JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB284662

Spexyte™ Intracellular pH Calibration Buffer Kit

Be the first to review this product! Submit a review

|

(0 Publication)

Spexyte™ Intracellular pH Calibration Buffer Kit (ab284662) provides a range of pH calibration buffers (pH 4.5~ 8.0) with nigericin, which modulate the intracellular pH with the external pH in the presence of 100-150mM K+.
1 Images
Functional Studies - Spexyte™ Intracellular pH Calibration Buffer Kit (AB284662)
  • FuncS

Supplier Data

Functional Studies - Spexyte™ Intracellular pH Calibration Buffer Kit (AB284662)

Standard curve created using BCFL, AM with Spexyte™ Intracellular pH Calibration Buffer Kit (ab284662). HeLa cells were incubated with 5μM BCFL, AM for 30 minutes at room temperature. The Intracellular pH Calibration Buffer Kit was used to clamp the intracellular pH with extracellular buffers at pH 4.5 to 8.0. Intracellular pH vs. relative fluorescence ratio of Ex/Em= 440/ 530 nm and 500 nm/530 nm were plotted and a 4-parameter trendline was fitted to get the pH standard curve.

Key facts

Detection method

Fluorescent

Results type

Quantitative

style
left-column

Complementary Products

Assay Kits

AB228552

Intracellular pH Assay Kit

Functional Studies - Intracellular pH Assay Kit (AB228552)

  • 0
  • 0
Biochemicals

AB143463

BCECF-AM, intracellular ratiometric pH indicator

Chemical Structure - BCECF-AM, intracellular ratiometric pH indicator (AB143463)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Assay Kits

AB65620

Glycogen Assay Kit

Functional Studies - Glycogen Assay Kit (AB65620)

  • 0
  • 0
style
left-column

Product details

Spexyte™ Intracellular pH Calibration Buffer Kit (ab284662) provides a range of pH calibration buffers (pH 4.5~ 8.0) with nigericin, which modulate the intracellular pH with the external pH in the presence of 100-150mM K+. When used in conjunction with pH indicators, such as BCFL, AM or BCECF, AM, ab284662 can create a standard curve which is used to determine the intracellular pH.

Intracellular pH (pHi) plays an important modulating role in many cellular events, including cell volume regulation, cellular metabolism, calcium regulation, receptor-mediated signal transduction, ion transport, endocytosis, and other cellular processes. Intracellular pH is generally 6.8 ~7.4 in the cytosol and 4.5~6.0 in the acidic organelles. Intracellular pH changes have significant physiological effects, e.g., the pH-dependent concentration of intracellular messengers such as Ca2+ and cAMP affects cellular signaling. Several recent reports showed the dysregulated pH is emerging as a hallmark of cancer cells.

style
left-column

What's included?

{ "values": { "100Test": { "sellingSize": "100 Test", "publicAssetCode":"ab284662-100Test", "assetComponentDetails": [ { "size":"1 x 10 mL", "name":"pH=6 Buffer", "number":"AB284662-CMP06", "productcode":"" }, { "size":"1 x 10 mL", "name":"pH=7 Buffer", "number":"AB284662-CMP08", "productcode":"" }, { "size":"1 x 2 mg", "name":"Nigericin, free acid", "number":"AB284662-CMP02", "productcode":"" }, { "size":"1 x 10 mL", "name":"pH=5 Buffer", "number":"AB284662-CMP04", "productcode":"" }, { "size":"1 x 300 µL", "name":"DMSO", "number":"AB284662-CMP01", "productcode":"" }, { "size":"1 x 10 mL", "name":"pH=8 Buffer", "number":"AB284662-CMP10", "productcode":"" }, { "size":"1 x 10 mL", "name":"pH=5.5 Buffer", "number":"AB284662-CMP05", "productcode":"" }, { "size":"1 x 10 mL", "name":"pH=7.5 Buffer", "number":"AB284662-CMP09", "productcode":"" }, { "size":"1 x 10 mL", "name":"pH=6.5 Buffer", "number":"AB284662-CMP07", "productcode":"" }, { "size":"1 x 10 mL", "name":"pH=4.5 Buffer", "number":"AB284662-CMP03", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
style
left-column
style
left-column

Product protocols

style
left-column

Target data

style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com