Thioredoxin Reductase Assay Kit (Colorimetric)

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Supplier Data

Functional Studies - Thioredoxin Reductase Assay Kit (Colorimetric) (AB83463)

Thioredoxin reductase Kinetic Data using ab83463.

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Lab

Functional Studies - Thioredoxin Reductase Assay Kit (Colorimetric) (AB83463)

Standard curve (colourimetric) : mean of duplicates (+/-SD) with background readings substracted

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Lab

Functional Studies - Thioredoxin Reductase Assay Kit (Colorimetric) (AB83463)

Thioredoxin reductase measured in cell lysates showing activity (mU) per 1 mln of cells tested

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Lab

Functional Studies - Thioredoxin Reductase Assay Kit (Colorimetric) (AB83463)

Thioredoxin reductase measured in mouse tissue lysates showing activity (mU) per mg protein of sample tested

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PubMed

Functional Studies - Thioredoxin Reductase Assay Kit (Colorimetric) (AB83463)

Activity of endogenous Brugia thioredoxin reductase from soluble worm lysates following incubation with 1% DMSO or 0.3 μM, 0.1 μM, or 0.03 μM of auranofin in vitro. Percentages indicate the percent activity of TrxR compared to DMSO controls. Thioredoxin reductase activity was significantly reduced (p < 0.05) to 15%, 33% and 69% of endogenous activity, respectively, compared to the activity in DMSO-treated worms.

Thioredoxin reductase activity of worm lysates was assayed using female B. malayi treated in vitro with either 0.3 μM, 0.1 μM, or 0.03 μM auranofin or 1% DMSO. After 5 hours of treatment, worm motility was measured using the Worminator, and then worms (24 in each group) were pooled, washed three times in PBS, and lysed by douncing in a glass homogenizer in assay buffer (ab83463) with 1 mM PMSF. The crude lysates were centrifuged at 10,000 rcf for 15 minutes at 4°C to pellet insoluble material. The total protein concentrations of soluble lysates were measured using the Bradford assay. The soluble lysates were incubated for 20 minutes in assay buffer or assay buffer with a proprietary thioredoxin reductase specific inhibitor before adding a specific substrate, DTNB (5, 5′-dithiobis (2-nitrobenzoic) acid), and measuring activity at 20 second intervals for 40 minutes using the SpectraMax Plus Microplate Reader (Molecular Devices, Sunnyvale, CA) at λ = 412 nm. Lysates were tested in duplicate. TrxR activity was calculated based on the linear amount of TNB produced per minute per mg of total protein and adjusted for background activity from enzymes other than TrxR in the lysates.

Image from Bulman CA et al., PLoS One. 2015;9(2):e0003534. Fig 4(A).; doi: 10.1371/journal.pntd.0003534. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

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Supplier Data

Other - Thioredoxin Reductase Assay Kit (Colorimetric) (AB83463)

Representative image of Thioredoxin Reductase Assay Kit (Colorimetric) ab83463

Components shown from left to right :

- DTNB

- TNB Standard

- NADPH I

- Thioredoxin Reductase Positive Control

- Thioredoxin Reductase Inhibitor

- Thioredoxin Reductase Assay Buffer

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit.