Sucrose Assay Kit ab83387 provides a convenient means for specifically measuring sucrose levels from various biological samples.
Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
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- Biopharmaceutics & drug disposition 44:71-832022Optimizing transcardial perfusion of small molecules and biologics for brain penetration and biodistribution studies in rodents.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKeumhan Noh,Xingrong Liu,Cong WeiPubMed 35508078
- Bioresource technology 272:1-92018Production of various organic acids from different renewable sources by immobilized cells in the regimes of separate hydrolysis and fermentation (SHF) and simultaneous saccharification and fermentation (SFF).Applications:Unspecified applicationReactive species:Unspecified reactive speciesOlga Maslova,Nikolay Stepanov,Olga Senko,Elena EfremenkoPubMed 30292911
Key facts
- Detection method
- Colorimetric/Fluorometric
- Sample types
- Urine, Plasma, Tissue Extracts, Cell culture supernatant, Serum, Other biological fluids
- Assay type
- Quantitative
- Range
- 0.0002 - 10 mM
- Assay time
- 1h
- Sensitivity
- > 0.2 µM
Target data
What's included?
Recommended products
Sucrose Assay Kit ab83387 provides a convenient means for specifically measuring sucrose levels from various biological samples.
Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
Key facts
- Detection method
- Colorimetric/Fluorometric
- Sample types
- Urine, Plasma, Tissue Extracts, Cell culture supernatant, Serum, Other biological fluids
- Assay type
- Quantitative
- Range
- 0.0002 - 10 mM
- Assay time
- 1h
- Assay Platform
- Microplate reader
- Sensitivity
- > 0.2 µM
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
Sucrose Assay Kit ab83387 provides a convenient means for specifically measuring sucrose levels from various biological samples (e.g. serum, plasma, body fluids, food, growth medium, etc.). Sucrose is converted to glucose and fructose by invertase. The generated glucose is then specifically oxidized to generate a product which reacts with a probe to generate color (λ= 570 nm) and fluorescence (Ex/Em = 535/587 nm). Use of proper controls allows for correcting free glucose background. Other disaccharides such as lactose and maltose do not interfere with the assay. The method can detect 0.0002-10 mM sucrose concentrations.
Other Notes
This product was previously called K626 Biovision Sucrose Colorimetric/Fluorometric Assay Kit. Biovision was acquired by Abcam in 2021.
Sucrose (C12H22O11; FW:342.3), also called table sugar, is one of the most important fuel sources to generate the universal energy molecule ATP. Sucrose is a disaccharide which can be converted into one glucose and one fructose.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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Functional Studies - Sucrose Assay Kit (ab83387)
Standard Sucrose Assay. Different amounts of sucrose, lactose and maltose are assayed following the kit protocol. Data show that the Sucrose assay kit specifically detects sucrose, but not lactose or maltose.
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Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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