Superoxide Dismutase Activity Assay Kit (Colorimetric) ab65354 is a simple and rapid assay for superoxide dismutase (SOD) activity.
Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
Colorimetric
Urine, Plasma, Tissue Extracts, Cell culture media, Serum, Other biological fluids, Cell Lysate
Enzyme activity
Mammals
30m
Destroys radicals which are normally produced within the cells and which are toxic to biological systems.
SOD2, SOD3
Superoxide dismutase [Cu-Zn], Superoxide dismutase 1, hSod1, SOD1
Superoxide Dismutase Activity Assay Kit (Colorimetric) ab65354 is a simple and rapid assay for superoxide dismutase (SOD) activity.
Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
Superoxide dismutase [Cu-Zn], Superoxide dismutase 1, hSod1, SOD1
Colorimetric
Urine, Plasma, Tissue Extracts, Cell culture media, Serum, Other biological fluids, Cell Lysate
Enzyme activity
Mammals
30m
Microplate reader
Blue Ice
-20°C
-20°C
Please refer to protocols
Superoxide Dismutase Activity Assay Kit (Colorimetric) ab65354 is a simple and rapid assay for superoxide dismutase (SOD) activity.
In the SOD assay protocol:
- superoxide anions are produced by the action of xanthine oxidase
- SOD catalyzes the dismutation of the superoxide anion into hydrogen peroxide and O2
- superoxide anions act on WST-1 to produce a water-soluble formazan dye which can be detected by the increase in absorbance at 450 nm
The greater the activity of SOD in the sample, the less formazan dye is produced.
Superoxide dismutase assay protocol summary:
- add samples to wells
- add WST-1 working solution and enzyme working solution and incubate for 20 min at 37°C
- analyze with microplate reader
Superoxide dismutase (SOD) is one of the most important antioxidative enzymes. It catalyzes the dismutation of the superoxide anion into hydrogen peroxide and molecular oxygen. **Related products** Review the to learn about more assays for oxidative stress.
This supplementary information is collated from multiple sources and compiled automatically.
Superoxide dismutase also called SOD functions as an antioxidant enzyme that converts superoxide radicals into oxygen and hydrogen peroxide. It plays a critical role in reducing oxidative stress in cells. The enzyme exists in several isoforms including SOD1 SOD2 and SOD3 each with different cellular localizations and cofactors. SOD1 or cytosolic copper-zinc superoxide dismutase has a mass of approximately 32 kDa and is found in the cytoplasm. SOD2 known as manganese superoxide dismutase is mitochondrial and shares similar antioxidant functions. SOD3 is extracellular and has a distinct expression pattern mostly found in tissues like lungs and blood vessels.
SOD enzymes protect cells by dismutating reactive superoxide radicals preventing cellular damage and apoptosis. These enzymes although not forming large protein complexes have critical interactions with cellular systems maintaining redox balance. Superoxide radicals if not regulated can lead to lipid peroxidation DNA damage and protein oxidation disrupting normal cellular processes. Superoxide dismutases thereby hold a defensive position in maintaining physiological homeostasis.
Superoxide dismutase enzymes especially within the reactive oxygen species (ROS) signaling pathway contribute to cellular defense mechanisms. In the antioxidant defense pathway SOD enzymes act alongside catalase and glutathione peroxidase. They modulate oxidative stress levels that play a signal transduction role in processes like cell proliferation and apoptosis. This activity links them with proteins like catalase which further catabolize hydrogen peroxide into water and oxygen completing the detoxification process initiated by SOD.
Superoxide dismutases are associated with neurodegenerative diseases and cancer. Mutations in SOD1 have links to amyotrophic lateral sclerosis (ALS) where misfolded proteins lead to motor neuron degeneration. SOD activity levels altered oxidative stress responses and faulty mitochondrial functions connect with Alzheimer's disease. The disease associations also include seemingly related proteins like TDP-43 in ALS pointing towards a wider network of dysfunction in oxidative stress pathways.
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Park J et al investigates the recovery in erectile function after administration of chronic statin alone in DM (streptozotocin (STZ)-induced diabetes mellitus) rats. SOD activity was determined using Superoxide Dismutase activity assay kit (ab65354).
* Indicates statistical significance in comparison with DM group (P < 0.05). # Indicates statistical significance in comparison with the statin group (P<0.05).
Superoxide dismutase measured in biofluids at various dilutions
Superoxidase dismutase (ab90040) measured showing inhibition rate (%) per concentration (microgram per mL)
Principle of Superoxide Dismutase Assay.
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