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AB204718

Succinate Assay Kit (Colorimetric)

5

(1 Review)

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(54 Publications)

Succinate Assay Kit (Colorimetric) (ab204718) provides a simple and fast method to quantify succinate levels. Readout on any colorimetric (450 nm) plate reader.

- Cited in over 40 publications
- Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.

View Alternative Names

butanedioic acid, ethane-12 dicarboxylic acid, ethylenesuccinic acid, spirit of amber, succinate

3 Images
Functional Studies - Succinate Assay Kit (Colorimetric) (AB204718)
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Supplier Data

Functional Studies - Succinate Assay Kit (Colorimetric) (AB204718)

Measurement of Succinate levels in rat liver (40 μg), kidney (40 μg) and muscle (40 μg), Jurkat cell lysate (200 μg) and white wine (4 μl, wine sample was diluted 1 : 1 with 0.5 M Tris HCl, pH 8.0).

Functional Studies - Succinate Assay Kit (Colorimetric) (AB204718)
  • FuncS

Supplier Data

Functional Studies - Succinate Assay Kit (Colorimetric) (AB204718)

Succinate Standard Curve.

Schematic Diagram - Succinate Assay Kit (Colorimetric) (AB204718)
  • Schematic Diagram

Supplier Data

Schematic Diagram - Succinate Assay Kit (Colorimetric) (AB204718)

Representative image of Succinate Assay Kit (Colorimetric) ab204718

Components shown from left to right :

- Succinate Converter

- Developer Solution III

- Succinate Substrate Mix

- Succinate Standard

- Enzyme Mix XI

- Assay Buffer 7

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit.

Key facts

Detection method

Colorimetric

Sample types

Tissue, Suspension cells, Adherent cells

Assay type

Quantitative

Sensitivity

= 40 µM

Assay time

30m

Assay Platform

Microplate reader

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Product details

How the assay works

In Succinate Assay Kit protocol, Succinate is utilized by Succinyl-CoA Synthetase to form an intermediate, which undergoes a series of reactions and reduces a colorless probe to a colored product with strong absorbance at OD=450 nm.
Succinate + ATP + CoA converted by succiny-CoA synthase to succiny-CoA + ADP + Pi with Color Detection (OD 450 nm).
This kit has so far only been tested with human and rat samples. Since Succinate is distributed in all plants and animal tissues this kit should detect Succinate independent of the species. Depending on the sample type, the sample preparation might need to be optimized.

Succinate assay protocol summary:

- Add samples and standards to wells
- Add reaction mix and incubate for 30 min
- Analyze with a microplate reader

How other researchers are using

Succinate Assay Kit (Colorimetric)has been used in a variety of sample type including:
- Mouse brain 1
- IPSC-derived cardiomyocytes 2
- Human melanoma (C8161) cells 3
References:

1-Lancaster M et al. 2023
2-Srivastava S et al. 2023
3-Shen L et al. 2023

This product is manufactured by BioVision, an Abcam company and was previously called K649 Succinate (Succinic Acid) Colorimetric Assay Kit. K649-100 is the same size as the 100 test size of ab204718.

Succinic acid (C4H6O4) is distributed in all plants and animal tissues and was first obtained from amber. Due to its low toxicity, it is widely used in agriculture, food and pharmaceutical industry. Succinate (C4H4O4) is the salt or ester of succinic acid. It is one of the most active components of cellular respiratory and intracellular energy generation. Measurement of succinate or succinic acid level is a key to analysis of the citric acid cycle.

This kit has so far only been tested with human and rat samples. Since Succinate is distributed in all plants and animal tissues this kit should detect Succinate independent of the species. Depending on the sample type, the sample preparation might need to be optimized.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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What's included?

{ "values": { "100Test": { "sellingSize": "100 Test", "publicAssetCode":"ab204718-100Test", "assetComponentDetails": [ { "size":"1 x 1 Vial", "name":"Succinate Substrate Mix", "number":"AB204718-CMP06", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Succinate Converter", "number":"AB204718-CMP02", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Enzyme Mix XI", "number":"AB204718-CMP04", "productcode":"" }, { "size":"1 x 25 mL", "name":"Assay Buffer 7", "number":"AB204718-CMP01", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Developer Solution III", "number":"AB204718-CMP03", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Succinate Standard", "number":"AB204718-CMP05", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Succinic acid also known as butanedioic acid plays a significant mechanical role in cellular metabolism. It has a molecular mass of 118.09 g/mol and is important for both prokaryotic and eukaryotic cells. This compound is expressed in mitochondria where it acts as an intermediary in critical metabolic processes. The widespread presence across different types of organisms highlights its essential role in cellular energy production.
Biological function summary

Succinic acid contributes to energy production by participating in the tricarboxylic acid (TCA) cycle also known as the Krebs cycle. It is part of this complex cycle that produces ATP the energy currency of the cell. Succinic acid links different metabolic pathways facilitating the oxidation of nutrients to release energy. This has a broad impact on cellular function and organismal energy homeostasis emphasizing its indispensable role in general metabolism.

Pathways

Succinic acid is heavily involved in the TCA cycle and the electron transport chain. During the TCA cycle it transforms into fumarate through succinate dehydrogenase a vital process for energy extraction in cells. Succinic acid influences the electron transport chain where it facilitates ATP synthesis by interacting with complexes like Coenzyme Q10. These pathways are central to metabolic activity demonstrating how succinic acid integrates into the broader network of cellular respiration.

Succinic acid shows connections with metabolic disorders such as mitochondrial diseases and some cancers. Abnormal accumulation or defective metabolism of succinic acid can lead to mitochondrial dysfunction contributing to disorders like Leigh's syndrome. Additionally its involvement with the succinate dehydrogenase complex indicates potential links to various cancers given how mutations in this protein complex can lead to unregulated cell growth. These connections reflect the importance of succinic acid in maintaining metabolic stability highlighting its potential as a biomarker in disease contexts.
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Product protocols

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Target data

See full target information Succinic acid
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Publications (54)

Recent publications for all applications. Explore the full list and refine your search

International journal of oral science 17:47 PubMed40494898

2025

Succinate modulates oral dysbiosis and inflammation through a succinate receptor 1 dependent mechanism in aged mice.

Applications

Unspecified application

Species

Unspecified reactive species

Fangxi Xu,Yuqi Guo,Scott C Thomas,Anish Saxena,Samantha Hwang,Mridula Vardhan,Xin Li

Cell communication and signaling : CCS 23:189 PubMed40259370

2025

Mannose and PMI depletion overcomes radiation resistance in HPV-negative head and neck cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Tongchuan Wang,Connor Brown,Niamh Doherty,Niall M Byrne,Rayhanul Islam,Meabh Doherty,Jie Feng,Cancan Yin,Sarah Chambers,Lydia McQuoid,Letitia Mohamed-Smith,Karl T Butterworth,Emma M Kerr,Jonathan A Coulter

Frontiers in molecular biosciences 11:1460987 PubMed39297074

2024

Recent progress of methods for cuproptosis detection.

Applications

Unspecified application

Species

Unspecified reactive species

Ligang Zhang,Ruiting Deng,Raoqing Guo,Yawen Jiang,Yichen Guan,Caiyue Chen,Wudi Zhao,Guobin Huang,Lian Liu,Hongli Du,Dongsheng Tang

FEBS letters 598:2592-2614 PubMed39155147

2024

The miR-26a/SIRT6/HIF-1α axis regulates glycolysis and inflammatory responses in host macrophages during Mycobacterium tuberculosis infection.

Applications

Unspecified application

Species

Unspecified reactive species

Soumya Mal,Debayan Majumder,Pankaj Birari,Arun Kumar Sharma,Umesh Gupta,Kuladip Jana,Manikuntala Kundu,Joyoti Basu

Cell communication and signaling : CCS 22:82 PubMed38291428

2024

Extracellular succinate derived from ectopic milieu drives adhesion and implantation growth of ectopic endometrial stromal cells via the SUCNR1 signal in endometriosis.

Applications

Unspecified application

Species

Unspecified reactive species

Qi Tian,Jingyao Ruan,Yuning Wang,Yinping Xiao,Qi Cheng,Yun Chen,Mingqing Li,Kaikai Chang,Xiaofang Yi

Metabolites 14: PubMed38248848

2024

Dysbiotic Vaginal Microbiota Induces Preterm Birth Cascade via Pathogenic Molecules in the Vagina.

Applications

Unspecified application

Species

Unspecified reactive species

AbuZar Ansari,Young-Ah You,Gain Lee,Soo Min Kim,Sun Wha Park,Young Min Hur,Young Ju Kim

Cell reports 42:113241 PubMed37819759

2023

Loss of succinyl-CoA synthetase in mouse forebrain results in hypersuccinylation with perturbed neuronal transcription and metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Makayla S Lancaster,Byungwook Kim,Emma H Doud,Mason D Tate,Ahmad D Sharify,Hongyu Gao,Duojiao Chen,Ed Simpson,Patrick Gillespie,Xiaona Chu,Marcus J Miller,Yue Wang,Yunlong Liu,Amber L Mosley,Jungsu Kim,Brett H Graham

Nature communications 14:4360 PubMed37468519

2023

Nuclear translocation of mitochondrial dehydrogenases as an adaptive cardioprotective mechanism.

Applications

Unspecified application

Species

Unspecified reactive species

Shubhi Srivastava,Priyanka Gajwani,Jordan Jousma,Hiroe Miyamoto,Youjeong Kwon,Arundhati Jana,Peter T Toth,Gege Yan,Sang-Ging Ong,Jalees Rehman

Pathology oncology research : POR 29:1611038 PubMed37351538

2023

CVM-1118 (foslinanib), a 2-phenyl-4-quinolone derivative, promotes apoptosis and inhibits vasculogenic mimicry via targeting TRAP1.

Applications

Unspecified application

Species

Unspecified reactive species

Lifen Shen,Yen-Ling Chen,Chu-Chun Huang,Yu-Chiau Shyu,Richard E B Seftor,Elisabeth A Seftor,Mary J C Hendrix,Du-Shieng Chien,Yi-Wen Chu

Proceedings of the National Academy of Sciences of the United States of America 120:e2217332120 PubMed37253003

2023

Succinyl-CoA ligase ADP-forming subunit beta promotes stress granule assembly to regulate redox and drive cancer metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Austin C Boese,JiHoon Kang,Jung Seok Hwang,Jaehyun Kim,Kiyoung Eun,Courteney M Malin,Kelly R Magliocca,Chaoyun Pan,Lingtao Jin,Sumin Kang
View all publications
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