Total Polyamine Assay Kit (ab239728) enables the rapid determination of polyamine concentration in biological samples.
Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
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- PloS one 18:e02894762023The effects of a comparatively higher dose of 1000 mg/kg/d of oral L- or D-arginine on the L-arginine metabolic pathways in male Sprague-Dawley rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDain Raina Kim,Sarah Martin,Kaushik DesaiPubMed 37527267
- Oxidative medicine and cellular longevity 2022:80333532022Rebalance of the Polyamine Metabolism Suppresses Oxidative Stress and Delays Senescence in Nucleus Pulposus Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHui Che,Cheng Ma,He Li,Fenglei Yu,Yifan Wei,Hailong Chen,Jun Wu,Yongxin RenPubMed 35178160
Key facts
- Detection method
Fluorescent
- Sample types
Tissue, Other biological fluids
What's included?
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Total Polyamine Assay Kit (ab239728) enables the rapid determination of polyamine concentration in biological samples.
Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
Key facts
- Detection method
Fluorescent
- Sample types
Tissue, Other biological fluids
- Assay Platform
Microplate reader
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
-20°C
- Appropriate long-term storage conditions
-20°C
- Storage information
-20°C
Notes
Total Polyamine Assay Kit (ab239728) enables the rapid determination of polyamine concentration in biological samples. A selective enzyme mix acts on polyamines, generating hydrogen peroxide that is then reacted with a fluorometric probe (Ex/Em = 535/587 nm) to yield a signal proportional to the amount of polyamine present. The kit includes a proprietary Sample Clean-Up reagent for pre-treating samples in order to eliminate common metabolites found in biological samples that may interfere with the assay or increase sample background. The assay is rapid, simple, and high throughput compatible, and can detect polyamine concentrations as low as 0.1 μM in tissue lysates and other samples such as saliva.
This product is manufactured by BioVision, an Abcam company and was previously called K475 Total Polyamine Assay Kit (Fluorometric). K475-100 is the same size as the 100 test size of ab239728.
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Functional Studies - Total Polyamine Assay Kit (ab239728)
Functional Studies - Total Polyamine Assay Kit (ab239728)
Determination of total polyamine concentration in saliva and intestinal tissue lysate.
Determination of total polyamine concentration in saliva (determined to be 43.0 +/- 8.9 μM) and intestinal tissue lysate (63.9 +/- 16.0 nmole/mg protein). For this experiment, 100 mg rat intestine was homogenized and prepared according to the kit protocol. Saliva (2 μL) and intestinal lysate were treated with Sample Clean-Up Mix. Values are mean ± standard deviation of at least three independent determinations.
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