AB133111

SREBP-2 Transcription Factor Assay Kit

Name: SREBP-2 Transcription Factor Assay Kit (ab133111) | Abcam
Brand: Abcam Limited
SKU: AB133111
Price: 750 USD
Availability: InStock

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(4 Publications)

Abcam's SREBP-2 Transcription Factor Assay Kit (ab133111) is a non-radioactive, sensitive method for detecting specific transcription factor DNA binding activity in nuclear extracts and whole cell lysates.

View Alternative Names

BHLHD2, SREBP2, SREBF2, Sterol regulatory element-binding protein 2, SREBP-2, Class D basic helix-loop-helix protein 2, Sterol regulatory element-binding transcription factor 2, bHLHd2

3 Images

Functional Studies - SREBP-2 Transcription Factor Assay Kit (AB133111)

FuncS

Lab

Functional Studies - SREBP-2 Transcription Factor Assay Kit (AB133111)

Positive control (10 or 5 uL) with or without inhibitor (10 or 2 uL) (duplicates; +/- SD).

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Supplier Data

Functional Studies - SREBP-2 Transcription Factor Assay Kit (AB133111)

Example of typical standard curve obtained using ab133111.

FuncS

Lab

Functional Studies - SREBP-2 Transcription Factor Assay Kit (AB133111)

HepG2 cells were grown in protein free hybridoma medium overnight (Life Technologies), followed by a 30 minutes incubation with the addition of 1% (2-Hydroxypropyl)-β-cyclodextrin (Sigma) or with the supplement of liqued cholesterol (Life Technologies). 10 uL of nuclear extracts (ab113474) were tested, corresponding to 2.5e5 cells per well.

Key facts

Detection method

Colorimetric

Sample types

Nuclear Extracts, Suspension cells, Adherent cells

Reacts with

Human

Assay type

Semi-quantitative

Assay Platform

Microplate reader

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Product details

A 96-well enzyme-linked immunosorbent assay (ELISA) replaces the cumbersome radioactive electrophoretic mobility shift assay (EMSA). A specific double stranded DNA (dsDNA) sequence containing the SREBP response element is immobilized to the wells of a 96-well plate. SREBP contained in a nuclear extract, binds specifically to the SREBP response element. SREBP is detected by addition of a specific primary antibody directed against SREBP. A secondary antibody conjugated to HRP is added to provide a sensitive colorimetric readout at 450 nm.

Lipid homeostasis in vertebrate cells is regulated by a family of transcription factors called sterol regulatory elements binding proteins (SREBPs). SREBPs directly activate the expression of over 30 gened involved in teh synthesis and uptake of cholesterol, fatty cids, triglycerides and phopholipids. SREBP-2 activates cholesterol synthesis by upregulating expression of HMG-CoA reductase.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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What's included?

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Properties and storage information

Shipped at conditions

Dry Ice

Appropriate short-term storage conditions

Multi

Appropriate long-term storage conditions

Multi

Storage information

Please refer to protocols

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Product protocols

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Target data

Sterol regulatory element-binding protein 2. Precursor of the transcription factor form (Processed sterol regulatory element-binding protein 2), which is embedded in the endoplasmic reticulum membrane (PubMed : 32322062). Low sterol concentrations promote processing of this form, releasing the transcription factor form that translocates into the nucleus and activates transcription of genes involved in cholesterol biosynthesis (PubMed : 32322062).. Processed sterol regulatory element-binding protein 2. Key transcription factor that regulates expression of genes involved in cholesterol biosynthesis (PubMed : 12177166, PubMed : 32322062). Binds to the sterol regulatory element 1 (SRE-1) (5'-ATCACCCCAC-3'). Has dual sequence specificity binding to both an E-box motif (5'-ATCACGTGA-3') and to SRE-1 (5'-ATCACCCCAC-3') (PubMed : 12177166, PubMed : 7903453). Regulates transcription of genes related to cholesterol synthesis pathway (PubMed : 12177166, PubMed : 32322062).

See full target information SREBF2

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in microbiology 13:849020 PubMed35495674

2022

LncRNA NEAT1 Potentiates SREBP2 Activity to Promote Inflammatory Macrophage Activation and Limit Hantaan Virus Propagation.

Applications

Unspecified application

Species

Unspecified reactive species

Yongheng Yang,Mengyun Li,Yongtao Ma,Wei Ye,Yue Si,Xuyang Zheng,He Liu,Linfeng Cheng,Liang Zhang,Hui Zhang,Xijing Zhang,Yingfeng Lei,Lixin Shen,Fanglin Zhang,Hongwei Ma

Nano today 38:101149 PubMed33846686

2021

Lung-selective 25-hydroxycholesterol nanotherapeutics as a suppressor of COVID-19-associated cytokine storm.

Applications

Unspecified application

Species

Unspecified reactive species

Hyelim Kim,Han Sol Lee,June Hong Ahn,Kyung Soo Hong,Jong Geol Jang,Jiseon An,Yong-Hyeon Mun,So-Yeol Yoo,Yoon Jung Choi,Mi-Young Yun,Gyu Yong Song,Jinmyoung Joo,Dong Hee Na,Hong Nam Kim,Hee Ho Park,Jae-Young Lee,Wonhwa Lee

Signal transduction and targeted therapy 5:186 PubMed32883951

2020

COVID-19-activated SREBP2 disturbs cholesterol biosynthesis and leads to cytokine storm.

Applications

Unspecified application

Species

Unspecified reactive species

Wonhwa Lee,June Hong Ahn,Hee Ho Park,Hong Nam Kim,Hyelim Kim,Youngbum Yoo,Hyosoo Shin,Kyung Soo Hong,Jong Geol Jang,Chun Gwon Park,Eun Young Choi,Jong-Sup Bae,Young-Kyo Seo

Molecular neurobiology 56:5256-5269 PubMed30569418

2018

Palmitate-Induced SREBP1 Expression and Activation Underlies the Increased BACE 1 Activity and Amyloid Beta Genesis.

Applications

Unspecified application

Species

Unspecified reactive species

Gurdeep Marwarha,Kate Claycombe-Larson,Jonah Lund,Othman Ghribi

View all publications

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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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SREBP-2 Transcription Factor Assay Kit

Functional Studies - SREBP-2 Transcription Factor Assay Kit (AB133111)

Functional Studies - SREBP-2 Transcription Factor Assay Kit (AB133111)

Functional Studies - SREBP-2 Transcription Factor Assay Kit (AB133111)

Key facts

Detection method

Sample types

Reacts with

Assay type

Assay Platform

Product details

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (4)

Complementary Products

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Secondary Antibodies

Sample Prep & Detection Kits

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