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AB239728

Total Polyamine Assay Kit

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(4 Publications)

Total Polyamine Assay Kit (ab239728) enables the rapid determination of polyamine concentration in biological samples.

Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
3 Images
Functional Studies - Total Polyamine Assay Kit (AB239728)
  • FuncS

Supplier Data

Functional Studies - Total Polyamine Assay Kit (AB239728)

Determination of total polyamine concentration in saliva and intestinal tissue lysate.

Determination of total polyamine concentration in saliva (determined to be 43.0 +/- 8.9 μM) and intestinal tissue lysate (63.9 +/- 16.0 nmole/mg protein). For this experiment, 100 mg rat intestine was homogenized and prepared according to the kit protocol. Saliva (2 μL) and intestinal lysate were treated with Sample Clean-Up Mix. Values are mean ± standard deviation of at least three independent determinations.

Functional Studies - Total Polyamine Assay Kit (AB239728)
  • FuncS

Supplier Data

Functional Studies - Total Polyamine Assay Kit (AB239728)

Polyamine standard curve

Schematic Diagram - Total Polyamine Assay Kit (AB239728)
  • Schematic Diagram

Supplier Data

Schematic Diagram - Total Polyamine Assay Kit (AB239728)

Representative image of Total Polyamine Assay Kit ab239728

Components shown from left to right :

- OxiRed Probe

- Sample Clean-Up Mix I

- Polyamine Standard

- Developer Solution V

- Converter Mix K

- Total Polyamine Assay Buffer

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit.

Key facts

Detection method

Fluorescent

Sample types

Tissue, Other biological fluids

Assay Platform

Microplate reader

Product details

Total Polyamine Assay Kit (ab239728) enables the rapid determination of polyamine concentration in biological samples. A selective enzyme mix acts on polyamines, generating hydrogen peroxide that is then reacted with a fluorometric probe (Ex/Em = 535/587 nm) to yield a signal proportional to the amount of polyamine present. The kit includes a proprietary Sample Clean-Up reagent for pre-treating samples in order to eliminate common metabolites found in biological samples that may interfere with the assay or increase sample background. The assay is rapid, simple, and high throughput compatible, and can detect polyamine concentrations as low as 0.1 uM in tissue lysates and other samples such as saliva.

Other Notes
This product was previously called K475 Biovision Total Polyamine Assay Kit (Fluorometric). Biovision was acquired by Abcam in 2021.

The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.

What's included?

{ "values": { "100Test": { "sellingSize": "100 Test", "publicAssetCode":"ab239728-100Test", "assetComponentDetails": [ { "size":"1 x 1 Vial", "name":"Sample Clean-Up Mix I", "number":"AB239728-CMP01", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Polyamine Standard", "number":"AB239728-CMP06", "productcode":"" }, { "size":"1 x 25 mL", "name":"Polyamine Assay Buffer", "number":"AB239728-CMP07", "productcode":"" }, { "size":"1 x 0.2 mL", "name":"OxiRed™ Probe", "number":"AB239728-CMP05", "productcode":"AB309437" }, { "size":"1 x 1 Vial", "name":"Developer Solution V", "number":"AB239728-CMP03", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Converter Mix K", "number":"AB239728-CMP04", "productcode":"" } ] } } }

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C

Product protocols

Target data

Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Protein science : a publication of the Protein Society 33:e5199 PubMed39473024

2024

Protein degradation of antizyme depends on the N-terminal degrons.

Applications

Unspecified application

Species

Unspecified reactive species

Ju-Yi Hsieh,Pui-Ying Leong,Yi-Fang Yang,Yi-Liang Liu,Guang-Yaw Liu,Hui-Chih Hung

Cell death and differentiation 31:910-923 PubMed38858548

2024

Anaplastic Lymphoma Kinase signaling stabilizes SLC3A2 expression via MARCH11 to promote neuroblastoma cell growth.

Applications

Unspecified application

Species

Unspecified reactive species

Wei-Yun Lai,Tzu-Po Chuang,Marcus Borenäs,Dan E Lind,Bengt Hallberg,Ruth H Palmer

PloS one 18:e0289476 PubMed37527267

2023

The effects of a comparatively higher dose of 1000 mg/kg/d of oral L- or D-arginine on the L-arginine metabolic pathways in male Sprague-Dawley rats.

Applications

Unspecified application

Species

Unspecified reactive species

Dain Raina Kim,Sarah Martin,Kaushik Desai

Oxidative medicine and cellular longevity 2022:8033353 PubMed35178160

2022

Rebalance of the Polyamine Metabolism Suppresses Oxidative Stress and Delays Senescence in Nucleus Pulposus Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Hui Che,Cheng Ma,He Li,Fenglei Yu,Yifan Wei,Hailong Chen,Jun Wu,Yongxin Ren
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