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Succinate Assay Kit (Colorimetric) (ab204718) provides a simple and fast method to quantify succinate levels. Readout on any colorimetric (450 nm) plate reader.

- Cited in over 40 publications
- Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.


Images

Functional Studies - Succinate Assay Kit (Colorimetric) (AB204718), expandable thumbnail
  • Functional Studies - Succinate Assay Kit (Colorimetric) (AB204718), expandable thumbnail

Publications

Key facts

Detection method
Colorimetric
Sample types
Tissue, Suspension cells, Adherent cells
Assay type
Quantitative
Reactive species
Mammals
Assay time
30m
Sensitivity
= 40 µM

Alternative names

What's included?

100 Test
Components
Assay Buffer 7
1 x 25 mL
Developer Solution III
1 x 1 Vial
Enzyme Mix XI
1 x 1 Vial
Succinate Converter
1 x 1 Vial
Succinate Standard
1 x 1 Vial
Succinate Substrate Mix
1 x 1 Vial

Recommended products

Succinate Assay Kit (Colorimetric) (ab204718) provides a simple and fast method to quantify succinate levels. Readout on any colorimetric (450 nm) plate reader.

- Cited in over 40 publications
- Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.

Key facts

Detection method
Colorimetric
Sample types
Tissue, Suspension cells, Adherent cells
Assay type
Quantitative
Reactive species
Mammals
Assay time
30m
Assay Platform
Microplate reader
Sensitivity
= 40 µM

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C

Notes

How the assay works

In Succinate Assay Kit protocol, Succinate is utilized by Succinyl-CoA Synthetase to form an intermediate, which undergoes a series of reactions and reduces a colorless probe to a colored product with strong absorbance at OD=450 nm.
Succinate + ATP + CoA converted by succiny-CoA synthase to succiny-CoA + ADP + Pi with Color Detection (OD 450 nm).
This kit has so far only been tested with human and rat samples. Since Succinate is distributed in all plants and animal tissues this kit should detect Succinate independent of the species. Depending on the sample type, the sample preparation might need to be optimized.

Succinate assay protocol summary:

- Add samples and standards to wells
- Add reaction mix and incubate for 30 min
- Analyze with a microplate reader

How other researchers are using

Succinate Assay Kit (Colorimetric)has been used in a variety of sample type including:
- Mouse brain 1
- IPSC-derived cardiomyocytes 2
- Human melanoma (C8161) cells 3
References: 

1-Lancaster M et al. 2023
2-Srivastava S et al. 2023
3-Shen L et al. 2023

This product is manufactured by BioVision, an Abcam company and was previously called K649 Succinate (Succinic Acid) Colorimetric Assay Kit. K649-100 is the same size as the 100 test size of ab204718.

Succinic acid (C4H6O4) is distributed in all plants and animal tissues and was first obtained from amber. Due to its low toxicity, it is widely used in agriculture, food and pharmaceutical industry. Succinate (C4H4O4) is the salt or ester of succinic acid. It is one of the most active components of cellular respiratory and intracellular energy generation. Measurement of succinate or succinic acid level is a key to analysis of the citric acid cycle.

This kit has so far only been tested with human and rat samples. Since Succinate is distributed in all plants and animal tissues this kit should detect Succinate independent of the species. Depending on the sample type, the sample preparation might need to be optimized.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Succinic acid also known as butanedioic acid plays a significant mechanical role in cellular metabolism. It has a molecular mass of 118.09 g/mol and is important for both prokaryotic and eukaryotic cells. This compound is expressed in mitochondria where it acts as an intermediary in critical metabolic processes. The widespread presence across different types of organisms highlights its essential role in cellular energy production.

Biological function summary

Succinic acid contributes to energy production by participating in the tricarboxylic acid (TCA) cycle also known as the Krebs cycle. It is part of this complex cycle that produces ATP the energy currency of the cell. Succinic acid links different metabolic pathways facilitating the oxidation of nutrients to release energy. This has a broad impact on cellular function and organismal energy homeostasis emphasizing its indispensable role in general metabolism.

Pathways

Succinic acid is heavily involved in the TCA cycle and the electron transport chain. During the TCA cycle it transforms into fumarate through succinate dehydrogenase a vital process for energy extraction in cells. Succinic acid influences the electron transport chain where it facilitates ATP synthesis by interacting with complexes like Coenzyme Q10. These pathways are central to metabolic activity demonstrating how succinic acid integrates into the broader network of cellular respiration.

Associated diseases and disorders

Succinic acid shows connections with metabolic disorders such as mitochondrial diseases and some cancers. Abnormal accumulation or defective metabolism of succinic acid can lead to mitochondrial dysfunction contributing to disorders like Leigh's syndrome. Additionally its involvement with the succinate dehydrogenase complex indicates potential links to various cancers given how mutations in this protein complex can lead to unregulated cell growth. These connections reflect the importance of succinic acid in maintaining metabolic stability highlighting its potential as a biomarker in disease contexts.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Functional Studies - Succinate Assay Kit (Colorimetric) (ab204718), expandable thumbnail

    Functional Studies - Succinate Assay Kit (Colorimetric) (ab204718)

    Measurement of Succinate levels in rat liver (40 μg), kidney (40 μg) and muscle (40 μg), Jurkat cell lysate (200 μg) and white wine (4 μl, wine sample was diluted 1:1 with 0.5 M Tris HCl, pH 8.0).

  • Functional Studies - Succinate Assay Kit (Colorimetric) (ab204718), expandable thumbnail

    Functional Studies - Succinate Assay Kit (Colorimetric) (ab204718)

    Succinate Standard Curve.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com