Total Polyamine Assay Kit (ab239728) enables the rapid determination of polyamine concentration in biological samples.
Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
Fluorescent
Tissue, Other biological fluids
Total Polyamine Assay Kit (ab239728) enables the rapid determination of polyamine concentration in biological samples.
Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
Fluorescent
Tissue, Other biological fluids
Microplate reader
Blue Ice
-20°C
-20°C
-20°C
Total Polyamine Assay Kit (ab239728) enables the rapid determination of polyamine concentration in biological samples. A selective enzyme mix acts on polyamines, generating hydrogen peroxide that is then reacted with a fluorometric probe (Ex/Em = 535/587 nm) to yield a signal proportional to the amount of polyamine present. The kit includes a proprietary Sample Clean-Up reagent for pre-treating samples in order to eliminate common metabolites found in biological samples that may interfere with the assay or increase sample background. The assay is rapid, simple, and high throughput compatible, and can detect polyamine concentrations as low as 0.1 μM in tissue lysates and other samples such as saliva.
This product is manufactured by BioVision, an Abcam company and was previously called K475 Total Polyamine Assay Kit (Fluorometric). K475-100 is the same size as the 100 test size of ab239728.
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Full details and terms and conditions can be found here:
Terms & Conditions.
Determination of total polyamine concentration in saliva and intestinal tissue lysate.
Determination of total polyamine concentration in saliva (determined to be 43.0 +/- 8.9 μM) and intestinal tissue lysate (63.9 +/- 16.0 nmole/mg protein). For this experiment, 100 mg rat intestine was homogenized and prepared according to the kit protocol. Saliva (2 μL) and intestinal lysate were treated with Sample Clean-Up Mix. Values are mean ± standard deviation of at least three independent determinations.
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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