications

Unspecified application

Species

Unspecified reactive species

Arabela Sanz-Alcázar,Elena Britti,Fabien Delaspre,Marta Medina-Carbonero,Maria Pazos-Gil,Jordi Tamarit,Joaquim Ros,Elisa Cabiscol

EBioMedicine 98:104863 PubMed37950995

2023

Exogenous NADPH exerts a positive inotropic effect and enhances energy metabolism via SIRT3 in pathological cardiac hypertrophy and heart failure.

Applications

Unspecified application

Species

Unspecified reactive species

Ke Qian,Jie Tang,Yue-Juan Ling,Ming Zhou,Xin-Xin Yan,Yu Xie,Lu-Jia Zhu,Koju Nirmala,Kang-Yun Sun,Zheng-Hong Qin,Rui Sheng
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