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Sulfatase Activity Assay Kit (ab204731) provides a quick and easy way to determine sulfatase activity.

Is this your first time purchasing this assay kit? Get 20% off one kit by entering code 20OFF-T5A8Q at checkout. T&C’s apply – click here. Offer only valid when purchasing directly from Abcam in Europe, North America, Australia, and Singapore.

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Key facts

Detection method

Colorimetric

Sample types

Tissue, Suspension cells, Adherent cells

Assay type

Enzyme activity

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What's included?

100 Test
Components
4-Nitrocatechol Standard
1 x 1.5 mL
Stop Solution VI
1 x 10 mL
Sulfatase Assay Buffer
1 x 5 mL
Sulfatase Enzyme Mix
1 x 1 Vial
Sulfatase Substrate
1 x 4 mL

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Sulfatase Activity Assay Kit (ab204731) provides a quick and easy way to determine sulfatase activity.

Is this your first time purchasing this assay kit? Get 20% off one kit by entering code 20OFF-T5A8Q at checkout. T&C’s apply – click here. Offer only valid when purchasing directly from Abcam in Europe, North America, Australia, and Singapore.

Key facts

Detection method

Colorimetric

Sample types

Tissue, Suspension cells, Adherent cells

Assay type

Enzyme activity

Assay Platform

Microplate reader

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

-20°C

Notes

Sulfatase Activity Assay Kit (ab204731) provides a quick and easy way to determine sulfatase activity. The kit measures the hydrolysis of a sulfate ester to 4-nitrocatechol, which can be detected at OD=515 nm. The kit is suitable for measuring activity of purified enzyme as well as sulfatase from biological samples. The limit of detection is below 1 mU.

This product is manufactured by BioVision, an Abcam company and was previously called K675 Sulfatase Activity Assay Kit (Colorimetric). K675-100 is the same size as the 100 test size of ab204731.

Sulfatases (EC 3.1.6) are enzymes in the esterase class that catalyze the hydrolysis of sulfate esters from a wide range of biological molecules, including steroids, carbohydrates, and proteins. They can be found in intracellular and extracellular spaces and are distributed in a wide range of cells and tissues. Intracellular sulfatases are commonly found localized within the lysosome. Genetic defects in sulfatase can result in certain lysosomal storage disorders and abnormal expression can contribute to certain hormone-dependent cancers, such as breast and prostate cancer.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Functional Studies - Sulfatase Activity Assay Kit (Colorimetric) (ab204731), expandable thumbnail

    Functional Studies - Sulfatase Activity Assay Kit (Colorimetric) (ab204731)

    Typical 4-Nitrocatechol Standard calibration curve.

  • Functional Studies - Sulfatase Activity Assay Kit (Colorimetric) (ab204731), expandable thumbnail

    Functional Studies - Sulfatase Activity Assay Kit (Colorimetric) (ab204731)

    Enzyme activity measuring different amounts of the provided Sulfatase.

  • Functional Studies - Sulfatase Activity Assay Kit (Colorimetric) (ab204731), expandable thumbnail

    Functional Studies - Sulfatase Activity Assay Kit (Colorimetric) (ab204731)

    Measurement of Sulfatase Activity in HepG2 cell lysate (30 μg) and rat liver homogenate (100 μg).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

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