Succinate Assay Kit (Colorimetric) (ab204718) provides a simple and fast method to quantify succinate levels. Readout on any colorimetric (450 nm) plate reader.
- Cited in over 40 publications
- Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
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- Cell reports 42:1132412023Loss of succinyl-CoA synthetase in mouse forebrain results in hypersuccinylation with perturbed neuronal transcription and metabolism.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMakayla S Lancaster,Byungwook Kim,Emma H Doud,Mason D Tate,Ahmad D Sharify,Hongyu Gao,Duojiao Chen,Ed Simpson,Patrick Gillespie,Xiaona Chu,Marcus J Miller,Yue Wang,Yunlong Liu,Amber L Mosley,Jungsu Kim,Brett H GrahamPubMed 37819759
- Nature communications 14:43602023Nuclear translocation of mitochondrial dehydrogenases as an adaptive cardioprotective mechanism.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShubhi Srivastava,Priyanka Gajwani,Jordan Jousma,Hiroe Miyamoto,Youjeong Kwon,Arundhati Jana,Peter T Toth,Gege Yan,Sang-Ging Ong,Jalees RehmanPubMed 37468519
- Pathology oncology research : POR 29:16110382023CVM-1118 (foslinanib), a 2-phenyl-4-quinolone derivative, promotes apoptosis and inhibits vasculogenic mimicry via targeting TRAP1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLifen Shen,Yen-Ling Chen,Chu-Chun Huang,Yu-Chiau Shyu,Richard E B Seftor,Elisabeth A Seftor,Mary J C Hendrix,Du-Shieng Chien,Yi-Wen ChuPubMed 37351538
- Proceedings of the National Academy of Sciences of the United States of America 120:e22173321202023Succinyl-CoA ligase ADP-forming subunit beta promotes stress granule assembly to regulate redox and drive cancer metastasis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAustin C Boese,JiHoon Kang,Jung Seok Hwang,Jaehyun Kim,Kiyoung Eun,Courteney M Malin,Kelly R Magliocca,Chaoyun Pan,Lingtao Jin,Sumin KangPubMed 37253003
- Frontiers in immunology 14:11967042023Epigenetically altered macrophages promote development of diabetes-associated atherosclerosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDong Huang,Wei Gao,Xin Zhong,Hongxian Wu,You Zhou,Yuanji Ma,Juying Qian,Junbo GePubMed 37215106
- EMBO reports 24:e555482023Reverse electron transfer is activated during aging and contributes to aging and age-related disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSuman Rimal,Ishaq Tantray,Yu Li,Tejinder Pal Khaket,Yanping Li,Sunil Bhurtel,Wen Li,Cici Zeng,Bingwei LuPubMed 36794623
- International journal of molecular sciences 24:2023Tetrathiomolybdate Decreases the Expression of Alkaline Phosphatase in Dermal Papilla Cells by Increasing Mitochondrial ROS Production.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFan Li,Hongli Liu,Xiaojing Wu,Zhicheng Song,Haojia Tang,Maohua Gong,Lei Liu,Fuchang LiPubMed 36834536
- Scientific reports 12:225692022Transcriptome analysis of a dog model of congestive heart failure shows that collagen-related 2-oxoglutarate-dependent dioxygenases contribute to heart failure.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTakahiro Isono,Takehiro Matsumoto,Masafumi Suzaki,Shigehisa Kubota,Susumu Kageyama,Akihiro Kawauchi,Atuyuki WadaPubMed 36581661
- Phytotherapy research : PTR 37:477-4892022Atractylodin targets GLA to regulate D-mannose metabolism to inhibit osteogenic differentiation of human valve interstitial cells and ameliorate aortic valve calcification.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLinghang Qu,Chunli Wang,Haiying Xu,Lanqing Li,Yanju Liu,Qianyun Wan,Kang XuPubMed 36199227
- Cell biology international 46:2207-22192022MicroRNA-16-5p exacerbates sepsis by upregulating aerobic glycolysis via SIRT3-SDHA axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYue-Xian He,Bo-Lun Huang,Yi-Yu Yang,Wen-Xiu Song,Yong-Bo Fan,Li-Mei Zhang,Guo-Sheng LiuPubMed 36153644
Key facts
- Detection method
- Colorimetric
- Sample types
- Tissue, Suspension cells, Adherent cells
- Assay type
- Quantitative
- Reactive species
- Mammals
- Assay time
- 30m
- Sensitivity
- = 40 µM
Target data
Alternative names
butanedioic acid, ethane-12 dicarboxylic acid, ethylenesuccinic acid, spirit of amber, succinate
What's included?
Recommended products
Succinate Assay Kit (Colorimetric) (ab204718) provides a simple and fast method to quantify succinate levels. Readout on any colorimetric (450 nm) plate reader.
- Cited in over 40 publications
- Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
Key facts
- Detection method
- Colorimetric
- Sample types
- Tissue, Suspension cells, Adherent cells
- Assay type
- Quantitative
- Reactive species
- Mammals
- Assay time
- 30m
- Assay Platform
- Microplate reader
- Sensitivity
- = 40 µM
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
How the assay works
In Succinate Assay Kit protocol, Succinate is utilized by Succinyl-CoA Synthetase to form an intermediate, which undergoes a series of reactions and reduces a colorless probe to a colored product with strong absorbance at OD=450 nm.
Succinate + ATP + CoA converted by succiny-CoA synthase to succiny-CoA + ADP + Pi with Color Detection (OD 450 nm).
This kit has so far only been tested with human and rat samples. Since Succinate is distributed in all plants and animal tissues this kit should detect Succinate independent of the species. Depending on the sample type, the sample preparation might need to be optimized.
Succinate assay protocol summary:
- Add samples and standards to wells
- Add reaction mix and incubate for 30 min
- Analyze with a microplate reader
How other researchers are using
Succinate Assay Kit (Colorimetric)has been used in a variety of sample type including:
- Mouse brain 1
- IPSC-derived cardiomyocytes 2
- Human melanoma (C8161) cells 3
References:
1-Lancaster M et al. 2023
2-Srivastava S et al. 2023
3-Shen L et al. 2023
This product is manufactured by BioVision, an Abcam company and was previously called K649 Succinate (Succinic Acid) Colorimetric Assay Kit. K649-100 is the same size as the 100 test size of ab204718.
Succinic acid (C4H6O4) is distributed in all plants and animal tissues and was first obtained from amber. Due to its low toxicity, it is widely used in agriculture, food and pharmaceutical industry. Succinate (C4H4O4) is the salt or ester of succinic acid. It is one of the most active components of cellular respiratory and intracellular energy generation. Measurement of succinate or succinic acid level is a key to analysis of the citric acid cycle.
This kit has so far only been tested with human and rat samples. Since Succinate is distributed in all plants and animal tissues this kit should detect Succinate independent of the species. Depending on the sample type, the sample preparation might need to be optimized.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Succinic acid also known as butanedioic acid plays a significant mechanical role in cellular metabolism. It has a molecular mass of 118.09 g/mol and is important for both prokaryotic and eukaryotic cells. This compound is expressed in mitochondria where it acts as an intermediary in critical metabolic processes. The widespread presence across different types of organisms highlights its essential role in cellular energy production.
- Biological function summary
Succinic acid contributes to energy production by participating in the tricarboxylic acid (TCA) cycle also known as the Krebs cycle. It is part of this complex cycle that produces ATP the energy currency of the cell. Succinic acid links different metabolic pathways facilitating the oxidation of nutrients to release energy. This has a broad impact on cellular function and organismal energy homeostasis emphasizing its indispensable role in general metabolism.
- Pathways
Succinic acid is heavily involved in the TCA cycle and the electron transport chain. During the TCA cycle it transforms into fumarate through succinate dehydrogenase a vital process for energy extraction in cells. Succinic acid influences the electron transport chain where it facilitates ATP synthesis by interacting with complexes like Coenzyme Q10. These pathways are central to metabolic activity demonstrating how succinic acid integrates into the broader network of cellular respiration.
- Associated diseases and disorders
Succinic acid shows connections with metabolic disorders such as mitochondrial diseases and some cancers. Abnormal accumulation or defective metabolism of succinic acid can lead to mitochondrial dysfunction contributing to disorders like Leigh's syndrome. Additionally its involvement with the succinate dehydrogenase complex indicates potential links to various cancers given how mutations in this protein complex can lead to unregulated cell growth. These connections reflect the importance of succinic acid in maintaining metabolic stability highlighting its potential as a biomarker in disease contexts.
Product promise
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Full details and terms and conditions can be found here:
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2 product images
Functional Studies - Succinate Assay Kit (Colorimetric) (ab204718)
Measurement of Succinate levels in rat liver (40 μg), kidney (40 μg) and muscle (40 μg), Jurkat cell lysate (200 μg) and white wine (4 μl, wine sample was diluted 1:1 with 0.5 M Tris HCl, pH 8.0).
Functional Studies - Succinate Assay Kit (Colorimetric) (ab204718)
Succinate Standard Curve.
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