Total Carbohydrate Assay (ab155891) is a simple, sensitive and robust method of detecting virtually all carbohydrates.
Images
Publications
Filter by
- Microbiology spectrum 10:e03511222022A Novel XRE-Type Regulator Mediates Phage Lytic Development and Multiple Host Metabolic Processes in Pseudomonas aeruginosa.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiang Long,Xiaolong Wang,Daqing Mao,Weihui Wu,Yi LuoPubMed 36445133
- Applied and environmental microbiology 88:e01068222022RecT Affects Prophage Lifestyle and Host Core Cellular Processes in Pseudomonas aeruginosa.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiang Long,Hanhui Zhang,Xiaolong Wang,Daqing Mao,Weihui Wu,Yi LuoPubMed 36073944
- Frontiers in immunology 13:8497522022PAMPs of Trigger the Transcription of Genes Involved in Nutritional Immunity in a Salmon Macrophage-Like Cell Line.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDanixa Pamela Martínez,Cristian Oliver,Natacha Santibañez,José Leonardo Coronado,Ricardo Oyarzún-Salazar,Ricardo Enriquez,Luis Vargas-Chacoff,Alex RomeroPubMed 35493529
- Applied and environmental microbiology 88:e01514212021Selection for Phage Resistance Reduces Virulence of Shigella flexneri.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKaitlyn E Kortright,Rachel E Done,Benjamin K Chan,Valeria Souza,Paul E TurnerPubMed 34788068
- Scientific reports 10:129152020A non-pharmacological therapeutic approach in the gut triggers distal metabolic rewiring capable of ameliorating diet-induced dysfunctions encompassed by metabolic syndrome.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCarolina Magdalen Greco et. al.PubMed 32737396
- Heliyon 6:e039832020Biological interaction levels of zinc oxide nanoparticles; lettuce seeds as case study.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRabeah Yousef Rawashdeh et. al.PubMed 32509982
- Cell reports 26:969-983.e42019Maternal Nicotinamide Riboside Enhances Postpartum Weight Loss, Juvenile Offspring Development, and Neurogenesis of Adult Offspring.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPo Hien Ear,Ankita Chadda,Serena B Gumusoglu,Mark S Schmidt,Sophia Vogeler,Johnny Malicoat,Jacob Kadel,Michelle M Moore,Marie E Migaud,Hanna E Stevens,Charles BrennerPubMed 30673618
- Environmental microbiology reports 11:227-2352018Antarctic heterotrophic bacterium Hymenobacter nivis P3 displays light-enhanced growth and expresses putative photoactive proteins.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMia Terashima,Keisuke Ohashi,Taichi E Takasuka,Hisaya Kojima,Manabu FukuiPubMed 30298689
Key facts
- Detection method
Colorimetric
- Sample types
Tissue, Suspension cells, Tissue Extracts, Adherent cells
- Assay type
Quantitative
- Assay time
30m
What's included?
Recommended products
Total Carbohydrate Assay (ab155891) is a simple, sensitive and robust method of detecting virtually all carbohydrates.
Key facts
- Detection method
Colorimetric
- Sample types
Tissue, Suspension cells, Tissue Extracts, Adherent cells
- Assay type
Quantitative
- Assay time
30m
- Assay Platform
Microplate reader
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
+4°C
- Storage information
+4°C
Notes
Total Carbohydrate Assay (ab155891) is a simple, sensitive and robust method of detecting virtually all carbohydrates.
The carbohydrate assay protocol is based on the phenol - sulfuric acid assay. In the assay, polysaccharides (mono, di, tri, etc.) and their derivatives, in the presence of sulfuric acid, are hydrolyzed to monomers and converted to furfural or hydroxyfurfural. These react with the developer to form a chromogen that can be quantified by measuring the absorbance at λ = 490 nm.
The total carbohydrate assay kit can detect most forms of carbohydrates, including simple and complex saccharides, glycans, glycoproteins and glycolipids.
Total carbohydrate assay protocol summary:
- add samples and glucose standards to wells
- add concentrated sulfuric acid and incubate for 15 min at 90°C
- add developer and incubate for 5 min
- analyze with a microplate reader
This product is manufactured by BioVision, an Abcam company and was previously called K645 Total Carbohydrate Colorimetric Assay Kit. K645-100 is the same size as the 100 test size of ab155891.
Carbohydrates play important structural as well as chemical roles in all living systems. Detection of total carbohydrates, therefore, has wide applications.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
Functional Studies - Total Carbohydrate Assay Kit - Quantification (ab155891)
Carbohydrates in food items and mouse tissue lysates, shown as mg per mg of extracted protein (duplicates; +/- SD).
Functional Studies - Total Carbohydrate Assay Kit - Quantification (ab155891)
Carbohydrates measured in orange juice and caster sugar. Background signal subtracted, duplicates; +/- SD.
Functional Studies - Total Carbohydrate Assay Kit - Quantification (ab155891)
Carbohydrates from lysed non-differentiated or adipose differentiated 3T3L1 cells (duplicates; +/- SD). Cells were differentiated using a mixture of small molecules: IBMX, Non-specific cAMP and cGMP inhibitor ab120840, Dexamethasone, anti-inflammatory glucocorticoid ab120743 and Recombinant human Insulin protein (Active) ab123768.
Functional Studies - Total Carbohydrate Assay Kit - Quantification (ab155891)
D-Glucose standard curve generated using the method described in the protocol bookelt.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com