Total Iron-Binding Capacity (TIBC) and Serum Iron Assay Kit ab239715 uses an addition-only, ferrozine-based method with four 10-min incubation steps. Readout on any colorimetric (570 nm) plate reader.
- quantify free iron, serum iron / transferrin-bound iron, and total iron-binding capacity
- Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
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- Biomedicines 11:2023High-Altitude Hypoxia Induces Excessive Erythrocytosis in Mice via Upregulation of the Intestinal /Iron-Metabolism Pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSisi Zhou,Jun Yan,Kang Song,Ri-Li GePubMed 38001992
- Cardiovascular toxicology 23:295-3042023Suberosin Alleviates Thiazolidinedione-Induced Cardiomyopathy in Diabetic Rats by Inhibiting Ferroptosis via Modulation of ACSL4-LPCAT3 and PI3K-AKT Signaling Pathways.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShabnoor Iqbal,Farhat Jabeen,Ivan Kahwa,Timothy OmaraPubMed 37676618
- Proceedings of the National Academy of Sciences of the United States of America 120:e22126441202023Hepatic SEL1L-HRD1 ER-associated degradation regulates systemic iron homeostasis via ceruloplasmin.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPattaraporn Thepsuwan,Asmita Bhattacharya,Zhenfeng Song,Stephen Hippleheuser,Shaobin Feng,Xiaoqiong Wei,Nupur K Das,Mariana Sierra,Juncheng Wei,Deyu Fang,Yu-Ming M Huang,Kezhong Zhang,Yatrik M Shah,Shengyi SunPubMed 36595688
- Fluids and barriers of the CNS 19:492022Regulation of brain iron uptake by apo- and holo-transferrin is dependent on sex and delivery protein.Applications:Unspecified applicationReactive species:Unspecified reactive speciesStephanie L Baringer,Elizabeth B Neely,Kondaiah Palsa,Ian A Simpson,James R ConnorPubMed 35689283
- Annals of palliative medicine 11:1687-16992022Intravenous iron injection does not impair liver function in a rat model of cirrhosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJi-Hye Kwon,Sukyoung Her,Seungwon Lee,Sangbin Han,Mi Sook Gwak,Gaab Soo Kim,Tae Soo Hahm,Hyun Sung Cho,Justin S KoPubMed 35016521
- Cell research 30:119-1322019Transferrin plays a central role in coagulation balance by interacting with clotting factors.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaopeng Tang,Zhiye Zhang,Mingqian Fang,Yajun Han,Gan Wang,Sheng Wang,Min Xue,Yaxiong Li,Li Zhang,Jian Wu,Biqing Yang,James Mwangi,Qiumin Lu,Xiaoping Du,Ren LaiPubMed 31811276
Key facts
- Detection method
- Colorimetric
- Sample types
- Heparin Plasma, Serum
Target data
What's included?
Recommended products
Total Iron-Binding Capacity (TIBC) and Serum Iron Assay Kit ab239715 uses an addition-only, ferrozine-based method with four 10-min incubation steps. Readout on any colorimetric (570 nm) plate reader.
- quantify free iron, serum iron / transferrin-bound iron, and total iron-binding capacity
- Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
Key facts
- Detection method
- Colorimetric
- Sample types
- Heparin Plasma, Serum
- Assay Platform
- Microplate reader
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
Total Iron-Binding Capacity (TIBC) and Serum Iron Assay Kit (ab239715) provides a simple, sensitive, and high-throughput adaptable approach to detect physiological Total Iron-Binding Capacity (TIBC) and Serum Iron. Those values indicate the requisite iron for transferrin saturation and Serum Iron respectively. Those measurements can be used to detect and monitor transferrin saturation and also iron-deficiency anemia and chronic inflammatory diseases.
Total Iron-Binding Capacity (TIBC) and Serum Iron assay principles
In the combined Total Iron-Binding Capacity (TIBC) and Serum Iron assay kit, iron levels are measured using a ferrozine-based method.
To measure the levels of free iron, samples are treated with a reducing agent to convert any ferric iron (Fe3+) to form ferrous iron (Fe2+). Ferrozine then reacts with ferrous iron in the sample to form a complex measured by absorbance at 570 nm.
To measure the total level of free iron + transferrin bound iron, an acidic buffer is also used to release iron from iron carrier proteins, before the measurement of iron levels.
To measure the total iron binding capacity, an iron solution is added to saturate the iron-binding capacity of the sample. Then the same process is used to measure free iron and the total level of free iron + transferrin bound iron.
Serum Iron is defined as the amount of iron in serum that is bound to transferrin. It is calculated as the total level of free iron + transferrin bound iron, less the amount of free iron.
Total Iron-Binding Capacity (TIBC) is calculated as the total level of free iron + transferrin bound iron after saturation with the iron solution, less the amount of free iron.
Getting the best performance from ab239715
For optimal enzymatic activity samples should not contain EDTA or other chelating agents.
The ab239715 assay is not compatible with plasma samples collected with EDTA or citrate anticoagulants. Plasma samples collected in heparin are compatible.
This product was previously called K392 Biovision Total Iron-Binding Capacity (TIBC) and Serum Iron Assay Kit (Colorimetric). Abcam acquired Biovision in 2021.
The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.
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2 product images
Functional Studies - Total Iron-Binding Capacity (TIBC) and Serum Iron Assay Kit (ab239715)
Iron standard Curve generated using the kit protocol.
Functional Studies - Total Iron-Binding Capacity (TIBC) and Serum Iron Assay Kit (ab239715)
Serum Iron and TIBC determination of serum. Assays were performed following the kit protocol.
Downloads
Product protocols
- Visit the general protocols
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