AB228569

TMRM Assay Kit (Mitochondrial Membrane Potential)

Name: TMRM Assay Kit (Mitochondrial Membrane Potential) (ab228569) | Abcam
Brand: Abcam Limited
SKU: AB228569
Price: 370 USD
Availability: InStock
Rating: 5 (1 reviews)

(1 Review)

(18 Publications)

TMRM Assay Kit (Mitochondrial Membrane Potential) ab228569 is a complete kit for TMRM staining of cell cultures. Readout by flow cytometry, fluorescence plate reader, or fluorescence microscopy (Ex/Em 548/573 nm). Contains TMRM reagent, buffer and control.

1 Images

Immunocytochemistry/ Immunofluorescence - TMRM Assay Kit (Mitochondrial Membrane Potential) (AB228569)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - TMRM Assay Kit (Mitochondrial Membrane Potential) (AB228569)

Human fibroblast cells stained using ab228569 TMRM Assay Kit (Mitochondrial Membrane Potential) (200 nM TMRM).

Key facts

Detection method

Fluorescent

Sample types

Suspension cells, Adherent cells

Assay type

Cell-based

Assay Platform

Microplate reader, Fluor. microscope, Flow cyt.

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Product details

TMRM Assay Kit (Mitochondrial Membrane Potential) ab228569 is designed to quantify changes in mitochondrial membrane potential in living cells using flow cytometry, fluorescence plate reader, or fluorescence microscopy. It uses the red-fluorescent probe TMRM (tetramethylrhodamine, methyl ester) that localizes in mitochondria and detects mitochondrial membrane depolarization.

Related assay kits
TMRE is the ethyl ester of tetramethylrhodamine and is a close equivalent of TMRM. TMRE is used about 3 times more than TMRM for mitochondrial membrane potential assays.
TMRE-Mitochondrial Membrane Potential Assay Kit ab113852 is our most popular assay kit for mitochondrial membrane potential analysis.

TMRM staining is used to monitor mitochondrial function. When accumulated in a negatively charge polarized mitochondria, TMRM fluoresces orange. When mitochondrial membrane potential collapses in apoptotic or metabolically stressed cells, the TMRM reagent is dispersed throughout the cell cytosol and fluorescence levels drop dramatically. This kit provides enough reagents to analyze 5 x 96 wells (500 samples).

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What's included?

{ "values": { "1Kit": { "sellingSize": "1 Kit", "publicAssetCode":"ab228569-1Kit", "assetComponentDetails": [ { "size":"1 x 25 µL", "name":"FCCP", "number":"AB228569-CMP02", "productcode":"" }, { "size":"1 x 30 mL", "name":"5X Live Cell Imaging Buffer", "number":"AB228569-CMP01", "productcode":"" }, { "size":"1 x 100 µL", "name":"TMRM Reagent", "number":"AB228569-CMP03", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

Multi

Appropriate long-term storage conditions

Multi

Storage information

Please refer to protocols

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Product protocols

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Target data

See full target information Tetramethylrhodamine methyl ester perchlorate

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Publications (18)

Recent publications for all applications. Explore the full list and refine your search

Naunyn-Schmiedeberg's archives of pharmacology 398:12745-12756 PubMed40214725

2025

A sesquiterpene lactone, tomentosin, as a novel anticancer agent: orchestrating apoptosis, autophagy, and ER stress in colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Sümeyra Çetinkaya,Ebru Güçlü,İlknur Çınar Ayan,Hasibe Vural,Hatice Gül Dursun

eLife 12: PubMed39625057

2024

An acute microglial metabolic response controls metabolism and improves memory.

Applications

Unspecified application

Species

Unspecified reactive species

Anne Drougard,Eric H Ma,Vanessa Wegert,Ryan Sheldon,Ilaria Panzeri,Naman Vatsa,Stefanos Apostle,Luca Fagnocchi,Judith Schaf,Klaus Gossens,Josephine Völker,Shengru Pang,Anna Bremser,Erez Dror,Francesca Giacona,Sagar Sagar,Michael X Henderson,Marco Prinz,Russell G Jones,John Andrew Pospisilik

Experimental & molecular medicine 56:2673-2689 PubMed39623092

2024

EPRS1-mediated fibroblast activation and mitochondrial dysfunction promote kidney fibrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Seung Seob Son,Hee Seul Jeong,Seong-Woo Lee,Eun Soo Lee,Jeong Geon Lee,Ji-Hye Lee,Jawoon Yi,Mi Ju Park,Min Sun Choi,Donghyeong Lee,Sin Young Choi,Jiheon Ha,Jeong Suk Kang,Nam-Jun Cho,Samel Park,Hyo-Wook Gil,Choon Hee Chung,Joon Seok Park,Myung Hee Kim,Jihwan Park,Eun Young Lee

The EMBO journal 43:4870-4891 PubMed39284909

2024

IF1 is a cold-regulated switch of ATP synthase hydrolytic activity to support thermogenesis in brown fat.

Applications

Unspecified application

Species

Unspecified reactive species

Henver S Brunetta,Anna S Jung,Fernando Valdivieso-Rivera,Stepheny C de Campos Zani,Joel Guerra,Vanessa O Furino,Annelise Francisco,Marcelo Berçot,Pedro M Moraes-Vieira,Susanne Keipert,Martin Jastroch,Laurent O Martinez,Carlos H Sponton,Roger F Castilho,Marcelo A Mori,Alexander Bartelt

Cells 13: PubMed39120284

2024

Dysfunction of Mitochondrial Dynamics Induces Endocytosis Defect and Cell Damage in Nephrocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Jun-Yi Zhu,Jianli Duan,Joyce van de Leemput,Zhe Han

International journal of molecular sciences 25: PubMed39000233

2024

TXNDC5 Plays a Crucial Role in Regulating Endoplasmic Reticulum Activity through Different ER Stress Signaling Pathways in Hepatic Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Seyed Hesamoddin Bidooki,Cristina Barranquero,Javier Sánchez-Marco,Roberto Martínez-Beamonte,María J Rodríguez-Yoldi,María A Navarro,Susana C M Fernandes,Jesús Osada

The Journal of clinical investigation 134: PubMed38015641

2024

RAB7 deficiency impairs pulmonary artery endothelial function and promotes pulmonary hypertension.

Applications

Unspecified application

Species

Unspecified reactive species

Bryce Piper,Srimathi Bogamuwa,Tanvir Hossain,Daniela Farkas,Lorena Rosas,Adam C Green,Geoffrey Newcomb,Nuo Sun,Jose A Ovando-Ricardez,Jeffrey C Horowitz,Aneel R Bhagwani,Hu Yang,Tatiana V Kudryashova,Mauricio Rojas,Ana L Mora,Pearlly Yan,Rama K Mallampalli,Elena A Goncharova,David M Eckmann,Laszlo Farkas

Antioxidants (Basel, Switzerland) 12: PubMed38136224

2023

Mitochondrial Stress Links Environmental Triggers with Pro-Inflammatory Signaling in Crohn's Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Flores Martín-Reyes,Manuel Bernal,Cristina Rodríguez-Díaz,Damaris Rodríguez-de Los Reyes,Ailec Ho-Plagaro,Francisca Rodríguez-Pacheco,Laura Camacho-Martel,Raquel Camargo-Camero,Francisco J Rodríguez-González,Guillermo Alcain-Martínez,Rafael Martín-Masot,Víctor M Navas-López,Marina Villanueva-Paz,María Isabel Lucena,Eduardo García-Fuentes,Carlos López-Gómez

Methods in molecular biology (Clifton, N.J.) 2712:103-115 PubMed37578700

2023

Monitoring Mitochondria Function in Ferroptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Fangquan Chen,Jiao Liu,Daolin Tang,Rui Kang

Cells 12: PubMed37174736

2023

The Pesticide Chlordecone Promotes Parkinsonism-like Neurodegeneration with Tau Lesions in Midbrain Cultures and Worms.

Applications

Unspecified application

Species

Unspecified reactive species

Valeria Parrales-Macias,Patrick P Michel,Aurore Tourville,Rita Raisman-Vozari,Stéphane Haïk,Stéphane Hunot,Nicolas Bizat,Annie Lannuzel

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

TMRM Assay Kit (Mitochondrial Membrane Potential)

Immunocytochemistry/ Immunofluorescence - TMRM Assay Kit (Mitochondrial Membrane Potential) (AB228569)

Key facts

Detection method

Sample types

Assay type

Assay Platform

Product details

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (18)

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