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AB316246

Tissue Clearing Kit – CUBIC

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(6 Publications)

Tissue Clearing Kit – CUBIC (ab316246) enables the easy set-up of rapid tissue clearing for 3D tissue imaging using simple protocols and standard laboratory equipment.
4 Images
IHC-tissue clearing - Tissue Clearing Kit – CUBIC (AB316246)
  • IHC-tissue clearing

Supplier Data

IHC-tissue clearing - Tissue Clearing Kit – CUBIC (AB316246)

Anti-Iba1 ab178846 was used with Tissue clearing kit – CUBIC (ab316246) and 3D Tissue Staining Kit – CUBIC (ab316248) to penetrate stain and clear a whole mouse brain.

Learn more about tissue clearing kits reagents and protocols designed to make it easier to stain whole brains and get more data from each valuable tissue sample.

For a whole mouse brain we recommend starting with 3.5 ug of ab178846 and using a Fab fragment secondary antibody with 2.34 µg to create an antibody complex before 3D staining (see protocol for details). Additive A was used during the staining process.

The sample was imaged using a light-sheet microscope.

IHC-tissue clearing - Tissue Clearing Kit – CUBIC (AB316246)
  • IHC-tissue clearing

Supplier Data

IHC-tissue clearing - Tissue Clearing Kit – CUBIC (AB316246)

Anti-NeuN ab177487 was used with Tissue clearing kit – CUBIC (ab316246) and 3D Tissue Staining Kit – CUBIC (ab316248) to penetrate stain and clear a whole mouse brain.

White : nuclear staining
Red : NeuN.

Learn more about tissue clearing kits reagents and protocols designed to make it easier to stain whole brains and get more data from each valuable tissue sample. For a whole mouse brain we recommend starting with 10 ug of ab177487 and using a Fab fragment secondary antibody with 6.67 µg to create an antibody complex before 3D staining (see protocol for details). Additive A was used during the staining process.

The sample was imaged using a light-sheet microscope.

IHC-tissue clearing - Tissue Clearing Kit – CUBIC (AB316246)
  • IHC-tissue clearing

Supplier Data

IHC-tissue clearing - Tissue Clearing Kit – CUBIC (AB316246)

After clearing the whole mouse brain with the Tissue clearing kit – CUBIC (ab316246) anti-c-FOS ab222699 was used with the 3D Tissue Staining Kit – CUBIC (ab316248). White : nuclear staining Yellow : c-FOS. The sample was imaged using a light-sheet microscope.

IHC-tissue clearing - Tissue Clearing Kit – CUBIC (AB316246)
  • IHC-tissue clearing

Supplier Data

IHC-tissue clearing - Tissue Clearing Kit – CUBIC (AB316246)

After clearing the whole mouse brain with the Tissue clearing kit – CUBIC (ab316246) anti-CD31 ab213175 was used with the 3D Tissue Staining Kit – CUBIC (ab316248). The color in the image represents the depth of the signal. The sample was imaged using a light-sheet microscope .

Key facts

Reacts with

Mouse

Assay platform

Reagents

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "IHC-tissue clearing": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Tissue Clearing Kit – CUBIC (ab316246) enables the easy set-up of rapid tissue clearing for 3D tissue imaging using simple protocols and standard laboratory equipment.

Together with the 3D Tissue Staining Kit – CUBIC (ab316248) we provide a versatile tissue clearing and 3D tissue staining method that allows the observation of all cells in organs and the body. The kits are designed for safe and easy use in the lab.

  • Comes with water-soluble clearing reagents that achieve transparency of organs and the whole body
  • Can be performed with only general lab equipment and does not require special dedicated equipment such as an electrophoresis apparatus.
  • Uses light-sheet microscopes (LSFM) and confocal laser scanning microscopes to allow imaging of the whole 3D organ with a resolution to the cellular level.

The provided protocol solves issues pertaining to safety, waste processing, microscope compatibility, fluorescent protein signal retainability, etc.

To learn more about tissue clearing, and tissue clearing methods (including for 3D cell cultures), please review our guide to tissue clearing.

These tissue clearing reagents are using CUBIC technology.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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What's included?

{ "values": { "20Tests": { "sellingSize": "20 Tests", "publicAssetCode":"ab316246-20Tests", "assetComponentDetails": [ { "size":"1 x 500 mL", "name":"CUBIC-R", "number":"AB316246-CMP02" }, { "size":"1 x 500 mL", "name":"CUBIC-L", "number":"AB316246-CMP01" } ] }, "10Tests": { "sellingSize": "10 Tests", "publicAssetCode":"ab316246-10Tests", "assetComponentDetails": [ { "size":"1 x 250 mL", "name":"CUBIC-R", "number":"AB316246-CMP02", "productcode":"" }, { "size":"1 x 250 mL", "name":"CUBIC-L", "number":"AB316246-CMP01", "productcode":"" } ] } } }
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Properties and storage information

Shipped at Conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
Ambient
Appropriate long-term storage conditions
Ambient
Storage information
Ambient
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Nature protocols 14:3506-3537 PubMed31748753

2019

Advanced CUBIC tissue clearing for whole-organ cell profiling.

Applications

IHC-tissue clearing

Species

Unspecified reactive species

Katsuhiko Matsumoto,Tomoki T Mitani,Shuhei A Horiguchi,Junichi Kaneshiro,Tatsuya C Murakami,Tomoyuki Mano,Hiroshi Fujishima,Ayumu Konno,Tomonobu M Watanabe,Hirokazu Hirai,Hiroki R Ueda

Cell reports 24:2196-2210.e9 PubMed30134179

2018

Chemical Landscape for Tissue Clearing Based on Hydrophilic Reagents.

Applications

IHC-tissue clearing

Species

Unspecified reactive species

Kazuki Tainaka,Tatsuya C Murakami,Etsuo A Susaki,Chika Shimizu,Rie Saito,Kei Takahashi,Akiko Hayashi-Takagi,Hiroshi Sekiya,Yasunobu Arima,Satoshi Nojima,Masako Ikemura,Tetsuo Ushiku,Yoshihiro Shimizu,Masaaki Murakami,Kenji F Tanaka,Masamitsu Iino,Haruo Kasai,Toshikuni Sasaoka,Kazuto Kobayashi,Kohei Miyazono,Eiichi Morii,Tadashi Isa,Masashi Fukayama,Akiyoshi Kakita,Hiroki R Ueda

Scientific reports 7:9269 PubMed28839164

2017

CUBIC pathology: three-dimensional imaging for pathological diagnosis.

Applications

IHC-tissue clearing

Species

Unspecified reactive species

Satoshi Nojima,Etsuo A Susaki,Kyotaro Yoshida,Hiroyoshi Takemoto,Naoto Tsujimura,Shohei Iijima,Ko Takachi,Yujiro Nakahara,Shinichiro Tahara,Kenji Ohshima,Masako Kurashige,Yumiko Hori,Naoki Wada,Jun-Ichiro Ikeda,Atsushi Kumanogoh,Eiichi Morii,Hiroki R Ueda

Cell reports 20:236-250 PubMed28683317

2017

Whole-Body Profiling of Cancer Metastasis with Single-Cell Resolution.

Applications

IHC-tissue clearing

Species

Unspecified reactive species

Shimpei I Kubota,Kei Takahashi,Jun Nishida,Yasuyuki Morishita,Shogo Ehata,Kazuki Tainaka,Kohei Miyazono,Hiroki R Ueda

Cell 159:911-24 PubMed25417165

2014

Whole-body imaging with single-cell resolution by tissue decolorization.

Applications

IHC-tissue clearing

Species

Unspecified reactive species

Kazuki Tainaka,Shimpei I Kubota,Takeru Q Suyama,Etsuo A Susaki,Dimitri Perrin,Maki Ukai-Tadenuma,Hideki Ukai,Hiroki R Ueda

Cell 157:726-39 PubMed24746791

2014

Whole-brain imaging with single-cell resolution using chemical cocktails and computational analysis.

Applications

IHC-tissue clearing

Species

Unspecified reactive species

Etsuo A Susaki,Kazuki Tainaka,Dimitri Perrin,Fumiaki Kishino,Takehiro Tawara,Tomonobu M Watanabe,Chihiro Yokoyama,Hirotaka Onoe,Megumi Eguchi,Shun Yamaguchi,Takaya Abe,Hiroshi Kiyonari,Yoshihiro Shimizu,Atsushi Miyawaki,Hideo Yokota,Hiroki R Ueda
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com