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This Total Glycosaminoglycans Assay Kit (ab289842, K2085) can measure the total amount of Glycosaminoglycans (GAGs) including sulfated GAGs such as chondroitin sulfate, heparan sulfate etc.

Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.

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Images

Functional Studies - Total Glycosaminoglycans Assay Kit (Colorimetric) (AB289842), expandable thumbnail
  • Functional Studies - Total Glycosaminoglycans Assay Kit (Colorimetric) (AB289842), expandable thumbnail
  • Functional Studies - Total Glycosaminoglycans Assay Kit (Colorimetric) (AB289842), expandable thumbnail

Key facts

Detection method

Colorimetric

Sample types

Food samples, Saliva, Tissue

Assay type

Quantitative

Reactive species

Mammals

Targets

Hyaluronic acid

What's included?

100 Test
Components
GAG Assay Buffer
1 x 25 mL
GAG Probe
1 x 25 mL
GAG Standard
1 x 4 mL

Recommended products

This Total Glycosaminoglycans Assay Kit (ab289842, K2085) can measure the total amount of Glycosaminoglycans (GAGs) including sulfated GAGs such as chondroitin sulfate, heparan sulfate etc.

Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.

Key facts

Detection method

Colorimetric

Sample types

Food samples, Saliva, Tissue

Assay type

Quantitative

Reactive species

Mammals

Assay Platform

Microplate

General recovery

90%

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

Notes

This Total Glycosaminoglycans Assay Kit (ab289842, K2085) can measure the total amount of Glycosaminoglycans (GAGs) including sulfated GAGs such as chondroitin sulfate, heparan sulfate etc. and non-sulfated GAGs such as hyaluronic acid in various biological samples. In this assay, GAGs interacts with a specific probe to form a colored product that is measured by absorbance at 400 nm. The colored signal is directly proportional to the GAG concentration in the sample. The kit offers a simple, rapid, sensitive and convenient way to measure the total amount of GAGs in various sample types. It can detect as low as 1 μg of chondroitin sulfate or hyaluronic acid under the assay conditions.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Functional Studies - Total Glycosaminoglycans Assay Kit (Colorimetric) (ab289842), expandable thumbnail

    Functional Studies - Total Glycosaminoglycans Assay Kit (Colorimetric) (ab289842)

    Total Glycosaminoglycans Assay Kit (Colorimetric).

    Example GAG Standard Curve. A new standard curve should be generated alongside every assay.

  • Functional Studies - Total Glycosaminoglycans Assay Kit (Colorimetric) (ab289842), expandable thumbnail

    Functional Studies - Total Glycosaminoglycans Assay Kit (Colorimetric) (ab289842)

    Total Glycosaminoglycans Assay Kit (Colorimetric).

    Amount of GAGs detected in saliva (50 μl) and orange juice (10 μl) samples. >90% recovery was observed when spiked with 2 μg of GAG.

  • Functional Studies - Total Glycosaminoglycans Assay Kit (Colorimetric) (ab289842), expandable thumbnail

    Functional Studies - Total Glycosaminoglycans Assay Kit (Colorimetric) (ab289842)

    Total Glycosaminoglycans Assay Kit (Colorimetric).

    Amount of GAGs detected in banana and rat brain samples. Assay was performed according to the assay protocol.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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