Total Antioxidant Capacity Assay Kit

Total Antioxidant Capacity Assay Kit ab65329 can measure either the combination of both small molecule antioxidants and proteins, or small molecules alone in the presence of our proprietary Protein Mask.

In the total antioxidant capacity assay protocol, the Cu2⁺ ion is converted to Cu⁺ by both small molecule and protein antioxidants. The Protein Mask prevents Cu2⁺ reduction by proteins, enabling the analysis of only the small molecule antioxidants. The reduced Cu⁺ ion is chelated with a colorimetric probe giving a broad absorbance peak around 570 nm, proportional to the total antioxidant capacity.

Total antioxidant capacity assay protocol summary:
- add protein mask to samples if only measuring small molecule total antioxidant capacity
- add samples and standards to wells
- add Cu2⁺ solution and incubate for 90 min at room temp
- analyze with microplate reader

Antioxidants play an important role in preventing the formation of, and scavenging of, free radicals and other oxidizing species. There are three categories of antioxidant species: enzyme systems (GSH reductase, catalase, peroxidase, etc.), small molecules (ascorbate, uric acid, GSH, vitamin E, etc.) and proteins (albumin, transferrin, etc.). Trolox is used to standardize antioxidants, with all other antioxidants being measured in Trolox equivalents. Measurement of the combined non-enzymatic antioxidant capacity of biological fluids and other samples provides an indication of the overall capability to counteract reactive oxygen species (ROS), resist oxidative damage and combat oxidative stress-related diseases.**Related products**Review the to learn about more assays for oxidative stress.