- Biochemical assay
Supplier Data
Biochemical assay - Threonine Assay Kit (AB239726)
Estimation of total L-Threonine in pooled normal human plasma (10 μl), single donor off-the-clot human serum (5 μl) and pooled human CSF (10 μl).
L-Threonine concentrations for plasma, serum and CSF samples were 93.26 ± 3.72 μM, 159.7 ± 8.36 μM and 70.92 ± 3.12 μM, respectively. Data are mean ± SEM of at least 3 replicates, samples were deproteinized using 10 kDa MWCO spin columns and assayed according to the kit protocol.
- Biochemical assay
Supplier Data
Biochemical assay - Threonine Assay Kit (AB239726)
Specificity for detection of L-Threonine (L-THR) over D-Threonine and other common amino acids.
At a 10-fold molar excess (5 nmole/well) versus L-Threonine (500 pmole/well), all other amino acids tested contribute ≤5% interference.
- Biochemical assay
Supplier Data
Biochemical assay - Threonine Assay Kit (AB239726)
Threonine Standard Curve
Product details
The Threonine Assay Kit (ab239726) allows for highly sensitive quantification of L-Threonine levels in biological fluids and tissues. The assay is based on the selective, NAD+-coupled enzymatic metabolism of threonine, yielding an oxidized intermediate and NADH. A developer enzyme mixture utilizes the NADH generated to convert the probe into a stable fluorophore (Ex/Em = 535/587 nm). The assay is not affected by physiological concentrations of other amino acids, is high-throughput adaptable and can detect threonine levels down to 2 μM in samples.
Sample Type:
- Human or animal biological fluids (plasma, serum, CSF, etc.)
- Soft tissue homogenates (i.e. liver, brain, etc.)
- Cultured cell lysates (adherent or suspension cells) or cell culture growth medium
This product is manufactured by BioVision, an Abcam company and was previously called K463 PicoProbe™ Threonine Assay Kit (Fluorometric). K463-100 is the same size as the 100 test size of ab239726.
The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.
What's included?
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
This amino acid plays several roles beyond protein synthesis. Threonine acts as a precursor for glycine and serine in metabolic processes and participates in the immune function by supporting the production of antibodies. Its involvement in the synthesis of mucin proteins highlights its importance in gut health. Threonine is also part of the complex that involves tissue development and acts synergistically with other proteins in maintaining the balance of neural networks.
Pathways
Threonine is integral to cellular metabolism particularly within the threonine degradation pathway that leads to acetyl-CoA production. This pathways intersections with the citric acid cycle a central metabolic hub. Furthermore threonine is also connected to the mTOR signaling pathway which regulates cell growth and proliferation. Proteins like serine and methionine have a close metabolic relationship with threonine sharing metabolic intermediates and pathways.
Product protocols
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Target data
Publications (2)
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Nature communications 13:6554 PubMed36323683
2022
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The British journal of dermatology 186:532-543 PubMed34545566
2021
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