Sodium Assay Kit (Colorimetric)
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(11 Publications)
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Supplier Data
Functional Studies - Sodium Assay Kit (Colorimetric) (AB211096)
Typical Sodium standard calibration curve.
- FuncS
Supplier Data
Functional Studies - Sodium Assay Kit (Colorimetric) (AB211096)
Assay Specificity : Sodium, and other mono, di and trivalent cations (15 nmol/0.15 mM each) were tested to evaluate possible interferences. Interferences were found to be < 10% when data was normalized using Sodium as 100% activity. Blank : Assay buffer/DTT.
- FuncS
Supplier Data
Functional Studies - Sodium Assay Kit (Colorimetric) (AB211096)
Estimation of sodium in human pooled serum off-the-clot (5 μL; 1 : 50 dilution) and human urine (10 μL; 1 : 100 dilution).
Product details
Sodium Assay Kit (Colorimetric) (ab211096) provides a convenient two-step method to detect sodium ions (Na+) present in serum, urine and saliva. In this assay, sodium ions present in the sample are used by the enzyme Beta-galactosidase to produce an intermediate product, which reacts with a developer to generate a color signal that can be detect at OD = 405 nm.
This kit can detect as low as 25 uM sodium in biological fluids. Endogenous mono-, di-, and trivalent ions, ascorbic acid, creatinine, glucose, urea, and bilirubin do not interfere with the assay.
Other Notes
This product was previously called K391 Sodium Assay Kit (Colorimetric). Biovision was acquired by Abcam in 2021.
Sodium (Na+) is one of the most important electrolytes along with chloride, calcium and potassium. Sodium plays vital roles in the maintenance normal cell functions such as plasma volume, pH balance or transmission of nerve impulses. Healthy individuals can absorb sodium ingested in food, and kidneys maintain proper sodium balance by excreting its excess in urine. Normal Sodium intake has been defined to be between 200-500 mg/day. Hyponatremia (low sodium concentration in blood) can occur in patients with nephrotic syndrome, excessive vomiting and diarrhea, while Hypernatremia (high sodium concentration in blood) is developed in patients suffering from liver diseases, burns, and pregnancy. Traditionally, sodium concentration in clinical settings is determined by potentiometric, gravimetry, photometry, titrimetry and flame atomic emission spectroscopy, but these methods require expensive and complex protocols that need to be performed by trained personnel.
The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.
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Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Sodium ions are essential for numerous cellular processes including membrane depolarization and action potential propagation. Sodium often works in coordination with potassium forming the sodium-potassium pump (Na+/K+ ATPase) which is part of a larger protein complex. This pump actively transports sodium out of cells while bringing potassium in maintaining gradients critical for cellular function. Sodium is involved in osmoregulation impacting the fluid balance and volume in cells and tissues.
Pathways
Sodium participates significantly in neuronal signaling and muscular contraction pathways. In neurons sodium is an important player in the action potential pathway working with voltage-gated sodium channels to propagate nerve impulses. In muscle tissue sodium is involved in the excitation-contraction coupling pathway. Sodium's function in these pathways interlinks it to proteins such as voltage-gated sodium channels in neurons and troponin in muscle cells impacting muscular contraction.
Product protocols
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Publications (11)
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Nature communications 16:5110 PubMed40467586
2025
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Nutrients 15: PubMed36678138
2023
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Nutrients 13: PubMed34444969
2021
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Diabetologia 64:181-194 PubMed33052459
2020
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Mycobiology 48:399-409 PubMed33177919
2020
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Molecular medicine reports 20:4781-4790 PubMed31638263
2019
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American journal of physiology. Heart and circulatory physiology 317:H517-H530 PubMed31172810
2019
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Acta biomaterialia 91:209-219 PubMed31029828
2019
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Biochimica et biophysica acta. Molecular basis of 1865:1284-1297 PubMed30726718
2019
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American journal of translational research 10:3430-3442 PubMed30662597
2018
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