TMRM Assay Kit (Mitochondrial Membrane Potential) ab228569 is a complete kit for TMRM staining of cell cultures. Readout by flow cytometry, fluorescence plate reader, or fluorescence microscopy (Ex/Em 548/573 nm). Contains TMRM reagent, buffer and control.
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- The Journal of clinical investigation 134:2024RAB7 deficiency impairs pulmonary artery endothelial function and promotes pulmonary hypertension.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBryce Piper,Srimathi Bogamuwa,Tanvir Hossain,Daniela Farkas,Lorena Rosas,Adam C Green,Geoffrey Newcomb,Nuo Sun,Jose A Ovando-Ricardez,Jeffrey C Horowitz,Aneel R Bhagwani,Hu Yang,Tatiana V Kudryashova,Mauricio Rojas,Ana L Mora,Pearlly Yan,Rama K Mallampalli,Elena A Goncharova,David M Eckmann,Laszlo FarkasPubMed 38015641
- Antioxidants (Basel, Switzerland) 12:2023Mitochondrial Stress Links Environmental Triggers with Pro-Inflammatory Signaling in Crohn's Disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFlores Martín-Reyes,Manuel Bernal,Cristina Rodríguez-Díaz,Damaris Rodríguez-de Los Reyes,Ailec Ho-Plagaro,Francisca Rodríguez-Pacheco,Laura Camacho-Martel,Raquel Camargo-Camero,Francisco J Rodríguez-González,Guillermo Alcain-Martínez,Rafael Martín-Masot,Víctor M Navas-López,Marina Villanueva-Paz,María Isabel Lucena,Eduardo García-Fuentes,Carlos López-GómezPubMed 38136224
- Methods in molecular biology (Clifton, N.J.) 2712:103-1152023Monitoring Mitochondria Function in Ferroptosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFangquan Chen,Jiao Liu,Daolin Tang,Rui KangPubMed 37578700
- Cells 12:2023The Pesticide Chlordecone Promotes Parkinsonism-like Neurodegeneration with Tau Lesions in Midbrain Cultures and Worms.Applications:Unspecified applicationReactive species:Unspecified reactive speciesValeria Parrales-Macias,Patrick P Michel,Aurore Tourville,Rita Raisman-Vozari,Stéphane Haïk,Stéphane Hunot,Nicolas Bizat,Annie LannuzelPubMed 37174736
- Antioxidants (Basel, Switzerland) 12:2023Membrane Vesicles of Toxigenic Affect the Metabolism of Liver HepG2 Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEstefanía Caballano-Infantes,Ailec Ho-Plágaro,Carlos López-Gómez,Flores Martín-Reyes,Francisca Rodríguez-Pacheco,Bernard Taminiau,Georges Daube,Lourdes Garrido-Sánchez,Guillermo Alcaín-Martínez,Raúl J Andrade,Miren García-Cortés,M Isabel Lucena,Eduardo García-Fuentes,Cristina Rodríguez-DíazPubMed 37107193
- Fluids and barriers of the CNS 20:192023Morphological and mitochondrial changes in murine choroid plexus epithelial cells during healthy aging.Applications:Unspecified applicationReactive species:Unspecified reactive speciesValentina Scarpetta,Felipe Bodaleo,Chiara Salio,Amit Agarwal,Marco Sassoè-Pognetto,Annarita PatriziPubMed 36918889
- Nature communications 14:8712023A trans-kingdom T6SS effector induces the fragmentation of the mitochondrial network and activates innate immune receptor NLRX1 to promote infection.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJoana Sá-Pessoa,Sara López-Montesino,Kornelia Przybyszewska,Isabel Rodríguez-Escudero,Helina Marshall,Adelia Ova,Gunnar N Schroeder,Peter Barabas,María Molina,Tim Curtis,Víctor J Cid,José A BengoecheaPubMed 36797302
- Cell death & disease 14:612023Inhibition of autophagy and MEK promotes ferroptosis in Lkb1-deficient Kras-driven lung tumors.Applications:Unspecified applicationReactive species:Unspecified reactive speciesVrushank Bhatt,Taijin Lan,Wenping Wang,Jerry Kong,Eduardo Cararo Lopes,Jianming Wang,Khoosheh Khayati,Akash Raju,Michael Rangel,Enrique Lopez,Zhixian Sherrie Hu,Xuefei Luo,Xiaoyang Su,Jyoti Malhotra,Wenwei Hu,Sharon R Pine,Eileen White,Jessie Yanxiang GuoPubMed 36702816
- Frontiers in immunology 12:7998962022Mitochondrial Dysfunction Associates With Acute T Lymphocytopenia and Impaired Functionality in COVID-19 Patients.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYufei Mo,Kelvin Kai-Wang To,Runhong Zhou,Li Liu,Tianyu Cao,Haode Huang,Zhenglong Du,Chun Yu Hubert Lim,Lok-Yan Yim,Tsz-Yat Luk,Jacky Man-Chun Chan,Thomas Shiu-Hong Chik,Daphne Pui-Ling Lau,Owen Tak-Yin Tsang,Anthony Raymond Tam,Ivan Fan-Ngai Hung,Kwok-Yung Yuen,Zhiwei ChenPubMed 35095881
- Immunology 165:61-732021IL-33 activates mTORC1 and modulates glycolytic metabolism in CD8 T cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYuejin Liang,Xiaofang Wang,Hui Wang,Wenjing Yang,Panpan Yi,Lynn Soong,Yingzi Cong,Jiyang Cai,Xuegong Fan,Jiaren SunPubMed 34411293
Key facts
- Detection method
- Fluorescent
- Sample types
- Suspension cells, Adherent cells
- Assay type
- Cell-based
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TMRM Assay Kit (Mitochondrial Membrane Potential) ab228569 is a complete kit for TMRM staining of cell cultures. Readout by flow cytometry, fluorescence plate reader, or fluorescence microscopy (Ex/Em 548/573 nm). Contains TMRM reagent, buffer and control.
Key facts
- Detection method
- Fluorescent
- Sample types
- Suspension cells, Adherent cells
- Assay type
- Cell-based
- Assay Platform
- Microplate reader, Fluor. microscope, Flow cyt.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- Multi
- Appropriate long-term storage conditions
- Multi
- Storage information
- Please refer to protocols
Notes
TMRM Assay Kit (Mitochondrial Membrane Potential) ab228569 is designed to quantify changes in mitochondrial membrane potential in living cells using flow cytometry, fluorescence plate reader, or fluorescence microscopy. It uses the red-fluorescent probe TMRM (tetramethylrhodamine, methyl ester) that localizes in mitochondria and detects mitochondrial membrane depolarization.
Related assay kits
TMRE is the ethyl ester of tetramethylrhodamine and is a close equivalent of TMRM. TMRE is used about 3 times more than TMRM for mitochondrial membrane potential assays.
TMRE-Mitochondrial Membrane Potential Assay Kit TMRE-Mitochondrial Membrane Potential Assay Kit ab113852 is our most popular assay kit for mitochondrial membrane potential analysis.
TMRM staining is used to monitor mitochondrial function. When accumulated in a negatively charge polarized mitochondria, TMRM fluoresces orange. When mitochondrial membrane potential collapses in apoptotic or metabolically stressed cells, the TMRM reagent is dispersed throughout the cell cytosol and fluorescence levels drop dramatically. This kit provides enough reagents to analyze 5 x 96 wells (500 samples).
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1 product image
Immunocytochemistry/ Immunofluorescence - TMRM Assay Kit (Mitochondrial Membrane Potential) (ab228569)
Human fibroblast cells stained using ab228569 TMRM Assay Kit (Mitochondrial Membrane Potential) (200 nM TMRM).
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- Visit the general protocols
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