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AB83387

Sucrose Assay Kit

5

(1 Review)

|

(2 Publications)

Sucrose Assay Kit ab83387 provides a convenient means for specifically measuring sucrose levels from various biological samples.

Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
2 Images
Functional Studies - Sucrose Assay Kit (AB83387)
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Supplier Data

Functional Studies - Sucrose Assay Kit (AB83387)

Standard Sucrose Assay. Different amounts of sucrose, lactose and maltose are assayed following the kit protocol. Data show that the Sucrose assay kit specifically detects sucrose, but not lactose or maltose.

Schematic Diagram - Sucrose Assay Kit (AB83387)
  • Schematic Diagram

Supplier Data

Schematic Diagram - Sucrose Assay Kit (AB83387)

Representative image of Sucrose Assay Kit ab83387

Components shown from left to right :

- OxiRed Probe

- Invertase

- Sucrose Standard

- Developer Mix B

- Assay Buffer 2

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit.

Key facts

Detection method

Colorimetric/Fluorometric

Sample types

Urine, Plasma, Tissue Extracts, Cell culture supernatant, Serum, Other biological fluids

Results type

Quantitative

Sensitivity

> 0.2 µM

Range

0.0002 - 10 mM

Assay time

1h

Assay Platform

Microplate reader

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Product details

Sucrose Assay Kit ab83387 provides a convenient means for specifically measuring sucrose levels from various biological samples (e.g. serum, plasma, body fluids, food, growth medium, etc.). Sucrose is converted to glucose and fructose by invertase. The generated glucose is then specifically oxidized to generate a product which reacts with a probe to generate color (λ= 570 nm) and fluorescence (Ex/Em = 535/587 nm). Use of proper controls allows for correcting free glucose background. Other disaccharides such as lactose and maltose do not interfere with the assay. The method can detect 0.0002-10 mM sucrose concentrations.

Other Notes
This product was previously called K626 Biovision Sucrose Colorimetric/Fluorometric Assay Kit. Biovision was acquired by Abcam in 2021.

Sucrose (C12H22O11; FW:342.3), also called table sugar, is one of the most important fuel sources to generate the universal energy molecule ATP. Sucrose is a disaccharide which can be converted into one glucose and one fructose.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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What's included?

{ "values": { "100Test": { "sellingSize": "100 Test", "publicAssetCode":"ab83387-100Test", "assetComponentDetails": [ { "size":"1 x 100 µL", "name":"Sucrose Standard", "number":"AB83387-CMP05", "productcode":"" }, { "size":"1 x 0.2 mL", "name":"OxiRed™ Probe", "number":"AB83387-CMP03", "productcode":"AB309437" }, { "size":"1 x 1 Vial", "name":"Invertase", "number":"AB83387-CMP01", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Developer Mix B", "number":"AB83387-CMP02", "productcode":"AB154151" }, { "size":"1 x 25 mL", "name":"Assay Buffer 2", "number":"AB83387-CMP04", "productcode":"AB136652" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Product protocols

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Target data

See full target information Sucrose
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Biopharmaceutics & drug disposition 44:71-83 PubMed35508078

2022

Optimizing transcardial perfusion of small molecules and biologics for brain penetration and biodistribution studies in rodents.

Applications

Unspecified application

Species

Unspecified reactive species

Keumhan Noh,Xingrong Liu,Cong Wei

Bioresource technology 272:1-9 PubMed30292911

2018

Production of various organic acids from different renewable sources by immobilized cells in the regimes of separate hydrolysis and fermentation (SHF) and simultaneous saccharification and fermentation (SFF).

Applications

Unspecified application

Species

Unspecified reactive species

Olga Maslova,Nikolay Stepanov,Olga Senko,Elena Efremenko
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com