Thioredoxin Reductase Assay Kit (ab83463) is a specific assay for detecting Thioredoxin Reductase (TrxR) activity.
Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
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- Nature communications 14:69372023A genome-scale metabolic model of parasitic whipworm.Applications:Unspecified applicationReactive species:Unspecified reactive speciesÖmer F Bay,Kelly S Hayes,Jean-Marc Schwartz,Richard K Grencis,Ian S RobertsPubMed 37907472
- Cell death & disease 13:4492022Energy restriction induced SIRT6 inhibits microglia activation and promotes angiogenesis in cerebral ischemia via transcriptional inhibition of TXNIP.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMing-Yu Song,Fang Yi,Hui Xiao,Jun Yin,Qing Huang,Jian Xia,Xiao-Meng Yin,Yan-Bin Wen,Le Zhang,Yun-Hai Liu,Bo Xiao,Wen-Ping GuPubMed 35562171
- Journal of integrative plant biology 64:771-7862022Regulation of cytokinin biosynthesis using PtRD26 -IPT module improves drought tolerance through PtARR10-PtYUC4/5-mediated reactive oxygen species removal in Populus.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHou-Ling Wang,Qi Yang,Shuya Tan,Ting Wang,Yi Zhang,Yanli Yang,Weilun Yin,Xinli Xia,Hongwei Guo,Zhonghai LiPubMed 34990062
- Biological trace element research 200:2365-23792021Antioxidant Activities and Selenogene Transcription in the European Sea Bass (Dicentrarchus labrax) Liver Depend, in a Non-linear Manner, on the Se/Hg Molar Ratio of the Feeds.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMarinelle Espino,Harkaitz Eguiraun,Oihane Diaz de Cerio,José Antonio Carrero,Nestor Etxebarria,Iciar MartinezPubMed 34331176
- Cancers 13:2021A Novel Benzopyrane Derivative Targeting Cancer Cell Metabolic and Survival Pathways.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDana M Zaher,Wafaa S Ramadan,Raafat El-Awady,Hany A Omar,Fatema Hersi,Vunnam Srinivasulu,Ibrahim Y Hachim,Farah I Al-Marzooq,Cijo G Vazhappilly,Salim Merali,Carmen Merali,Nelson C Soares,Paul Schilf,Saleh M Ibrahim,Taleb H Al-TelPubMed 34200264
- Apoptosis : an international journal on programmed cell death 26:253-2602021Activated p53 in the anti-apoptotic milieu of tuberous sclerosis gene mutation induced diseases leads to cell death if thioredoxin reductase is inhibited.Applications:Unspecified applicationReactive species:Unspecified reactive speciesElHusseiny M M Abdelwahab,Judit Bovari-Biri,Gabor Smuk,Janos Fillinger,Donald McPhail,Vera P Krymskaya,Judit E PongraczPubMed 33860865
- Aging cell 19:e132752020G6PD overexpression protects from oxidative stress and age-related hearing loss.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJose M Bermúdez-Muñoz et. al.PubMed 33222382
- International journal of molecular sciences 21:2020Expression of Thioredoxin/Thioredoxin Reductase System Genes in Aphid-Challenged Maize Seedlings.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHubert Sytykiewicz et. al.PubMed 32878074
- Molecules (Basel, Switzerland) 25:2020Compensatory Neuroprotective Response of Thioredoxin Reductase against Oxidative-Nitrosative Stress Induced by Experimental Autoimmune Encephalomyelitis in Rats: Modulation by Theta Burst Stimulation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesIvana Stevanovic et. al.PubMed 32867364
- Translational oncology 13:1007852020Redox Modulation and Induction of Ferroptosis as a New Therapeutic Strategy in Hepatocellular Carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJana Lippmann et. al.PubMed 32416440
Key facts
- Detection method
Colorimetric
- Sample types
Cell culture extracts, Tissue Extracts, Other biological fluids, Serum
- Assay type
Enzyme activity
- Assay time
40m
Target data
Function
Reduces disulfideprotein thioredoxin (Trx) to its dithiol-containing form (PubMed:8577704). Homodimeric flavoprotein involved in the regulation of cellular redox reactions, growth and differentiation. Contains a selenocysteine residue at the C-terminal active site that is essential for catalysis (Probable). Also has reductase activity on hydrogen peroxide (H2O2) (PubMed:10849437).Isoform 1Induces actin and tubulin polymerization, leading to formation of cell membrane protrusions.Isoform 4Enhances the transcriptional activity of estrogen receptors ESR1 and ESR2.Isoform 5Enhances the transcriptional activity of the estrogen receptor ESR2 only (PubMed:15199063). Mediates cell death induced by a combination of interferon-beta and retinoic acid (PubMed:9774665).
Targets
TXNRD2, TXNRD3
Alternative names
TR, Gene associated with retinoic and interferon-induced mortality 12 protein, KM-102-derived reductase-like factor, Peroxidase TXNRD1, Thioredoxin reductase TR1, GRIM-12, Gene associated with retinoic and IFN-induced mortality 12 protein, KDRF, GRIM12, TXNRD1
What's included?
Recommended products
Thioredoxin Reductase Assay Kit (ab83463) is a specific assay for detecting Thioredoxin Reductase (TrxR) activity.
Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
Alternative names
TR, Gene associated with retinoic and interferon-induced mortality 12 protein, KM-102-derived reductase-like factor, Peroxidase TXNRD1, Thioredoxin reductase TR1, GRIM-12, Gene associated with retinoic and IFN-induced mortality 12 protein, KDRF, GRIM12, TXNRD1
Key facts
- Detection method
Colorimetric
- Sample types
Cell culture extracts, Tissue Extracts, Other biological fluids, Serum
- Assay type
Enzyme activity
- Assay time
40m
- Assay Platform
Microplate reader
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
-20°C
- Appropriate long-term storage conditions
-20°C
- Storage information
-20°C
Notes
Thioredoxin Reductase Assay Kit (ab83463) is a specific assay for detecting Thioredoxin Reductase (TrxR) activity.
In the thioredoxin reductase assay protocol, TrxR catalyzes the reduction of DTNB to TNB2- in the presence of NADPH, which generates a strong yellow color (ODmax = 412 nm).
Other enzymes present in crude biological samples such as glutathione reductase and glutathione peroxidase can also reduce DTNB. In order to measure TrxR-only activity, a TrxR specific inhibitor is used in a separate reaction to determine TrxR specific activity. The difference between total DTNB reduction in the sample and DTNM reduction in the sample in presence of TrxR inhibitor is the value of specific TrxR activity in the sample.
We have tested the GR positive control from Glutathione Reductase (GR) Assay Kit ab83461 in the conditions of ab83463, and we do not observe activity of the GR positive control.
Thioredoxin reductase assay protocol summary:
- add samples and standards to wells
- add reaction mix
- analyze with a microplate reader over 20 min
This product is manufactured by BioVision, an Abcam company and was previously called K763 Thioredoxin Reductase Activity Colorimetric Assay Kit. K763-100 is the same size as the 100 test size of ab83463.
Thioredoxin reductase (TrxR, EC 1.8.1.9) is a ubiquitous mammalian enzyme that catalyzes the NADPH-dependent reduction of the redox protein thioredoxin, as well as of other endogenous and exogenous compounds such as selenite, lipid hydroperoxides and hydrogen peroxide.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Thioredoxin reductase also known as TrxR or TXNRD is an enzyme that reduces thioredoxin and other substrates. It is a selenoprotein with a molecular mass of approximately 55 kDa and contains a significant selenocysteine residue at its active site. Thioredoxin reductase exists in cytosol mitochondria and sometimes in the endoplasmic reticulum across various cell types allowing it to fulfill its role in maintaining cellular redox homeostasis by facilitating the reduction of thioredoxin.
- Biological function summary
Thioredoxin reductase participates in several critical cellular processes beyond just reducing substrates. It acts as an important player in antioxidant defense cellular signaling and apoptosis. As part of the thioredoxin system which often works closely with other antioxidant systems it plays a role in detoxifying harmful reactive oxygen species. It is not typically part of larger protein complexes but interacts functionally with many proteins to regulate redox signaling pathways.
- Pathways
Thioredoxin reductase engages in the regulation of redox signaling and is active in the thioredoxin system pathway. This pathway plays an important role in controlling enzymatic processes that guard cells against oxidative stress. Thioredoxin reductase is also involved in the NADPH-dependent thioredoxin system alongside thioredoxin and NADPH. The enzyme is correlated with the activity of glutathione peroxidase emphasizing its role in redox balance within the cell.
- Associated diseases and disorders
Thioredoxin reductase is implicated in the progression of cancer and cardiovascular diseases. Its overexpression is often observed in cancerous cells where it aids in cell survival and proliferation by managing oxidative stress levels. In cardiovascular diseases altered thioredoxin reductase activity relates to endothelial dysfunction. The enzyme interacts with proteins like thioredoxin to impact disease mechanisms highlighting its importance in therapeutic targets for these conditions.
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5 product images
Image from Bulman CA et al., PLoS One. 2015;9(2):e0003534. Fig 4(A).; doi: 10.1371/journal.pntd.0003534. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/Functional Studies - Thioredoxin Reductase Assay Kit (Colorimetric) (ab83463)
Activity of endogenous Brugia thioredoxin reductase from soluble worm lysates following incubation with 1% DMSO or 0.3 μM, 0.1 μM, or 0.03 μM of auranofin in vitro. Percentages indicate the percent activity of TrxR compared to DMSO controls. Thioredoxin reductase activity was significantly reduced (p < 0.05) to 15%, 33% and 69% of endogenous activity, respectively, compared to the activity in DMSO-treated worms.
Thioredoxin reductase activity of worm lysates was assayed using female B. malayi treated in vitro with either 0.3 μM, 0.1 μM, or 0.03 μM auranofin or 1% DMSO. After 5 hours of treatment, worm motility was measured using the Worminator, and then worms (24 in each group) were pooled, washed three times in PBS, and lysed by douncing in a glass homogenizer in assay buffer (ab83463) with 1 mM PMSF. The crude lysates were centrifuged at 10,000 rcf for 15 minutes at 4°C to pellet insoluble material. The total protein concentrations of soluble lysates were measured using the Bradford assay. The soluble lysates were incubated for 20 minutes in assay buffer or assay buffer with a proprietary thioredoxin reductase specific inhibitor before adding a specific substrate, DTNB (5, 5′-dithiobis (2-nitrobenzoic) acid), and measuring activity at 20 second intervals for 40 minutes using the SpectraMax Plus Microplate Reader (Molecular Devices, Sunnyvale, CA) at λ = 412 nm. Lysates were tested in duplicate. TrxR activity was calculated based on the linear amount of TNB produced per minute per mg of total protein and adjusted for background activity from enzymes other than TrxR in the lysates.
Functional Studies - Thioredoxin Reductase Assay Kit (Colorimetric) (ab83463)
Thioredoxin reductase measured in mouse tissue lysates showing activity (mU) per mg protein of sample tested
Functional Studies - Thioredoxin Reductase Assay Kit (Colorimetric) (ab83463)
Thioredoxin reductase measured in cell lysates showing activity (mU) per 1 mln of cells tested
Functional Studies - Thioredoxin Reductase Assay Kit (Colorimetric) (ab83463)
Standard curve (colourimetric) : mean of duplicates (+/-SD) with background readings substracted
Functional Studies - Thioredoxin Reductase Assay Kit (Colorimetric) (ab83463)
Thioredoxin reductase Kinetic Data using ab83463.
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