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Total Polyamine Assay Kit (ab239728) enables the rapid determination of polyamine concentration in biological samples.

Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.

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Images

Functional Studies - Total Polyamine Assay Kit (AB239728), expandable thumbnail
  • Functional Studies - Total Polyamine Assay Kit (AB239728), expandable thumbnail

Publications

Key facts

Detection method

Fluorescent

Sample types

Tissue, Other biological fluids

What's included?

100 Test
Components
Assay Buffer XLII
1 x 25 mL
Converter Enzyme XIII
1 x 1 Vial
Developer Solution V
1 x 1 Vial
OxiRed Probe
1 x 200 µL
Polyamine Standard
1 x 1 Vial
Sample Clean-Up Mix I
1 x 1 Vial

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Total Polyamine Assay Kit (ab239728) enables the rapid determination of polyamine concentration in biological samples.

Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.

Key facts

Detection method

Fluorescent

Sample types

Tissue, Other biological fluids

Assay Platform

Microplate reader

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

-20°C

Notes

Total Polyamine Assay Kit (ab239728) enables the rapid determination of polyamine concentration in biological samples. A selective enzyme mix acts on polyamines, generating hydrogen peroxide that is then reacted with a fluorometric probe (Ex/Em = 535/587 nm) to yield a signal proportional to the amount of polyamine present. The kit includes a proprietary Sample Clean-Up reagent for pre-treating samples in order to eliminate common metabolites found in biological samples that may interfere with the assay or increase sample background. The assay is rapid, simple, and high throughput compatible, and can detect polyamine concentrations as low as 0.1 μM in tissue lysates and other samples such as saliva.

This product is manufactured by BioVision, an Abcam company and was previously called K475 Total Polyamine Assay Kit (Fluorometric). K475-100 is the same size as the 100 test size of ab239728.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Functional Studies - Total Polyamine Assay Kit (ab239728), expandable thumbnail

    Functional Studies - Total Polyamine Assay Kit (ab239728)

  • Functional Studies - Total Polyamine Assay Kit (ab239728), expandable thumbnail

    Functional Studies - Total Polyamine Assay Kit (ab239728)

    Determination of total polyamine concentration in saliva and intestinal tissue lysate.

    Determination of total polyamine concentration in saliva (determined to be 43.0 +/- 8.9 μM) and intestinal tissue lysate (63.9 +/- 16.0 nmole/mg protein). For this experiment, 100 mg rat intestine was homogenized and prepared according to the kit protocol. Saliva (2 μL) and intestinal lysate were treated with Sample Clean-Up Mix. Values are mean ± standard deviation of at least three independent determinations.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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