Tissue type Plasminogen Activator Activity Assay Kit (Colorimetric, Human)

Product details

Tissue type Plasminogen Activator Activity Assay Kit (Colorimetric, Human) ab108905 is used to measure Human Tissue type Plasminogen Activator activity in serum and plasma samples.

The tPA assay protocol measures the ability of Tissue type Plasminogen Activator to activate the plasminogen to plasmin in coupled or indirect assays that contain Tissue type Plasminogen Activator, plasminogen, and a plasmin-specific synthetic substrate. The amount of plasmin produced is quantitated using a highly specific plasmin substrate releasing a yellow para-nitroaniline (pNA) chromophore. The change in absorbance of the pNA in the reaction solution at 405 nm is directly proportional to the Tissue type Plasminogen Activator enzymatic activity.

The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

Previously called Tissue type Plasminogen Activator Human Chromogenic Activity Assay Kit.

Tissue type Plasminogen Activator (tPA) is a 68 kDa serine protease that converts the zymogen plasminogen into the active serine protease plasmin which digests fibrin and induces the dissolution of fibrin clots. Tissue type Plasminogen Activator is synthesized by endothelial cells in normal blood vessels and displays relatively high affinity for fibrin, suggesting that it functions predominately in physiological thrombolysis in vivo.