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Superoxide Dismutase Activity Assay Kit (Colorimetric) ab65354 is a simple and rapid assay for superoxide dismutase (SOD) activity.

Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.


Images

Functional Studies - Superoxide Dismutase Activity Assay Kit (Colorimetric) (AB65354), expandable thumbnail
  • Functional Studies - Superoxide Dismutase Activity Assay Kit (Colorimetric) (AB65354), expandable thumbnail
  • Functional Studies - Superoxide Dismutase Activity Assay Kit (Colorimetric) (AB65354), expandable thumbnail
  • Functional Studies - Superoxide Dismutase Activity Assay Kit (Colorimetric) (AB65354), expandable thumbnail

Publications

Key facts

Detection method

Colorimetric

Sample types

Urine, Plasma, Tissue Extracts, Cell culture media, Serum, Other biological fluids, Cell Lysate

Assay type

Enzyme activity

Reactive species

Mammals

Assay time

30m

Target data

Function

Destroys radicals which are normally produced within the cells and which are toxic to biological systems.

Targets

SOD2, SOD3

Alternative names

What's included?

2000 Test
Components
SOD Assay Buffer
20 x 20 mL
SOD Dilution Buffer
20 x 10 mL
SOD Enzyme Solution
20 x 1 Vial
WST Reagent II
20 x 1 mL

Recommended products

Superoxide Dismutase Activity Assay Kit (Colorimetric) ab65354 is a simple and rapid assay for superoxide dismutase (SOD) activity.

Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.

Alternative names

Key facts

Detection method

Colorimetric

Sample types

Urine, Plasma, Tissue Extracts, Cell culture media, Serum, Other biological fluids, Cell Lysate

Assay type

Enzyme activity

Reactive species

Mammals

Assay time

30m

Assay Platform

Microplate reader

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

Please refer to protocols

Notes

Superoxide Dismutase Activity Assay Kit (Colorimetric) ab65354 is a simple and rapid assay for superoxide dismutase (SOD) activity.

In the SOD assay protocol:
- superoxide anions are produced by the action of xanthine oxidase
- SOD catalyzes the dismutation of the superoxide anion into hydrogen peroxide and O2
- superoxide anions act on WST-1 to produce a water-soluble formazan dye which can be detected by the increase in absorbance at 450 nm
The greater the activity of SOD in the sample, the less formazan dye is produced.

Superoxide dismutase assay protocol summary:
- add samples to wells
- add WST-1 working solution and enzyme working solution and incubate for 20 min at 37°C
- analyze with microplate reader

Superoxide dismutase (SOD) is one of the most important antioxidative enzymes. It catalyzes the dismutation of the superoxide anion into hydrogen peroxide and molecular oxygen. **Related products** Review the to learn about more assays for oxidative stress.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Superoxide dismutase also called SOD functions as an antioxidant enzyme that converts superoxide radicals into oxygen and hydrogen peroxide. It plays a critical role in reducing oxidative stress in cells. The enzyme exists in several isoforms including SOD1 SOD2 and SOD3 each with different cellular localizations and cofactors. SOD1 or cytosolic copper-zinc superoxide dismutase has a mass of approximately 32 kDa and is found in the cytoplasm. SOD2 known as manganese superoxide dismutase is mitochondrial and shares similar antioxidant functions. SOD3 is extracellular and has a distinct expression pattern mostly found in tissues like lungs and blood vessels.

Biological function summary

SOD enzymes protect cells by dismutating reactive superoxide radicals preventing cellular damage and apoptosis. These enzymes although not forming large protein complexes have critical interactions with cellular systems maintaining redox balance. Superoxide radicals if not regulated can lead to lipid peroxidation DNA damage and protein oxidation disrupting normal cellular processes. Superoxide dismutases thereby hold a defensive position in maintaining physiological homeostasis.

Pathways

Superoxide dismutase enzymes especially within the reactive oxygen species (ROS) signaling pathway contribute to cellular defense mechanisms. In the antioxidant defense pathway SOD enzymes act alongside catalase and glutathione peroxidase. They modulate oxidative stress levels that play a signal transduction role in processes like cell proliferation and apoptosis. This activity links them with proteins like catalase which further catabolize hydrogen peroxide into water and oxygen completing the detoxification process initiated by SOD.

Associated diseases and disorders

Superoxide dismutases are associated with neurodegenerative diseases and cancer. Mutations in SOD1 have links to amyotrophic lateral sclerosis (ALS) where misfolded proteins lead to motor neuron degeneration. SOD activity levels altered oxidative stress responses and faulty mitochondrial functions connect with Alzheimer's disease. The disease associations also include seemingly related proteins like TDP-43 in ALS pointing towards a wider network of dysfunction in oxidative stress pathways.

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

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4 product images

  • Functional Studies - Superoxide Dismutase Activity Assay Kit (Colorimetric) (ab65354), expandable thumbnail
    Image from Park J et al., PLoS One 12(2), fig 4b. Doi: 10.1371/journal.pone.0172751 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

    Functional Studies - Superoxide Dismutase Activity Assay Kit (Colorimetric) (ab65354)

    Park J et al investigates the recovery in erectile function after administration of chronic statin alone in DM (streptozotocin (STZ)-induced diabetes mellitus) rats. SOD activity was determined using Superoxide Dismutase activity assay kit (ab65354).

    * Indicates statistical significance in comparison with DM group (P < 0.05). # Indicates statistical significance in comparison with the statin group (P<0.05).

  • Functional Studies - Superoxide Dismutase Activity Assay Kit (Colorimetric) (ab65354), expandable thumbnail

    Functional Studies - Superoxide Dismutase Activity Assay Kit (Colorimetric) (ab65354)

    Superoxide dismutase measured in biofluids at various dilutions

  • Functional Studies - Superoxide Dismutase Activity Assay Kit (Colorimetric) (ab65354), expandable thumbnail

    Functional Studies - Superoxide Dismutase Activity Assay Kit (Colorimetric) (ab65354)

    Superoxidase dismutase (ab90040) measured showing inhibition rate (%) per concentration (microgram per mL)

  • Functional Studies - Superoxide Dismutase Activity Assay Kit (Colorimetric) (ab65354), expandable thumbnail

    Functional Studies - Superoxide Dismutase Activity Assay Kit (Colorimetric) (ab65354)

    Principle of Superoxide Dismutase Assay.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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