Spinal Astrocytes - Human iPSC derived cells (Male, WC-30)

Product details

These products are extremely sensitive and require specific conditions to maintain cell health and functionality. Please refer to the recommended protocols for the correct methodology in plating and maintaining these cells.

For even greater physiological relevance, co-culture protocols are available for use with Spinal Motor Neurons (ab315364).

Human Spinal Astrocytes are derived from normal control iPSC line WC-30. BrainXell Human Spinal Astrocytes express key astrocyte markers GFAP and S100β at >80% and exhibit mature process-bearing morphologies as demonstrated by ICC labelling. Labelling by the spinal-specific marker HOXB4 in ICC highlights the regional specificity of the Spinal Astrocytes. Spinal Astrocytes promote mature motor neuron activity. Co-culturing motor neurons with spinal astrocytes promotes maximal calcium influx within 10 days. Co-culture spinal astrocytes with motor neurons also accelerates the development of activity recorded by multi-electrode array.

Vials of Spinal Astrocytes are delivered with two supplements which are designed to facilitate the maturation and health of the cultures. BrainFast Astro is a seeding supplement which promotes cell survival at thaw and completes the differentiation process to astrocytes. BrainFast SK enhances the purity of the culture.

In partnership with BrainXell