Thrombin Activity Assay Kit (Colorimetric)
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(4 Publications)
Thrombin Activity Assay Kit ab234620 is developed to determine thrombin activity in human plasma, serum, tissue, cell lysates and cell culture supernatant samples. Readout on any colorimetric (405 nm) plate reader.
View Alternative Names
Prothrombin, Coagulation factor II, F2
- FuncS
Supplier Data
Functional Studies - Thrombin Activity Assay Kit (Colorimetric) (AB234620)
Human Thrombin Chromogenic Activity Standard Curve after 2 hours.
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Product details
Thrombin Activity Assay Kit (Colorimetric) (ab234620) is developed to determine thrombin activity in human plasma, serum, tissue, cell lysates and cell culture samples. The amidolytic activity of thrombin is quantitated using a highly specific thrombin substrate releasing a pNA chromophore. The change in absorbance of the pNA at 405 nm is directly proportional to the thrombin enzymatic activity.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
Other Notes
Thrombin (activated factor II [IIa]) is a coagulation protein that has many effects in the coagulation cascade. Thrombin is a serine protease (EC 3.4.21.5) that converts soluble fibrinogen into insoluble strands of fibrin, as well as catalyzing many other coagulation-related reactions. Thrombin is in the form of alpha-thrombin that is the immediate end product of prothrombin activation: two further thrombin products can be identified, beta- and gamma- thrombin. These are degraded forms that may arise from autodigestion of a thrombin preparation.
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Supplementary information
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Biological function summary
Thrombin modulates several physiological processes beyond clot formation. It serves as a signaling molecule interacting with protease-activated receptors (PARs) to influence cell functions including proliferation migration and apoptosis. Thrombin forms part of the prothrombinase complex comprised of prothrombin activated Factor X (Xa) and Factor V on phospholipid surfaces. This complex is critical for thrombin generation during the clotting cascade. Thrombin's activity extends to involvement in wound healing and inflammation regulation.
Pathways
Thrombin significantly participates in the coagulation and fibrinolytic pathways. It not only converts fibrinogen to fibrin in the coagulation pathway but also activates inhibitors like antithrombin which regulates thrombin and other protease activities. Thrombin's interaction with fibrinolysis where tPA and plasminogen are substrates integrates clot disintegration processes. Thrombin's connectivity with proteins such as Factor VII Protein C and antithrombin outlines its diverse role in maintaining hemostatic balance.
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Publications (4)
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Cell communication and signaling : CCS 23:210 PubMed40312345
2025
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Anticancer research 44:3269-3276 PubMed39060075
2024
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Frontiers in pharmacology 14:1304697 PubMed38143504
2023
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Scientific reports 11:21852 PubMed34750441
2021
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