Sodium Assay Kit (Colorimetric) (ab211096) provides a convenient two-step method to detect sodium ions (Na+) present in serum, urine and saliva.
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- Nutrients 15:2023D-Limonene Promotes Anti-Obesity in 3T3-L1 Adipocytes and High-Calorie Diet-Induced Obese Rats by Activating the AMPK Signaling Pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJin-Ting Liao,Yu-Wen Huang,Chih-Yao Hou,Jyh-Jye Wang,Chih-Chung Wu,Shu-Ling HsiehPubMed 36678138
- Nutrients 13:2021The Anti-Obesity Effects of Lemon Fermented Products in 3T3-L1 Preadipocytes and in a Rat Model with High-Calorie Diet-Induced Obesity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChih-Chung Wu,Yu-Wen Huang,Chih-Yao Hou,Ya-Ting Chen,Cheng-Di Dong,Chiu-Wen Chen,Reeta-Rani Singhania,Jie-Yin Leang,Shu-Ling HsiehPubMed 34444969
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- Molecular medicine reports 20:4781-47902019miR‑455 targets FABP4 to protect human endometrial stromal cells from cytotoxicity induced by hydrogen peroxide.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWenbo Tang et. al.PubMed 31638263
- American journal of physiology. Heart and circulatory physiology 317:H517-H5302019Renal denervation and CD161a immune ablation prevent cholinergic hypertension and renal sodium retention.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNandita Raikwar,Cameron Braverman,Peter M Snyder,Robert A Fenton,David K Meyerholz,Francois M Abboud,Sailesh C HarwaniPubMed 31172810
- Acta biomaterialia 91:209-2192019Improving hard palate wound healing using immune modulatory autotherapies.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSamir A Ballestas et. al.PubMed 31029828
- Biochimica et biophysica acta. Molecular basis of 1865:1284-12972019Chronic kidney disease induced by an adenine rich diet upregulates integrin linked kinase (ILK) and its depletion prevents the disease progression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSergio de Frutos et. al.PubMed 30726718
- American journal of translational research 10:3430-34422018Iron deficiency accelerates intervertebral disc degeneration through affecting the stability of DNA polymerase epsilon complex.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChunqiang Zhang et. al.PubMed 30662597
- International journal of molecular medicine 42:2175-21842018Role of lincRNA‑p21 in the protective effect of macrophage inhibition factor against hypoxia/serum deprivation‑induced apoptosis in mesenchymal stem cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWenzheng Xia et. al.PubMed 30015822
Key facts
- Detection method
- Colorimetric
- Sample types
- Saliva, Urine, Serum
- Assay type
- Quantitative
- Reactive species
- Mammals
- Sensitivity
- = 25 µM
Target data
What's included?
Recommended products
Sodium Assay Kit (Colorimetric) (ab211096) provides a convenient two-step method to detect sodium ions (Na+) present in serum, urine and saliva.
Key facts
- Detection method
- Colorimetric
- Sample types
- Saliva, Urine, Serum
- Assay type
- Quantitative
- Reactive species
- Mammals
- Assay Platform
- Microplate reader
- Sensitivity
- = 25 µM
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
Sodium Assay Kit (Colorimetric) (ab211096) provides a convenient two-step method to detect sodium ions (Na+) present in serum, urine and saliva. In this assay, sodium ions present in the sample are used by the enzyme β-galactosidase to produce an intermediate product, which reacts with a developer to generate a color signal that can be detect at OD = 405 nm.
This kit can detect as low as 25 μM sodium in biological fluids. Endogenous mono-, di-, and trivalent ions, ascorbic acid, creatinine, glucose, urea, and bilirubin do not interfere with the assay.
This product is manufactured by BioVision, an Abcam company and was previously called K391 Sodium Assay Kit (Colorimetric). K391-100 is the same size as the 100 test size of ab211096.
Sodium (Na+) is one of the most important electrolytes along with chloride, calcium and potassium. Sodium plays vital roles in the maintenance normal cell functions such as plasma volume, pH balance or transmission of nerve impulses. Healthy individuals can absorb sodium ingested in food, and kidneys maintain proper sodium balance by excreting its excess in urine. Normal Sodium intake has been defined to be between 200-500 mg/day. Hyponatremia (low sodium concentration in blood) can occur in patients with nephrotic syndrome, excessive vomiting and diarrhea, while Hypernatremia (high sodium concentration in blood) is developed in patients suffering from liver diseases, burns, and pregnancy. Traditionally, sodium concentration in clinical settings is determined by potentiometric, gravimetry, photometry, titrimetry and flame atomic emission spectroscopy, but these methods require expensive and complex protocols that need to be performed by trained personnel.
The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Sodium also known as Na+ functions mechanically as an electrolyte and plays an important role in generating and transmitting electrical signals across cellular membranes. It has an atomic mass of approximately 22.99 daltons. Sodium is expressed in various tissues particularly in excitable cells such as neurons and muscle cells. It functions through sodium channels which are integral membrane proteins that selectively allow sodium ions to pass through contributing to the establishment of membrane potential.
- Biological function summary
Sodium ions are essential for numerous cellular processes including membrane depolarization and action potential propagation. Sodium often works in coordination with potassium forming the sodium-potassium pump (Na+/K+ ATPase) which is part of a larger protein complex. This pump actively transports sodium out of cells while bringing potassium in maintaining gradients critical for cellular function. Sodium is involved in osmoregulation impacting the fluid balance and volume in cells and tissues.
- Pathways
Sodium participates significantly in neuronal signaling and muscular contraction pathways. In neurons sodium is an important player in the action potential pathway working with voltage-gated sodium channels to propagate nerve impulses. In muscle tissue sodium is involved in the excitation-contraction coupling pathway. Sodium's function in these pathways interlinks it to proteins such as voltage-gated sodium channels in neurons and troponin in muscle cells impacting muscular contraction.
- Associated diseases and disorders
Sodium dysregulation is connected to disorders such as hypertension and epilepsy. In hypertension abnormal sodium retention due to altered renal handling can raise blood pressure. Sodium impacts the renin-angiotensin system with changes affecting proteins like angiotensin II. In epilepsy dysfunctional sodium channels can cause excessive neuronal firing contributing to seizures. Proteins like Nav1.1 a type of sodium channel have been associated with these conditions.
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3 product images
Functional Studies - Sodium Assay Kit (Colorimetric) (ab211096)
Typical Sodium standard calibration curve.
Functional Studies - Sodium Assay Kit (Colorimetric) (ab211096)
Assay Specificity: Sodium, and other mono, di and trivalent cations (15 nmol/0.15 mM each) were tested to evaluate possible interferences. Interferences were found to be < 10% when data was normalized using Sodium as 100% activity. Blank: Assay buffer/DTT.
Functional Studies - Sodium Assay Kit (Colorimetric) (ab211096)
Estimation of sodium in human pooled serum off-the-clot (5 μL; 1:50 dilution) and human urine (10 μL; 1:100 dilution).
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