Skip to main content

Trichrome Stain Kit (Connective Tissue Stain) ab150686 is intended for use in the histological visualization of collagenous connective tissue fibers in tissue sections.

Images

What's included?

100 Test
Components
Acetic Acid Solution (1%)
1 x 125 mL
Aniline Blue Solution
1 x 125 mL
Biebrich Scarlet/Acid Fuchsin Solution
1 x 125 mL
Bouin's Fluid
1 x 125 mL
Phosphomolybdic/Phosphotungstic acid solution
1 x 125 mL
Weigert's Iron, Hematoxylin (A)
1 x 125 mL
Weigert's Iron, Hematoxylin (B)
1 x 125 mL

Recommended products

  1. Loading...
  2. Loading...
  3. Loading...
  4. Loading...

Trichrome Stain Kit (Connective Tissue Stain) ab150686 is intended for use in the histological visualization of collagenous connective tissue fibers in tissue sections.

Storage

Shipped at conditions

Ambient - Can Ship with Ice

Appropriate short-term storage conditions

Ambient

Appropriate long-term storage conditions

Ambient

Storage information

Ambient

Notes

Trichrome Stain Kit (Connective Tissue Stain) ab150686 is intended for use in the histological visualization of collagenous connective tissue fibers in tissue sections.

The trichrome stain kit may be used on formalin-fixed, paraffin-embedded. For frozen sections, it will require optimization by the user.

Trichrome stain protocol summary:
- deparaffinize sections if necessary
- preheat Bouin's Fluid to 54-64°C
- incubate slide in preheated Bouin's Fluid for 60 min and cool for 10 min
- rinse in water
- incubate slide in Weigert's Iron Hematoxylin for 5 min
- rinse in water
- incubate slide in Biebrich Scarlet / Acid Fuchsin solution for 15 min
- rinse in water
- differentiate in phosphomolybdic / phosphotungstic acid solution for 10-15 min
- incubate slide in Aniline Blue solution for 5-10 min
- rinse in water
- incubate slide in acetic acid solution for 3-5 min
- dehydrate, clear and mount

Other products for staining tissue sections

Find more kits and reagents in the special stains guide, or products for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in the IHC kits and reagents guide.

Staining Interpretation

CollagenBlue
Muscle FibersRed
NucleiDark Red to Black/Blue

Control Tissue: Lung, Uterus, Small Intestine, Stomach.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

There was a problem

We can’t download that datasheet. Please try again. If you need help, contact our Customer Services team at technical@abcam.com