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AB197006

Thrombin Activity Assay Kit (Fluorometric)

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(7 Publications)

Thrombin Activity Assay Kit (Fluorometric) (ab197006) is simple, rapid and can detect Thrombin activity as low as 1 ng in samples. Readout on any fluorometric (Ex/Em 350/450 nm) plate reader.

Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.

View Alternative Names

Prothrombin, Coagulation factor II, F2

2 Images
Functional Studies - Thrombin Activity Assay Kit (Fluorometric) (AB197006)
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Supplier Data

Functional Studies - Thrombin Activity Assay Kit (Fluorometric) (AB197006)

Thrombin Activity in Plasma.

Thrombin activity was measured in plasma samples in the presence and absence of aThrombin inhibitor, PPACK Dihydrochloride. S = Substrate, I = Inhibitor, AB = Activation Buffer containing Factor Xa. Assays were performed following the kit protocol.

Functional Studies - Thrombin Activity Assay Kit (Fluorometric) (AB197006)
  • FuncS

Supplier Data

Functional Studies - Thrombin Activity Assay Kit (Fluorometric) (AB197006)

Standard plot of Thrombin Activity

Key facts

Detection method

Fluorescent

Sample types

Purified protein, Plasma

Results type

Quantitative

Sensitivity

= 1 ng

Assay Platform

Microplate reader

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Primary Antibodies

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Enzyme activity assay": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Thrombin Activity Assay Kit (Fluorometric) (ab197006) is simple, rapid and can detect Thrombin activity as low as 1 ng in samples.

In the thrombin activity assay protocol, a synthetic AMC-based peptide substrate is proteolytically cleaved by thrombin to release a fluorophore, AMC, which can be easily quantified by fluorescence reader at Ex/Em = 350/450 nm.

Thrombin activity assay protocol summary:
- add standards and samples to wells
- add reaction mix
- analyze with a microplate reader for 30-60 min

Other Notes
This product is manufactured by BioVision, an Abcam company and was previously called K373 Thrombin Activity Fluorometric Assay Kit. K373-100 is the same size as the 100 test size of ab197006.

Thrombin enzyme (Factor IIa, EC 3.4.21.5), a serine protease, is an important clotting factor in the coagulation cascade that involves the conversion of soluble fibrinogen to insoluble active fibrin strands.

The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.

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What's included?

{ "values": { "100Test": { "sellingSize": "100 Test", "publicAssetCode":"ab197006-100Test", "assetComponentDetails": [ { "size":"1 x 0.5 mL", "name":"Thrombin Substrate", "number":"AB197006-CMP04", "productcode":"" }, { "size":"1 x 1 mL", "name":"Thrombin Dilution Buffer", "number":"AB197006-CMP02", "productcode":"" }, { "size":"1 x 15 mL", "name":"Thrombin Assay Buffer", "number":"AB197006-CMP01", "productcode":"" }, { "size":"1 x 5 µL", "name":"Thrombin Positive control", "number":"AB197006-CMP03", "productcode":"" }, { "size":"1 x 100 µL", "name":"AMC Standard", "number":"AB197006-CMP05", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Thrombin also known as Factor II or thrombin protein is a 36 kDa serine protease essential for blood coagulation. It is produced in the liver as prothrombin and activated in the coagulation cascade. Its expression occurs extensively in the liver where it plays an important role in converting soluble fibrinogen into insoluble fibrin forming blood clots. Thrombin also activates various other coagulation factors including Factors V VIII and XI amplifying the coagulation response. Biotinylated thrombin variations help in detection and research applications.
Biological function summary

Thrombin modulates several physiological processes beyond clot formation. It serves as a signaling molecule interacting with protease-activated receptors (PARs) to influence cell functions including proliferation migration and apoptosis. Thrombin forms part of the prothrombinase complex comprised of prothrombin activated Factor X (Xa) and Factor V on phospholipid surfaces. This complex is critical for thrombin generation during the clotting cascade. Thrombin's activity extends to involvement in wound healing and inflammation regulation.

Pathways

Thrombin significantly participates in the coagulation and fibrinolytic pathways. It not only converts fibrinogen to fibrin in the coagulation pathway but also activates inhibitors like antithrombin which regulates thrombin and other protease activities. Thrombin's interaction with fibrinolysis where tPA and plasminogen are substrates integrates clot disintegration processes. Thrombin's connectivity with proteins such as Factor VII Protein C and antithrombin outlines its diverse role in maintaining hemostatic balance.

Thrombin associates with conditions such as venous thromboembolism and disseminated intravascular coagulation (DIC). Dysregulation of thrombin leads to excessive clotting contributing to thromboembolic diseases. In DIC uncontrolled thrombin activity results in systemic coagulation consuming clotting factors and increasing bleeding risk. Its function alongside proteins like Factor V Leiden and antithrombin deficiencies aggravate thrombotic conditions. Research into thrombin inhibitors aids in developing therapeutic strategies for these disorders.
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Product protocols

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Target data

Thrombin, which cleaves bonds after Arg and Lys, converts fibrinogen to fibrin and activates factors V, VII, VIII, XIII, and, in complex with thrombomodulin, protein C. Functions in blood homeostasis, inflammation and wound healing. Activates coagulation factor XI (F11); activation is promoted by the contact with negatively charged surfaces (PubMed : 2019570, PubMed : 21976677). Triggers the production of pro-inflammatory cytokines, such as MCP-1/CCL2 and IL8/CXCL8, in endothelial cells (PubMed : 30568593, PubMed : 9780208).
See full target information F2
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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Polymers 15: PubMed37447564

2023

Injectable Lyophilized Chitosan-Thrombin-Platelet-Rich Plasma (CS-FIIa-PRP) Implant to Promote Tissue Regeneration: In Vitro and Ex Vivo Solidification Properties.

Applications

Unspecified application

Species

Unspecified reactive species

Fiona Milano,Anik Chevrier,Gregory De Crescenzo,Marc Lavertu

Experimental animals 71:305-315 PubMed35173110

2022

MicroRNA-363-3p/sphingosine-1-phosphate receptor 1 axis inhibits sepsis-induced acute lung injury via the inactivation of nuclear factor kappa-B ligand signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Shishuai Meng,Kai Kang,Dongsheng Fei,Songlin Yang,Shangha Pan,Kaijiang Yu,Mingyan Zhao

International journal of molecular sciences 22: PubMed33923802

2021

Antiplatelet Therapy Combined with Anastrozole Induces Features of Partial EMT in Breast Cancer Cells and Fails to Mitigate Breast-Cancer Induced Hypercoagulation.

Applications

Unspecified application

Species

Unspecified reactive species

Kutlwano R Xulu,Tanya N Augustine

Scientific reports 10:19256 PubMed33159119

2020

Tamoxifen induces hypercoagulation and alterations in ERα and ERβ dependent on breast cancer sub-phenotype ex vivo.

Applications

Unspecified application

Species

Unspecified reactive species

K Pather,T N Augustine

Proceedings of the National Academy of Sciences of 117:10958-10969 PubMed32366656

2020

Targeted inhibition of thrombin attenuates murine neonatal necrotizing enterocolitis.

Applications

Unspecified application

Species

Unspecified reactive species

Kopperuncholan Namachivayam,Krishnan MohanKumar,Darla R Shores,Sunil K Jain,Jennifer Fundora,Allen D Everett,Ling He,Hua Pan,Samuel A Wickline,Akhil Maheshwari

International journal of hematology 110:59-68 PubMed31006077

2019

Timely and large dose of clotting factor IX provides better joint wound healing after hemarthrosis in hemophilia B mice.

Applications

Unspecified application

Species

Unspecified reactive species

Ping He,Feixu Zhang,Chen Zhong,Min Li,Jing Zheng,Baolai Hua,Junjiang Sun

Carbohydrate polymers 208:168-179 PubMed30658788

2019

Enhancement of hemostatic property of plant derived oxidized nanocellulose-silk fibroin based scaffolds by thrombin loading.

Applications

Unspecified application

Species

Unspecified reactive species

Anha Afrin Shefa,Mirana Taz,Sun Young Lee,Byong-Taek Lee
View all publications
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Product promise

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