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AB235935

SRB Assay / Sulforhodamine B Assay Kit

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(15 Publications)

SRB Assay / Sulforhodamine B Assay Kit ab235935 relies on the stoichiometric binding of SRB (Sulforhodamine B) dye to proteins under mild acidic conditions and its subsequent extraction under basic conditions.

Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
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Functional Studies - SRB Assay / Sulforhodamine B Assay Kit (AB235935)
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Supplier Data

Functional Studies - SRB Assay / Sulforhodamine B Assay Kit (AB235935)

Dose-response curve of HepG2 (a), MCF7 (b) and HEK-293 (c) cells, after exposure to doxorubicin for 72 hours, as determined using the Sulforhodamine B Cell Cytotoxicity Assay Kit (Colorimetric). Assays were performed according to the kit protocol in triplicate.

Key facts

Detection method

Colorimetric

Sample types

Adherent cells

Assay type

Cell-based

Results type

Quantitative

Assay Platform

Microplate reader

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Product details

SRB Assay / Sulforhodamine B Assay Kit ab235935 is used as a cell viability assay.

SRB assay principle

The SRB Assay / Sulforhodamine B Assay relies on the stoichiometric binding of SRB (Sulforhodamine B) dye to proteins under mild acidic conditions and its subsequent extraction under basic conditions. The amount of dye extracted is a proxy for cell mass and thus the number of cells in a sample.

The measurement of the number of cells in the assay allows its use for analysis of cell viability, cytotoxicity and cell proliferation.

In the assay, cultured cells are fixed on plates, stained with Sulforhodamine B, washed and dried, then the bound dye is solubilized and the absorbance of the dye in solution is measured at OD 565 nm.

Review our cell health assay guide to learn about cell viability assays and cytotoxicity assays, and apoptosis assays.

This product is manufactured by BioVision, an Abcam company and was previously called K943 Sulforhodamine B Cell Cytotoxicity Assay Kit (Colorimetric). K943-1000 is the same size as the 1000 test size of ab235935.

The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.

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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Sulforhodamine B often referred to as SRB is a dye commonly used in biological assays. It has a mass of approximately 580.7 g/mol. Researchers extensively use SRB in cell proliferation and cytotoxicity assays due to its ability to bind to protein components. It is not naturally expressed in biological organisms since it is a synthetic compound. Instead scientists introduce it into cell cultures and tissues for experimental purposes to measure cellular protein content.
Biological function summary

The compound plays an essential role as a cell stain that helps in visualizing and quantifying cell growth. It binds to basic amino acid residues of proteins in cells allowing consistent measurement of cell density. SRB does not function as part of any molecular complex as its utility lies mainly in providing a colorimetric means for analyzing cell growth and viability.

Pathways

SRB serves as a significant tool rather than a participant in cellular pathways. It helps researchers assess cell metabolic pathways by measuring changes in protein synthesis. Although not part of the pathways it indirectly interacts with pathways such as apoptosis and cell cycle regulation through its usage in assays. SRB when bound to proteins aids in the evaluation of complex interactions within the cell helping scientists to infer the activity of other proteins involved in these pathways.

SRB aids in the research of cancer and other proliferative diseases. It is instrumental in drug screening assays to evaluate the effects of anti-cancer drugs on cell growth and survival. Although not directly linked to diseases its role in determining cell viability post-treatment assists researchers in understanding how drugs interact with proteins like p53 and caspases which are important in cancer pathogenesis and cell death processes.
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Product protocols

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Target data

See full target information Sulforhodamine B
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Publications (15)

Recent publications for all applications. Explore the full list and refine your search

Renal failure 46:2384586 PubMed39082695

2024

Pressure induces peritoneal fibrosis and inflammation through CD44 signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Yu-Wei Chen,Chia-Te Liao,Mei-Yi Wu,Nai-Jen Huang,Yih-Giun Cherng,Mai-Szu Wu,Yung-Ho Hsu,Cheng-Hsien Chen

Journal of cannabis research 6:22 PubMed38720356

2024

Cannabidiol's cytotoxicity in pancreatic cancer is induced via an upregulation of ceramide synthase 1 and ER stress.

Applications

Unspecified application

Species

Unspecified reactive species

Nagina Mangal,Vikash Reebye,Nagy Habib,Mikael H Sodergren

Marine drugs 22: PubMed38393046

2024

Chemical and Pharmacological Prospection of the Ascidian .

Applications

Unspecified application

Species

Unspecified reactive species

Pedro Jatai Batista,Genoveffa Nuzzo,Carmela Gallo,Dalila Carbone,Mario dell'Isola,Mario Affuso,Giusi Barra,Federica Albiani,Fabio Crocetta,Riccardo Virgili,Valerio Mazzella,Daniela Castiglia,Giuliana d'Ippolito,Emiliano Manzo,Angelo Fontana

Natural products and bioprospecting 13:34 PubMed37779162

2023

Marine natural product lepadin A as a novel inducer of immunogenic cell death via CD91-dependent pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Dalila Carbone,Carmela Gallo,Genoveffa Nuzzo,Giusi Barra,Mario Dell'Isola,Mario Affuso,Olimpia Follero,Federica Albiani,Clementina Sansone,Emiliano Manzo,Giuliana d'Ippolito,Angelo Fontana

Nutrients 15: PubMed37299411

2023

Furanocoumarin Notopterol: Inhibition of Hepatocellular Carcinogenesis through Suppression of Cancer Stemness Signaling and Induction of Oxidative Stress-Associated Cell Death.

Applications

Unspecified application

Species

Unspecified reactive species

Ting-Yun Huang,Ching-Kuo Yang,Ming-Yao Chen,Vijesh Kumar Yadav,Iat-Hang Fong,Chi-Tai Yeh,Yih-Giun Cherng

Free radical biology & medicine 204:243-251 PubMed37179033

2023

An antioxidant response element regulates the HIF1α axis in breast cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Sarah E Lacher,Cara Skon-Hegg,Brian L Ruis,Jennifer Krznarich,Matthew Slattery

Clinical, cosmetic and investigational dermatology 16:565-580 PubMed36919011

2023

MicroRNA-182-5p Inhibits Hypertrophic Scar Formation by Inhibiting the Proliferation and Migration of Fibroblasts via SMAD4 Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Mingzhu Jin,Xiao Xu

The Journal of biological chemistry 299:102768 PubMed36470426

2022

Mapping the KRAS proteoform landscape in colorectal cancer identifies truncated KRAS4B that decreases MAPK signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Lauren M Adams,Caroline J DeHart,Bryon S Drown,Lissa C Anderson,William Bocik,Emily S Boja,Tara M Hiltke,Christopher L Hendrickson,Henry Rodriguez,Michael Caldwell,Reza Vafabakhsh,Neil L Kelleher

Pharmaceutics 14: PubMed36015280

2022

Nano-Lipids Based on Ginger Oil and Lecithin as a Potential Drug Delivery System.

Applications

Unspecified application

Species

Unspecified reactive species

Hung Quach,Tuong-Vi Le,Thanh-Thuy Nguyen,Phuong Nguyen,Cuu Khoa Nguyen,Le Hang Dang

Stem cell research & therapy 13:269 PubMed35729652

2022

Tideglusib promotes wound healing in aged skin by activating PI3K/Akt pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jiachen Sun,Hongqing Zhao,Chuan'an Shen,Shiyi Li,Wen Zhang,Jinglong Ma,Zhisheng Li,Ming Zhang,Jianqiu Yang
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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