Succinate Dehydrogenase Activity Assay Kit (Colorimetric) ab228560 is a simple and rapid assay for the measurement of Succinate Dehydrogenase activity. Readout on any colorimetric (650 nm) plate reader.
- Kit includes standard curve for quantitation, and SDH enzyme positive control
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- Scientific reports 12:212132022Gum Arabic nanoformulation rescues neuronal lesions in bromobenzene-challenged rats by its antioxidant, anti-apoptotic and cytoprotective potentials.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHailah M Almohaimeed,Hanan Waly,Nasser S Abou Khalil,Khaled M A Hassanein,Basal Sulaiman M Alkhudhairy,Elham A Abd-AllahPubMed 36481816
- Biotechnology and bioengineering 120:409-4252022Selection of synthetic proteins to modulate the human frataxin function.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMaría Florencia Pignataro,María Georgina Herrera,Natalia Brenda Fernández,Martín Aran,Hernán Gustavo Gentili,Fernando Battaglini,Javier SantosPubMed 36225115
- Cell death & disease 13:6602022The hexokinase "HKDC1" interaction with the mitochondria is essential for liver cancer progression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMd Wasim Khan,Alexander R Terry,Medha Priyadarshini,Vladimir Ilievski,Zeenat Farooq,Grace Guzman,Jose Cordoba-Chacon,Issam Ben-Sahra,Barton Wicksteed,Brian T LaydenPubMed 35902556
- Molecular metabolism 58:1014522022Disrupted liver oxidative metabolism in glycine N-methyltransferase-deficient mice is mitigated by dietary methionine restriction.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFerrol I Rome,Curtis C HugheyPubMed 35121169
- Signal transduction and targeted therapy 6:3752021The existence of a nonclassical TCA cycle in the nucleus that wires the metabolic-epigenetic circuitry.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXujun Liu,Wenzhe Si,Lin He,Jianguo Yang,Yani Peng,Jie Ren,Xiaoping Liu,Tong Jin,Huajing Yu,Zihan Zhang,Xiao Cheng,Wenting Zhang,Lu Xia,Yunchao Huang,Yue Wang,Shumeng Liu,Lin Shan,Yu Zhang,Xiaohan Yang,Haixia Li,Jing Liang,Luyang Sun,Yongfeng ShangPubMed 34728602
- Biomedicines 9:2021Endothelial Heme Dynamics Drive Cancer Cell Metabolism by Shaping the Tumor Microenvironment.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSara Petrillo,Francesco De Giorgio,Joanna Kopecka,Tullio Genova,Veronica Fiorito,Anna Lucia Allocco,Francesca Bertino,Deborah Chiabrando,Federico Mussano,Fiorella Altruda,Luca Munaron,Chiara Riganti,Emanuela TolosanoPubMed 34829786
- Gastroenterology 161:1982-1997.e112021Hepatic miR-144 Drives Fumarase Activity Preventing NRF2 Activation During Obesity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesValerio Azzimato,Ping Chen,Emelie Barreby,Cecilia Morgantini,Laura Levi,Ana Vankova,Jennifer Jager,André Sulen,Marina Diotallevi,Joanne X Shen,Anne Miller,Ewa Ellis,Mikael Rydén,Erik Näslund,Anders Thorell,Volker M Lauschke,Keith M Channon,Mark J Crabtree,Arvand Haschemi,Siobhan M Craige,Mattia Mori,Francesco Spallotta,Myriam AouadiPubMed 34425095
- The Prostate 81:1135-11482021SIRT3 affects mitochondrial metabolic reprogramming via the AMPK-PGC-1α axis in the development of benign prostatic hyperplasia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYongzhi Li,Qian Wang,Jingyu Li,Benkang Shi,Yili Liu,Ping WangPubMed 34411320
- Cell reports 35:1092522021The heme synthesis-export system regulates the tricarboxylic acid cycle flux and oxidative phosphorylation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesVeronica Fiorito,Anna Lucia Allocco,Sara Petrillo,Elena Gazzano,Simone Torretta,Saverio Marchi,Francesca Destefanis,Consiglia Pacelli,Valentina Audrito,Paolo Provero,Enzo Medico,Deborah Chiabrando,Paolo Ettore Porporato,Carlotta Cancelliere,Alberto Bardelli,Livio Trusolino,Nazzareno Capitanio,Silvia Deaglio,Fiorella Altruda,Paolo Pinton,Simone Cardaci,Chiara Riganti,Emanuela TolosanoPubMed 34133926
- Nature communications 12:34282021Low chorionic villous succinate accumulation associates with recurrent spontaneous abortion risk.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiao-Hui Wang,Sha Xu,Xiang-Yu Zhou,Rui Zhao,Yan Lin,Jing Cao,Wei-Dong Zang,Hui Tao,Wei Xu,Ming-Qing Li,Shi-Min Zhao,Li-Ping Jin,Jian-Yuan ZhaoPubMed 34103526
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell culture extracts, Tissue Homogenate
- Assay type
- Enzyme activity (quantitative)
- Sensitivity
- < 0.1 mU/well
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Target data
Function
Flavoprotein (FP) subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q) (PubMed:10746566, PubMed:24781757). SDH also oxidizes malate to the non-canonical enol form of oxaloacetate, enol-oxaloacetate (By similarity). Enol-oxaloacetate, which is a potent inhibitor of the succinate dehydrogenase activity, is further isomerized into keto-oxaloacetate (By similarity). Can act as a tumor suppressor (PubMed:20484225).
Alternative names
SDH2, SDHF, SDHA, Flavoprotein subunit of complex II, Malate dehydrogenase [quinone] flavoprotein subunit, Fp
What's included?
Recommended products
Succinate Dehydrogenase Activity Assay Kit (Colorimetric) ab228560 is a simple and rapid assay for the measurement of Succinate Dehydrogenase activity. Readout on any colorimetric (650 nm) plate reader.
- Kit includes standard curve for quantitation, and SDH enzyme positive control
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell culture extracts, Tissue Homogenate
- Assay type
- Enzyme activity (quantitative)
- Assay Platform
- Microplate reader
- Sensitivity
- < 0.1 mU/well
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
How the assay works
In Succinate Dehydrogenase Activity Assay Kit (Colorimetric), Succinate Dehydrogenase converts succinate to fumarate, and transfers the electron to an artificial electron acceptor (Probe), which changes the color from blue to a colorless product (depending upon the sample enzymatic activity).
Succinate Dehydrogenase Activity Assay Kit (Colorimetric) protocol summary:
- Rapidly homogenize sample tissue / cells in assay buffer
- Centrifuge sample and transfer supernatant to fresh tube
- Add sample to plate well
- Add reaction buffer and mix well
- Immediately measure absorbance (600 nm) in kinetic mode for 10 –30 minutes at 25°CThe kit provides enough reagents for 100 assays.
How other researchers are using
Succinate Dehydrogenase Activity Assay Kit (Colorimetric) has been used in a variety of sample type including:
- Mouse liver mitochondria 1
- Rat brain tissues 2
- HepG2 and MCF-7 cells 3
References: 1-Rome F al. 2022
2 Almohaimeed H et al. 2022
3- Liu X et al. 2021
This product is manufactured by BioVision, an Abcam company and was previously called K660 Succinate Dehydrogenase Activity Colorimetric Assay Kit. K660-100 is the same size as the 100 test size of ab228560.
Succinate Dehydrogenase (SDH) (EC 1.3.5.1) or succinate-coenzyme Q reductase (SQR) or respiratory complex II is an enzyme complex, which is bound to the inner mitochondrial membrane. SDH participates in both the citric acid cycle and electron transport chain. In mammals and many bacteria, SDH consists of 2 hydrophilic subunits, SDHA (flavoprotein) and SDHB (iron-sulfur protein) and 2 hydrophobic membrane anchor subunits: SDHC and SDHD. SDH oxidizes succinate to fumarate and transfers the electrons to ubiquinone. SDH deficiency in humans leads to a variety of phenotypes including Leigh syndrome, a neurometabolic disorder, tumor formation, and myopathy. Recent studies show that SDH can prevent the generation of ROS (reactive oxygen species); therefore, measurement of succinate dehydrogenase activity has wide applications.
The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Complex II also known as succinate dehydrogenase (SDH) or succinate-ubiquinone oxidoreductase plays an important mechanical role in the mitochondrial electron transport chain. It catalyzes the oxidation of succinate to fumarate while reducing ubiquinone to ubiquinol. The enzyme complex is approximately 140 kDa in mass and resides in the inner mitochondrial membrane. Complex II is expressed in most tissues particularly in high-energy demand tissues such as the heart and skeletal muscles.
- Biological function summary
Complex II functions as part of the larger electron transport chain complex and plays a role in the Krebs cycle. It links two critical metabolic pathways converting succinate to fumarate while transferring electrons to the electron transport chain. This makes it integral for proper cellular respiration and energy production. The complex consists of multiple subunits and utilizes co-factors like FAD and iron-sulfur clusters for enzymatic activity. It is also a part of the supercomplexes that optimize the efficiency of oxidative phosphorylation.
- Pathways
Complex II plays a significant role in both the citric acid cycle and oxidative phosphorylation. It acts as a connecting bridge between these two pathways facilitating the flow of electrons. Complex II works alongside other proteins such as complex I and complex III to maintain the electron transport chain's function and energy production. Succinate dehydrogenase transfers electrons within the chain directly affecting the generation of ATP by complex V (ATP synthase).
- Associated diseases and disorders
Dysfunction of complex II is associated with mitochondrial diseases and cancers. Mutations or deficiencies in its subunits can lead to conditions like Leigh syndrome and hereditary paraganglioma. These conditions frequently involve other mitochondrial proteins and complexes such as complex I which can exacerbate the electron transport chain dysfunction. In cancers alterations in succinate dehydrogenase activity can result in oncogenic metabolisms by falsely stabilizing hypoxia-inducible factors linking it further with the genetic and metabolic regulation.
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Functional Studies - Succinate Dehydrogenase Activity Assay Kit (Colorimetric) (ab228560)
Typical standard curve and assay data.
(a) DCIP standard curve, (b) typical assay data and (c) succinate dehydrogenase activity in mitochondria isolated from mouse heart (24 μg) and liver (70 μg).
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