AB155891

Total Carbohydrate Assay Kit - Quantification

Name: Total Carbohydrate Assay Kit - Quantification (ab155891) | Abcam
Brand: Abcam Limited
SKU: AB155891
Price: 695 USD
Availability: InStock
Rating: 5 (2 reviews)

(2 Reviews)

(8 Publications)

Total Carbohydrate Assay (ab155891) is a simple, sensitive and robust method of detecting virtually all carbohydrates.

4 Images

Functional Studies - Total Carbohydrate Assay Kit - Quantification (AB155891)

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Functional Studies - Total Carbohydrate Assay Kit - Quantification (AB155891)

Carbohydrates measured in orange juice and caster sugar. Background signal subtracted, duplicates; +/- SD.

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Lab

Functional Studies - Total Carbohydrate Assay Kit - Quantification (AB155891)

Carbohydrates in food items and mouse tissue lysates, shown as mg per mg of extracted protein (duplicates; +/- SD).

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Supplier Data

Functional Studies - Total Carbohydrate Assay Kit - Quantification (AB155891)

D-Glucose standard curve generated using the method described in the protocol bookelt.

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Lab

Functional Studies - Total Carbohydrate Assay Kit - Quantification (AB155891)

Carbohydrates from lysed non-differentiated or adipose differentiated 3T3L1 cells (duplicates; +/- SD). Cells were differentiated using a mixture of small molecules : ab120840, ab120743 and ab123768.

Key facts

Detection method

Colorimetric

Sample types

Suspension cells, Tissue Extracts, Adherent cells, Food samples, Drinks

Assay type

Quantitative

Assay time

30m

Assay Platform

Microplate reader

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Product details

Total Carbohydrate Assay (ab155891) is a simple, sensitive and robust method of detecting virtually all carbohydrates.

How the assay works
The carbohydrate assay protocol is based on the phenol - sulfuric acid assay. In the assay, polysaccharides (mono, di, tri, etc.) and their derivatives, in the presence of sulfuric acid, are hydrolyzed to monomers and converted to furfural or hydroxyfurfural. These react with the developer to form a chromogen that can be quantified by measuring the absorbance at 490 nm.

Total carbohydrate assay protocol summary:
- Add samples and glucose standards to wells
- Add concentrated sulfuric acid and incubate for 15 min at 90°C
- Add developer and incubate for 5 min

How other researchers are using ab155891
Total Carbohydrate Assay Kit ab155901 has been used in a variety of sample type including:
SHK-1 cell line ¹
Blood samples from mice with metabolic syndrome ²
Lattuce seeds extracts ³
References: 1-Martinez D et al. 2022, 2-Greco C et al. 2020, 3-Rawashdeh Y et al. 2020

Related and recommended products
See an alternative kit to quantify carbohydrates:
High Sensitivity Carbohydrate Assay Kit (Colorimetric) ab284543

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What's included?

{ "values": { "100Test": { "sellingSize": "100 Test", "publicAssetCode":"ab155891-100Test", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"Assay Buffer 7", "number":"AB155891-CMP02", "productcode":"" }, { "size":"1 x 0.2 mL", "name":"D-Glucose Standard", "number":"AB155891-CMP01", "productcode":"" }, { "size":"1 x 3 mL", "name":"Developer Solution IV", "number":"AB155891-CMP03", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

Please refer to protocols

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Product protocols

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Target data

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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Microbiology spectrum 10:e0351122 PubMed36445133

2022

A Novel XRE-Type Regulator Mediates Phage Lytic Development and Multiple Host Metabolic Processes in Pseudomonas aeruginosa.

Applications

Unspecified application

Species

Unspecified reactive species

Xiang Long,Xiaolong Wang,Daqing Mao,Weihui Wu,Yi Luo

Applied and environmental microbiology 88:e0106822 PubMed36073944

2022

RecT Affects Prophage Lifestyle and Host Core Cellular Processes in Pseudomonas aeruginosa.

Applications

Unspecified application

Species

Unspecified reactive species

Xiang Long,Hanhui Zhang,Xiaolong Wang,Daqing Mao,Weihui Wu,Yi Luo

Frontiers in immunology 13:849752 PubMed35493529

2022

PAMPs of Trigger the Transcription of Genes Involved in Nutritional Immunity in a Salmon Macrophage-Like Cell Line.

Applications

Unspecified application

Species

Unspecified reactive species

Danixa Pamela Martínez,Cristian Oliver,Natacha Santibañez,José Leonardo Coronado,Ricardo Oyarzún-Salazar,Ricardo Enriquez,Luis Vargas-Chacoff,Alex Romero

Applied and environmental microbiology 88:e0151421 PubMed34788068

2021

Selection for Phage Resistance Reduces Virulence of Shigella flexneri.

Applications

Unspecified application

Species

Unspecified reactive species

Kaitlyn E Kortright,Rachel E Done,Benjamin K Chan,Valeria Souza,Paul E Turner

Scientific reports 10:12915 PubMed32737396

2020

A non-pharmacological therapeutic approach in the gut triggers distal metabolic rewiring capable of ameliorating diet-induced dysfunctions encompassed by metabolic syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Carolina Magdalen Greco,Stefano Garetto,Emilie Montellier,Yu Liu,Siwei Chen,Pierre Baldi,Paolo Sassone-Corsi,Jacopo Lucci

Heliyon 6:e03983 PubMed32509982

2020

Biological interaction levels of zinc oxide nanoparticles; lettuce seeds as case study.

Applications

Unspecified application

Species

Unspecified reactive species

Rabeah Yousef Rawashdeh,Amal Mohammad Harb,Asma Mahmoud AlHasan

Cell reports 26:969-983.e4 PubMed30673618

2019

Maternal Nicotinamide Riboside Enhances Postpartum Weight Loss, Juvenile Offspring Development, and Neurogenesis of Adult Offspring.

Applications

Unspecified application

Species

Unspecified reactive species

Po Hien Ear,Ankita Chadda,Serena B Gumusoglu,Mark S Schmidt,Sophia Vogeler,Johnny Malicoat,Jacob Kadel,Michelle M Moore,Marie E Migaud,Hanna E Stevens,Charles Brenner

Environmental microbiology reports 11:227-235 PubMed30298689

2018

Antarctic heterotrophic bacterium Hymenobacter nivis P3 displays light-enhanced growth and expresses putative photoactive proteins.

Applications

Unspecified application

Species

Unspecified reactive species

Mia Terashima,Keisuke Ohashi,Taichi E Takasuka,Hisaya Kojima,Manabu Fukui

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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