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AB240999

Soluble Epoxide Hydrolase Assay Kit

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Soluble Epoxide Hydrolase Assay Kit (ab240999) is a microplate based fluorometric kit for measuring Soluble Epoxide Hydrolase (sEH) activity in cells and tissues as well as purified protein.

View Alternative Names

Bifunctional epoxide hydrolase 2, EPHX2

3 Images
Functional Studies - Soluble Epoxide Hydrolase Assay Kit (AB240999)
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Supplier Data

Functional Studies - Soluble Epoxide Hydrolase Assay Kit (AB240999)

sEH activity in rat liver tissue lysate in absence and presence of inhibitor

Functional Studies - Soluble Epoxide Hydrolase Assay Kit (AB240999)
  • FuncS

Supplier Data

Functional Studies - Soluble Epoxide Hydrolase Assay Kit (AB240999)

Fluorescence standard curve for sEH metabolite

Functional Studies - Soluble Epoxide Hydrolase Assay Kit (AB240999)
  • FuncS

Supplier Data

Functional Studies - Soluble Epoxide Hydrolase Assay Kit (AB240999)

Enzyme kinetics in presence and absence of inhibitor for sEH positive control, and rat liver lysate (3.6 µg protein per well)

Key facts

Detection method

Fluorescent

Sample types

Purified protein, Tissue Lysate, Cell Lysate

Assay Platform

Microplate reader

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Enzyme activity assay": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Soluble Epoxide Hydrolase Assay Kit (ab240999) is a microplate based fluorometric kit for measuring Soluble Epoxide Hydrolase (sEH) activity in cells and tissues as well as purified protein. It is based on the ability of sEH to hydrolyze a non-fluorescent substrate to a fluorescent product. The kit includes a specific inhibitor for soluble epoxide hydrolase, since the substrate can be hydrolyzed by non-specific hydrolases that are present in cell and tissue lysates. Specific sEH activity can be obtained by subtracting the activity in presence of sEH inhibitor form the total activity.
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What's included?

{ "values": { "100Test": { "sellingSize": "100 Test", "publicAssetCode":"ab240999-100Test", "assetComponentDetails": [ { "size":"1 x 200 µL", "name":"sEH Substrate", "number":"AB240999-CMP05", "productcode":"" }, { "size":"1 x 1 Vial", "name":"sEH Positive Control", "number":"AB240999-CMP04", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Fluorescence Standard III", "number":"AB240999-CMP01", "productcode":"" }, { "size":"1 x 100 µL", "name":"sEH Inhibitor", "number":"AB240999-CMP03", "productcode":"" }, { "size":"1 x 25 mL", "name":"sEH Assay Buffer", "number":"AB240999-CMP02", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Epoxide hydrolase also known as EH or EPHX is an enzyme that catalyzes the conversion of epoxides to dihydrodiols. This reaction is important for detoxifying harmful compounds making epoxide hydrolase an important player in maintaining cellular health. The enzyme typically has a molecular mass around 53 kDa. Epoxide hydrolase is expressed in various tissues with significant activity observed in the liver where it contributes to the metabolism of xenobiotics. Alternate forms such as epoxide hydrolase 2 also exist each with specific functions and expression patterns.
Biological function summary

The enzyme aids in the metabolic processing of xenobiotic and endogenous epoxides reducing their potential toxicity. Epoxide hydrolase operates by hydrolyzing epoxide substrates which can be harmful if accumulated. The enzyme is not part of a known permanent complex but interacts transiently with various substrates. Its biological role extends to maintaining the equilibrium between pro-inflammatory and anti-inflammatory mediators due to its influence on lipid epoxide metabolism.

Pathways

Epoxide hydrolase plays a role in the arachidonic acid metabolic pathway where it converts epoxide intermediates into more polar diols. This action regulates the balance of eicosanoids lipid signaling molecules vital for inflammation and immune responses. The enzyme also functions alongside other detoxification enzymes such as cytochrome P450s to facilitate the biotransformation of a wide array of compounds including pharmaceutical agents.

Epoxide hydrolase is linked to several conditions. Polymorphisms in the gene encoding epoxide hydrolase have associations with susceptibility to various cancers due to altered detoxification capacities. Abnormalities in enzyme activity are also associated with cardiovascular diseases as epoxide hydrolase regulates lipid mediators that modulate blood pressure and vascular tone. In these contexts the enzyme’s regulatory role intersects with proteins involved in inflammation and vascular biology like cytochrome P450s and vascular endothelial growth factor (VEGF) influencing disease progression and treatment outcomes.
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Product protocols

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Target data

Bifunctional enzyme (PubMed : 12574510). The C-terminal domain has epoxide hydrolase activity and acts on epoxides (alkene oxides, oxiranes) and arene oxides (PubMed : 12574510, PubMed : 12869654, PubMed : 22798687). Plays a role in xenobiotic metabolism by degrading potentially toxic epoxides (By similarity). Also determines steady-state levels of physiological mediators (PubMed : 12574510, PubMed : 12869654, PubMed : 21217101, PubMed : 22798687).. Bifunctional enzyme (PubMed : 12574510). The N-terminal domain has lipid phosphatase activity, with the highest activity towards threo-9,10-phosphonooxy-hydroxy-octadecanoic acid, followed by erythro-9,10-phosphonooxy-hydroxy-octadecanoic acid, 12-phosphonooxy-octadec-9Z-enoic acid and 12-phosphonooxy-octadec-9E-enoic acid (PubMed : 12574510). Has phosphatase activity toward lyso-glycerophospholipids with also some lower activity toward lysolipids of sphingolipid and isoprenoid phosphates (PubMed : 22217705, PubMed : 22387545).
See full target information EPHX2

Additional targets

EPHX1
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Product promise

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