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Succinate Assay Kit (Colorimetric) ab204718 is a sensitive, fast and easy-to-use kit.

Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.

Is this your first time purchasing this assay kit? Get 20% off one kit by entering code 20OFF-T5A8Q at checkout. T&C’s apply – click here. Offer only valid when purchasing directly from Abcam in Europe, North America, Australia, and Singapore.


Images

Key facts

Detection method

Colorimetric

Sample types

Tissue, Suspension cells, Adherent cells

Assay type

Quantitative

Reactive species

Mammals

Assay time

30m

Sensitivity

= 40 µM

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Alternative names

What's included?

100 Test
Components
Assay Buffer VII
1 x 25 mL
Developer Solution III
1 x 1 Vial
Enzyme Mix XI
1 x 1 Vial
Succinate Converter
1 x 1 Vial
Succinate Standard
1 x 1 Vial
Succinate Substrate Mix
1 x 1 Vial

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Succinate Assay Kit (Colorimetric) ab204718 is a sensitive, fast and easy-to-use kit.

Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.

Is this your first time purchasing this assay kit? Get 20% off one kit by entering code 20OFF-T5A8Q at checkout. T&C’s apply – click here. Offer only valid when purchasing directly from Abcam in Europe, North America, Australia, and Singapore.

Alternative names

Key facts

Detection method

Colorimetric

Sample types

Tissue, Suspension cells, Adherent cells

Assay type

Quantitative

Reactive species

Mammals

Assay time

30m

Assay Platform

Microplate reader

Sensitivity

= 40 µM

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

-20°C

Notes

Succinate Assay Kit (Colorimetric) (ab204718) is a sensitive, fast and easy-to-use kit. In the succinate assay protocol, Succinate is utilized by Succinyl-CoA Synthetase to form an intermediate, which undergoes a series of reactions and reduces a colorless probe to a colored product with strong absorbance at OD=450 nm. This assay kit can detect less than 40 μM of succinate or succinic acid in a variety of sample types.

Succinate + ATP + CoA converted by succiny-CoA synthase to succiny-CoA + ADP + Pi with Color Detection (OD 450 nm).

Succinate assay protocol summary:
- add samples and standards to wells
- add reaction mix and incubate for 30 min
- analyze with a microplate reader

This product is manufactured by BioVision, an Abcam company and was previously called K649 Succinate (Succinic Acid) Colorimetric Assay Kit. K649-100 is the same size as the 100 test size of ab204718.

Succinic acid (C4H6O4) is distributed in all plants and animal tissues and was first obtained from amber. Due to its low toxicity, it is widely used in agriculture, food and pharmaceutical industry. Succinate (C4H4O4) is the salt or ester of succinic acid. It is one of the most active components of cellular respiratory and intracellular energy generation. Measurement of succinate or succinic acid level is a key to analysis of the citric acid cycle.

This kit has so far only been tested with human and rat samples. Since Succinate is distributed in all plants and animal tissues this kit should detect Succinate independent of the species. Depending on the sample type, the sample preparation might need to be optimized.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Functional Studies - Succinate Assay Kit (Colorimetric) (ab204718), expandable thumbnail

    Functional Studies - Succinate Assay Kit (Colorimetric) (ab204718)

    Succinate Standard Curve.

  • Functional Studies - Succinate Assay Kit (Colorimetric) (ab204718), expandable thumbnail

    Functional Studies - Succinate Assay Kit (Colorimetric) (ab204718)

    Measurement of Succinate levels in rat liver (40 μg), kidney (40 μg) and muscle (40 μg), Jurkat cell lysate (200 μg) and white wine (4 μl, wine sample was diluted 1:1 with 0.5 M Tris HCl, pH 8.0).

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

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