Sulfatase Activity Assay Kit (Colorimetric)
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Supplier Data
Functional Studies - Sulfatase Activity Assay Kit (Colorimetric) (AB204731)
Measurement of Sulfatase Activity in HepG2 cell lysate (30 μg) and rat liver homogenate (100 μg).
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Supplier Data
Functional Studies - Sulfatase Activity Assay Kit (Colorimetric) (AB204731)
Enzyme activity measuring different amounts of the provided Sulfatase.
- FuncS
Supplier Data
Functional Studies - Sulfatase Activity Assay Kit (Colorimetric) (AB204731)
Typical 4-Nitrocatechol Standard calibration curve.
Reactivity data
Product details
Sulfatase Activity Assay Kit (ab204731) provides a quick and easy way to determine sulfatase activity. The kit measures the hydrolysis of a sulfate ester to 4-nitrocatechol, which can be detected at OD=515 nm. The kit is suitable for measuring activity of purified enzyme as well as sulfatase from biological samples. The limit of detection is below 1 mU.
Other Notes
This product was previously called K675 Biovision Sulfatase Activity Assay Kit (Colorimetric). Biovision was acquired by Abcam in 2021.
Sulfatases (EC 3.1.6) are enzymes in the esterase class that catalyze the hydrolysis of sulfate esters from a wide range of biological molecules, including steroids, carbohydrates, and proteins. They can be found in intracellular and extracellular spaces and are distributed in a wide range of cells and tissues. Intracellular sulfatases are commonly found localized within the lysosome. Genetic defects in sulfatase can result in certain lysosomal storage disorders and abnormal expression can contribute to certain hormone-dependent cancers, such as breast and prostate cancer.
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This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Sulfatases participate in essential cellular processes such as hormone regulation cell signaling and the degradation of glycosaminoglycans. The enzymes exist as a part of a larger group of proteins linked to the metabolism of sulfated compounds. These functions make them indispensable for numerous physiological activities including modification and breakdown of sulfate group-bearing molecules. Sulfatases have a critical role in maintaining homeostasis by altering the charge and solubility of biologically essential compounds.
Pathways
Sulfatases integrate into key biological pathways like the lysosomal degradation pathway and the steroid biosynthesis pathway. In lysosomal degradation sulfatases help break down complex carbohydrates that are otherwise difficult to process. In steroid biosynthesis they modify steroid sulfates back into active steroids influencing hormonal balance. The pathway involves other proteins such as estrogen sulfotransferases which work collaboratively with sulfatases to regulate hormonal actions.
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