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AB315365

Spinal Motor Neuron - Human iPSC derived cells (GFP - expressing) (Male, WC-30)

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These products are extremely sensitive and require specific conditions to maintain cell health and functionality. Please refer to the recommended protocols for the correct methodology in plating and maintaining these cells.
3 Images
Immunocytochemistry/ Immunofluorescence - Spinal Motor Neuron - Human iPSC derived cells (GFP - expressing) (Male, WC-30) (AB315365)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Spinal Motor Neuron - Human iPSC derived cells (GFP - expressing) (Male, WC-30) (AB315365)

This image was produced using ab315364 - the non GFP-expressing cells.

Marker Expression : Spinal Motor Neurons (ab315364) have high neuronal purity (>90%) and consist of greater than 70% motor neurons. Labeling with the pan-neuronal marker MAP2 (green) and the motor neuron-specific marker FOXP1 (red) highlight the purity of this neuronal product.

Functional Studies - Spinal Motor Neuron - Human iPSC derived cells (GFP - expressing) (Male, WC-30) (AB315365)
  • FuncS

Supplier Data

Functional Studies - Spinal Motor Neuron - Human iPSC derived cells (GFP - expressing) (Male, WC-30) (AB315365)

This image was produced using ab315364 - the non GFP-expressing cells. Function : Spinal Motor Neurons (ab315364) exhibit pronounced electrophysiological activity after two weeks in culture, as demonstrated by multi-electrode array (MEA) recordings.

Immunocytochemistry/ Immunofluorescence - Spinal Motor Neuron - Human iPSC derived cells (GFP - expressing) (Male, WC-30) (AB315365)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Spinal Motor Neuron - Human iPSC derived cells (GFP - expressing) (Male, WC-30) (AB315365)

This image was produced using ab315364 - the non GFP-expressing cells. Morphology : Spinal Motor Neurons (ab315364) are adherent and exhibit substantial neurite outgrowth within the first week in culture. Calcein staining (green) demonstrates the elaborate processes of the Spinal Motor Neurons in culture.

Key facts

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ICC/IF": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

Product details

These products are extremely sensitive and require specific conditions to maintain cell health and functionality. Please refer to the recommended protocols for the correct methodology in plating and maintaining these cells.

This product is a GFP expressing alternative to (ab315364).

Human Spinal Motor Neurons are derived from normal control iPSC line WC-30, ubiquitously expressing eGFP. BrainXell Spinal Motor Neurons have high neuronal purity (>80%) consisting of greater than 70% motor neurons. Labelling with the pan-neuronal marker MAP2 and the motor neuron-specific marker FOXP1 highlight the purity of this neuronal product. Spinal Motor Neurons are adherent and exhibit substantial neurite outgrowth within the first week in culture and demonstrate pronounced electrophysiological activity after two weeks in culture, as demonstrated by multi-electrode array (MEA) recordings.

Vials of Spinal Motor Neurons are delivered with three supplements which are designed to facilitate the maturation and health of the cultures. BrainFast Motor is a seeding supplement which promotes cell survival at thaw and completes the differentiation process from late-stage neural progenitor to neuron. BrainFast SK enhances neuronal purity, and BrainFast D4 accelerates maturation.

In partnership with BrainXell

What's included?

{ "values": { "2000000Cellsvial": { "sellingSize": "2000000 Cells/vial", "publicAssetCode":"ab315365-2000000Cells_vial", "assetComponentDetails": [ { "size":"1 x 100 µL", "name":"BrainFast Motor Supplement (1000x)", "number":"AB315365-CMP02", "productcode":"" }, { "size":"1 x 100 µL", "name":"BrainFast D4 Supplement (1000x)", "number":"AB315365-CMP04", "productcode":"" }, { "size":"1 x 100 µL", "name":"BrainFast SK Supplement (1000x)", "number":"AB315365-CMP03", "productcode":"" }, { "size":"1 x 2000000 Cells/vial", "name":"Human Spinal Motor Neurons (GFP-Expressing) (Male, WC-30)", "number":"AB315365-CMP01", "productcode":"" } ] } } }

Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-196°C
Appropriate long-term storage conditions
-196°C
Storage information
Please refer to protocols

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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