Sucrose Assay Kit ab83387 provides a convenient means for specifically measuring sucrose levels from various biological samples.
Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
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- Biopharmaceutics & drug disposition 44:71-832022Optimizing transcardial perfusion of small molecules and biologics for brain penetration and biodistribution studies in rodents.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKeumhan Noh,Xingrong Liu,Cong WeiPubMed 35508078
- Bioresource technology 272:1-92018Production of various organic acids from different renewable sources by immobilized cells in the regimes of separate hydrolysis and fermentation (SHF) and simultaneous saccharification and fermentation (SFF).Applications:Unspecified applicationReactive species:Unspecified reactive speciesOlga Maslova,Nikolay Stepanov,Olga Senko,Elena EfremenkoPubMed 30292911
Key facts
- Detection method
Colorimetric/Fluorometric
- Sample types
Urine, Plasma, Tissue Extracts, Cell culture supernatant, Serum, Other biological fluids
- Assay type
Quantitative
- Range
0.0002 - 10 µM
- Assay time
1h
- Sensitivity
> 0.2 µM
Target data
What's included?
Recommended products
Sucrose Assay Kit ab83387 provides a convenient means for specifically measuring sucrose levels from various biological samples.
Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
Key facts
- Detection method
Colorimetric/Fluorometric
- Sample types
Urine, Plasma, Tissue Extracts, Cell culture supernatant, Serum, Other biological fluids
- Assay type
Quantitative
- Range
0.0002 - 10 µM
- Assay time
1h
- Assay Platform
Microplate reader
- Sensitivity
> 0.2 µM
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
-20°C
- Appropriate long-term storage conditions
-20°C
- Storage information
-20°C
Notes
Abcam's Sucrose Assay Kit provides a convenient means for specifically measuring sucrose levels from various biological samples (e.g. serum, plasma, body fluids, food, growth medium, etc.). Sucrose is converted to glucose and fructose by invertase. The generated glucose is then specifically oxidized to generate a product which reacts with a probe to generate color (λ= 570 nm) and fluorescence (Ex/Em = 535/587 nm). Use of proper controls allows for correcting free glucose background. Other disaccharides such as lactose and maltose do not interfere with the assay. The method can detect 0.0002-10 mM sucrose concentrations.
Visit our FAQs page for tips and troubleshooting.
This product is manufactured by BioVision, an Abcam company and was previously called K626 Sucrose Colorimetric/Fluorometric Assay Kit. K626-100 is the same size as the 100 test size of ab83387.
Sucrose (C12H22O11; FW:342.3), also called table sugar, is one of the most important fuel sources to generate the universal energy molecule ATP. Sucrose is a disaccharide which can be converted into one glucose and one fructose.
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1 product image
Functional Studies - Sucrose Assay Kit (ab83387)
Standard Sucrose Assay. Different amounts of sucrose, lactose and maltose are assayed following the kit protocol. Data show that the Sucrose assay kit specifically detects sucrose, but not lactose or maltose.
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Product protocols
- Visit the general protocols
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