Sphingomyelin Assay Kit

Product details

Sphingomyelin Assay Kit (ab133118) provides a specific, sensitive, and convenient method for quantifying sphingomyelin in plasma or serum. In this assay, sphingomyelinase is first used to hydrolyze sphingomyelin to phosphorylcholine and ceramide. Alkaline phosphatase then generates choline from the phosphorylcholine and the newly formed choline is used to generate hydrogen peroxide in a reaction catalyzed by choline oxidase. Finally, with peroxidase as a catalyst, hydrogen peroxide reacts with DAOS and 4-aminoantipyrine to generate a blue color with an optimal absorption at OD595 nm.

Under the standardized conditions of the assay described in this booklet, the dynamic range of the kit is 5-50 mg/dL sphingomyelin.

Other Notes
Sphingomyelin (ceramide phosphorylcholine) is an important lipid component of cell membranes and lipoproteins. It consists of a ceramide moiety linked via a phosphodiester bond to phosphorylcholine. Sphingomyelinases are a family of enzymes that can hydrolyze sphingomyelin into ceramide and phosphorylcholine. Ceramides have been implicated as key mediators in signaling pathways, with outcomes as diverse as cell proliferation, differentiation, growth arrest, and apoptosis. An inherited deficiency of acid sphingomyelinase activity results in the sphingomyelin storage disorder Niemann- Pick disease. This disease results in the accumulation of sphinogmyelin in cells, tissues, and fluids. Since sphingomyelin has been implicated in the pathogenesis of several diseases, including atherosclerosis, sensitive and reliable techniques for its quantification are of considerable importance.

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