Total Collagen Assay Kit (Perchlorate-Free) ab222942 provides a quick and convenient method to detect collagen.
- Detection limit as low as 0.5 µg of collagen
- Does not require the use of hazardous perchloric acid for hydrolysates, removing any special handling and waste-disposal protocols
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- Journal of cellular physiology 239:e311912024Transplantation of beige adipose organoids fabricated using adipose acellular matrix hydrogel improves metabolic dysfunction in high-fat diet-induced obesity and type 2 diabetes mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYuping Quan,Jian Li,Junrong Cai,Yunjun Liao,Yuteng Zhang,Feng LuPubMed 38219044
- Biology 12:2023Dimethyl Sulfoxide Inhibits Bile Acid Synthesis in Healthy Mice but Does Not Protect Mice from Bile-Acid-Induced Liver Damage.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXi Chen,Huiqiao Li,Yu'e Liu,Jing Qi,Bingning Dong,Shixia Huang,Shangang Zhao,Yi ZhuPubMed 37626991
- Clinical oral investigations 27:5031-50402023Characterization of the soft-tissue wall lining residual periodontal pockets and implications in periodontal wound healing.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEvangelia Gousopoulou,Athina Bakopoulou,Dimitrios Laskaris,Epameinondas Gousopoulos,Danae A ApatzidouPubMed 37486381
- The European respiratory journal 61:2023Regulating the cell shift of endothelial cell-like myofibroblasts in pulmonary fibrosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiuju Wu,Daoqin Zhang,Xiaojing Qiao,Li Zhang,Xinjiang Cai,Jaden Ji,Jocelyn A Ma,Yan Zhao,John A Belperio,Kristina I Boström,Yucheng YaoPubMed 36758986
- Redox biology 59:1025942023Diminished α7 nicotinic acetylcholine receptor (α7nAChR) rescues amyloid-β induced atrial remodeling by oxi-CaMKII/MAPK/AP-1 axis-mediated mitochondrial oxidative stress.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJikai Zhao,Liming Yu,Xiaodong Xue,Yinli Xu,Tao Huang,Dengyue Xu,Zhishang Wang,Linyu Luo,Huishan WangPubMed 36603528
- Frontiers in bioengineering and biotechnology 10:8840692022Characterization of an Innovative Biomaterial Derived From Human Wharton's Jelly as a New Promising Coating for Tissue Engineering Applications.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAdrien Fayon,Deborah Helle,Gregory Francius,Jean-Baptiste Vincourt,Véronique Regnault,Dominique Dumas,Patrick Menu,Reine El OmarPubMed 35769101
- Acta physiologica (Oxford, England) 235:e138282022Increased COX-2 after ureter obstruction attenuates fibrosis and is associated with EP receptor upregulation in mouse and human kidney.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSigne S Tofteng,Line Nilsson,Amalie K Mogensen,Rikke Nørregaard,Rolf Nüsing,Mikhail Diatchikhine,Lars Lund,Claus Bistrup,Boye L Jensen,Kirsten MadsenPubMed 35543087
- Molecular metabolism 61:1014942022Hmgcs2-mediated ketogenesis modulates high-fat diet-induced hepatosteatosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShaza Asif,Ri Youn Kim,Thet Fatica,Jordan Sim,Xiaoling Zhao,Yena Oh,Alix Denoncourt,Angela C Cheung,Michael Downey,Erin E Mulvihill,Kyoung-Han KimPubMed 35421611
- Frontiers in bioengineering and biotechnology 10:7960762022Pulmonary Visceral Pleura Biomaterial: Elastin- and Collagen-Based Extracellular Matrix.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiao Lu,Ling Han,Ghassan S KassabPubMed 35433658
- Journal of nanobiotechnology 19:2642021Injectable exosome-functionalized extracellular matrix hydrogel for metabolism balance and pyroptosis regulation in intervertebral disc degeneration.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHongyuan Xing,Zengjie Zhang,Qijiang Mao,Chenggui Wang,Youlong Zhou,Xiaopeng Zhou,Liwei Ying,Haibin Xu,Shaojun Hu,Ning ZhangPubMed 34488795
Key facts
- Detection method
- Colorimetric
- Sample types
- Urine, Tissue Homogenate, Serum
- Assay type
- Quantitative
- Reactive species
- Mammals
- Range
- 0.5 - 10 µg
- Assay time
- 3h
Associated Products
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Target data
Function
Anchors the catalytic subunits of asymmetric AChE to the synaptic basal lamina.
Alternative names
Acetylcholinesterase collagenic tail peptide, AChE Q subunit, Acetylcholinesterase-associated collagen, Colq
What's included?
Recommended products
Total Collagen Assay Kit (Perchlorate-Free) ab222942 provides a quick and convenient method to detect collagen.
- Detection limit as low as 0.5 µg of collagen
- Does not require the use of hazardous perchloric acid for hydrolysates, removing any special handling and waste-disposal protocols
Key facts
- Detection method
- Colorimetric
- Sample types
- Urine, Tissue Homogenate, Serum
- Assay type
- Quantitative
- Reactive species
- Mammals
- Range
- 0.5 - 10 µg
- Assay time
- 3h
- Assay Platform
- Microplate reader
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Please refer to protocols
Notes
Total collagen assay principle
The total collagen assay protocol is based on the total alkaline/acid hydrolysis of samples to breakdown the collagen trihelix peptide into single amino acids and yield free Hydroxyproline. The released hydroxyproline is oxidized to form a reaction intermediate, which further in reaction forms brightly-colored chromophore that can be easily detected at OD 560 nm.
The total collagen assay uses an identical assay principle and assay components to our hydroxyproline assay kit Hydroxyproline Assay Kit (Colorimetric) ab222941, except that the standard curve of this assay is constructed from collagen itself (and that collagen is treated in an identical manner to samples, undergoing alkaline hydrolysis at 120C). This makes this assay a more direct way to quantify collagen levels.
The assay employs a proprietary developer solution to accurately measure collagen in hydrolysates without the use of hazardous perchloric acid, removing the need for any special handling and waste-disposal protocols.
Total Collagen assay kit protocol summary:
- Hydrolyze samples and standard with concentrated NaOH for 1 hr at 120°C
- Cool on ice and neutralize with concentrated HCL
- Centrifuge at 10,000g for 5 min to pellet debris
- Add samples and standards to wells
- Evaporate wells to dryness at 65°C
- Add oxidation mix and incubate for 20 min
- Add developer and incubate for 5 min
- Add DMAB concentrate and incubate for 45 min at 65°C
- Analyze absorbance at 560 nm with a microplate reader
How other researchers are using
Total Collagen Assay Kit (Perchlorate-Free) has been used in a variety of sample type including:
- Mouse liver samples1
- Mesenchymal stem cell culture 2
References:
1- Chen X al. 2023
2 Gousopoulou X et al. 2023
This product is manufactured by BioVision, an Abcam company and was previously called K406 Total Collagen Assay Kit (Perchlorate-Free). K406-100 is the same size as the 100 test size of ab222942.
Collagen is the most abundant insoluble protein found in the extracellular matrix and connective tissues. It can be found in skin, tendons, bone, cartilage, muscle, vitreous humor and ligaments, among other tissues. There are more than sixteen - well characterized types of collagens, but types I, II and III collagen comprise more than 80% content in mammals.
The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
Functional Studies - Total Collagen Assay Kit (Perchlorate-Free) (ab222942)
Estimation of total collagen content in rat tissues. Rat leg muscle, spleen, heart and lung samples were prepared followind the sample preparation procedure described in the protocol (homegenized in ddH2O, hydrolyzed with 10 N NaOH for 1 hour at 120°C and neutralized with 10 N HCl). For each sample, 10 μL of the final neutralized hydrolysate was assayed. Total Collagen levels (calculated as μg collagen/mg wet tissue) for the samples were: 13.80 +- 0.73 ug/mg for muscle, 3.77 +- 0.54 ug/mg for spleen, 4.55 +- 0.81 ug/mg for heart and 12.51 +- 1.27 ug/mg for lung. Data are mean ± SEM of 3-4 replicates.
Functional Studies - Total Collagen Assay Kit (Perchlorate-Free) (ab222942)
Typical hydrolyzed Collagen I standard calibration curve.
Functional Studies - Total Collagen Assay Kit (Perchlorate-Free) (ab222942)
Correlation between collagen standard curve absorbance values obtained using a classical perchlorate-based assay kit and perchlorate-free Total Collagen Assay Kit (Colorimetric) (ab222942).
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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