SRB Assay / Sulforhodamine B Assay Kit ab235935 relies on the stoichiometric binding of SRB (Sulforhodamine B) dye to proteins under mild acidic conditions and its subsequent extraction under basic conditions.
Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
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- Natural products and bioprospecting 13:342023Marine natural product lepadin A as a novel inducer of immunogenic cell death via CD91-dependent pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDalila Carbone,Carmela Gallo,Genoveffa Nuzzo,Giusi Barra,Mario Dell'Isola,Mario Affuso,Olimpia Follero,Federica Albiani,Clementina Sansone,Emiliano Manzo,Giuliana d'Ippolito,Angelo FontanaPubMed 37779162
- Nutrients 15:2023Furanocoumarin Notopterol: Inhibition of Hepatocellular Carcinogenesis through Suppression of Cancer Stemness Signaling and Induction of Oxidative Stress-Associated Cell Death.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTing-Yun Huang,Ching-Kuo Yang,Ming-Yao Chen,Vijesh Kumar Yadav,Iat-Hang Fong,Chi-Tai Yeh,Yih-Giun CherngPubMed 37299411
- Clinical, cosmetic and investigational dermatology 16:565-5802023MicroRNA-182-5p Inhibits Hypertrophic Scar Formation by Inhibiting the Proliferation and Migration of Fibroblasts via SMAD4 Pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMingzhu Jin,Xiao XuPubMed 36919011
- The Journal of biological chemistry 299:1027682022Mapping the KRAS proteoform landscape in colorectal cancer identifies truncated KRAS4B that decreases MAPK signaling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLauren M Adams,Caroline J DeHart,Bryon S Drown,Lissa C Anderson,William Bocik,Emily S Boja,Tara M Hiltke,Christopher L Hendrickson,Henry Rodriguez,Michael Caldwell,Reza Vafabakhsh,Neil L KelleherPubMed 36470426
- Pharmaceutics 14:2022Nano-Lipids Based on Ginger Oil and Lecithin as a Potential Drug Delivery System.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHung Quach,Tuong-Vi Le,Thanh-Thuy Nguyen,Phuong Nguyen,Cuu Khoa Nguyen,Le Hang DangPubMed 36015280
- Stem cell research & therapy 13:2692022Tideglusib promotes wound healing in aged skin by activating PI3K/Akt pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJiachen Sun,Hongqing Zhao,Chuan'an Shen,Shiyi Li,Wen Zhang,Jinglong Ma,Zhisheng Li,Ming Zhang,Jianqiu YangPubMed 35729652
- Scientific reports 12:36182022Deprogramming metabolism in pancreatic cancer with a bi-functional GPR55 inhibitor and biased β adrenergic agonist.Applications:Unspecified applicationReactive species:Unspecified reactive speciesArtur Wnorowski,Danuta Dudzik,Michel Bernier,Jakub Wójcik,Guido Keijzers,Alberto Diaz-Ruiz,Karolina Mazur,Yongqing Zhang,Haiyong Han,Morten Scheibye-Knudsen,Krzysztof Jozwiak,Coral Barbas,Irving W WainerPubMed 35256673
- Nutrients 14:2022Oligosaccharides Ameliorate Acute Kidney Injury by Alleviating Cluster of Differentiation 44-Mediated Immune Responses in Renal Tubular Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTso-Hsiao Chen,Chung-Te Liu,Chung-Yi Cheng,Yuh-Mou Sue,Nai-Jen Huang,Cheng-Hsien ChenPubMed 35215410
- Biomolecules 12:2022Identification of the Marine Alkaloid Lepadin A as Potential Inducer of Immunogenic Cell Death.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGenoveffa Nuzzo,Carmela Gallo,Fabio Crocetta,Lucia Romano,Giusi Barra,Giuseppina Senese,Mario dell'Isola,Dalila Carbone,Valentina Tanduo,Federica Albiani,Guido Villani,Giuliana d'Ippolito,Emiliano Manzo,Angelo FontanaPubMed 35204747
- Marine drugs 18:2020A New Bioassay Platform Design for the Discovery of Small Molecules with Anticancer Immunotherapeutic Activity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCarmela Gallo et. al.PubMed 33260400
Key facts
- Detection method
- Colorimetric
- Sample types
- Adherent cells
- Assay type
- Cell-based (quantitative)
Target data
What's included?
Recommended products
SRB Assay / Sulforhodamine B Assay Kit ab235935 relies on the stoichiometric binding of SRB (Sulforhodamine B) dye to proteins under mild acidic conditions and its subsequent extraction under basic conditions.
Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
Key facts
- Detection method
- Colorimetric
- Sample types
- Adherent cells
- Assay type
- Cell-based (quantitative)
- Assay Platform
- Microplate reader
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
SRB Assay / Sulforhodamine B Assay Kit ab235935 is used as a cell viability assay.
SRB assay principle
The SRB Assay / Sulforhodamine B Assay relies on the stoichiometric binding of SRB (Sulforhodamine B) dye to proteins under mild acidic conditions and its subsequent extraction under basic conditions. The amount of dye extracted is a proxy for cell mass and thus the number of cells in a sample.
The measurement of the number of cells in the assay allows its use for analysis of cell viability, cytotoxicity and cell proliferation.
In the assay, cultured cells are fixed on plates, stained with Sulforhodamine B, washed and dried, then the bound dye is solubilized and the absorbance of the dye in solution is measured at OD 565 nm.
Review our cell health assay guide to learn about cell viability assays and cytotoxicity assays, and apoptosis assays.
This product is manufactured by BioVision, an Abcam company and was previously called K943 Sulforhodamine B Cell Cytotoxicity Assay Kit (Colorimetric). K943-1000 is the same size as the 1000 test size of ab235935.
The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Sulforhodamine B often referred to as SRB is a dye commonly used in biological assays. It has a mass of approximately 580.7 g/mol. Researchers extensively use SRB in cell proliferation and cytotoxicity assays due to its ability to bind to protein components. It is not naturally expressed in biological organisms since it is a synthetic compound. Instead scientists introduce it into cell cultures and tissues for experimental purposes to measure cellular protein content.
- Biological function summary
The compound plays an essential role as a cell stain that helps in visualizing and quantifying cell growth. It binds to basic amino acid residues of proteins in cells allowing consistent measurement of cell density. SRB does not function as part of any molecular complex as its utility lies mainly in providing a colorimetric means for analyzing cell growth and viability.
- Pathways
SRB serves as a significant tool rather than a participant in cellular pathways. It helps researchers assess cell metabolic pathways by measuring changes in protein synthesis. Although not part of the pathways it indirectly interacts with pathways such as apoptosis and cell cycle regulation through its usage in assays. SRB when bound to proteins aids in the evaluation of complex interactions within the cell helping scientists to infer the activity of other proteins involved in these pathways.
- Associated diseases and disorders
SRB aids in the research of cancer and other proliferative diseases. It is instrumental in drug screening assays to evaluate the effects of anti-cancer drugs on cell growth and survival. Although not directly linked to diseases its role in determining cell viability post-treatment assists researchers in understanding how drugs interact with proteins like p53 and caspases which are important in cancer pathogenesis and cell death processes.
Product promise
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Full details and terms and conditions can be found here:
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Functional Studies - SRB Assay / Sulforhodamine B Assay Kit (ab235935)
Dose-response curve of HepG2 (a), MCF7 (b) and HEK-293 (c) cells, after exposure to doxorubicin for 72 hours, as determined using the Sulforhodamine B Cell Cytotoxicity Assay Kit (Colorimetric). Assays were performed according to the kit protocol in triplicate.
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