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- FuncS
Supplier Data
Functional Studies - Total Polyamine Assay Kit (AB239728)
Determination of total polyamine concentration in saliva and intestinal tissue lysate.
Determination of total polyamine concentration in saliva (determined to be 43.0 +/- 8.9 μM) and intestinal tissue lysate (63.9 +/- 16.0 nmole/mg protein). For this experiment, 100 mg rat intestine was homogenized and prepared according to the kit protocol. Saliva (2 μL) and intestinal lysate were treated with Sample Clean-Up Mix. Values are mean ± standard deviation of at least three independent determinations.
- FuncS
Supplier Data
Functional Studies - Total Polyamine Assay Kit (AB239728)
Polyamine standard curve
- Schematic Diagram
Supplier Data
Schematic Diagram - Total Polyamine Assay Kit (AB239728)
Representative image of Total Polyamine Assay Kit ab239728
Components shown from left to right :
- OxiRed Probe
- Sample Clean-Up Mix I
- Polyamine Standard
- Developer Solution V
- Converter Mix K
- Total Polyamine Assay Buffer
Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the kit.
Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit.
Product details
Total Polyamine Assay Kit (ab239728) enables the rapid determination of polyamine concentration in biological samples. A selective enzyme mix acts on polyamines, generating hydrogen peroxide that is then reacted with a fluorometric probe (Ex/Em = 535/587 nm) to yield a signal proportional to the amount of polyamine present. The kit includes a proprietary Sample Clean-Up reagent for pre-treating samples in order to eliminate common metabolites found in biological samples that may interfere with the assay or increase sample background. The assay is rapid, simple, and high throughput compatible, and can detect polyamine concentrations as low as 0.1 uM in tissue lysates and other samples such as saliva.
Other Notes
This product was previously called K475 Biovision Total Polyamine Assay Kit (Fluorometric). Biovision was acquired by Abcam in 2021.
The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.
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Publications (4)
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Protein science : a publication of the Protein Society 33:e5199 PubMed39473024
2024
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Cell death and differentiation 31:910-923 PubMed38858548
2024
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PloS one 18:e0289476 PubMed37527267
2023
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Oxidative medicine and cellular longevity 2022:8033353 PubMed35178160
2022
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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