Trichrome Stain Kit (Connective Tissue Stain)
5
(5 Reviews)
|
(205 Publications)
- Ready to use solutions- Cited in >200 publications
- Includes complete reagents for Aniline Blue, Biebrich Scarlet/Acid Fuchsin, and Weigert's Iron Hematoxylin trichrome staining
- IHC-P
AbReview
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Trichrome Stain Kit (Connective Tissue Stain) (AB150686)
A paraffin-embedded section of healthy mouse spleen stained with ab150686 - Trichrome Stain Kit (Connective Tissue Stain). The sample was deparaffinized by heating to 60°C for 1 hour and then deparaffinization following a standard protocol. Sections were then fixed with Bouin's solution (included in the kit) for 1 hour at 60°C in humid conditions.
This image is courtesy of an Abreview submitted by Javier Rodriguez
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Trichrome Stain Kit (Connective Tissue Stain) (AB150686)
ab150686 Trichrome Stain Kit (Connective Tissue Stain) staining formalin-fixed-paraffin embedded human muscle.
- IHC-P
Lab
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Trichrome Stain Kit (Connective Tissue Stain) (AB150686)
ab150686 (Trichrome Stain) staining collagen (blue) and muscle fibre (red) in formalin fixed paraffin embedded normal human placenta. Nuclei are counterstained with Weigert's hematoxylin (blue)
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.
Product details
Masson's Trichrome Stain Kit ab150686 for histological staining of collagen, collagen fibers, fibrin, muscles, and erythrocytes. Three stains are used in Masson's trichrome staining: Aniline blue, Biebrich scarlet-acid fuschin, and Weigert’s Hematoxylin. Aniline blue is provided in this kit; in some other protocols a green dye is used to stain collagen instead of Aniline blue.
The trichrome staining protocol used in this kit is designed for use on formalin-fixed, paraffin-embedded tissue sections. For frozen sections, it will require optimization by the user.
Masson's Trichrome staining protocol summary
- Deparaffinize sections if necessary
- Preheat Bouin's Fluid to 54-64°C
- Incubate slide in preheated Bouin's Fluid for 60 min and cool for 10 min
- Rinse in water
- Incubate slide in Weigert's Iron Hematoxylin for 5 min
- Rinse in water
- Incubate slide in Biebrich Scarlet / Acid Fuchsin solution for 15 min
- Rinse in water
- Differentiate in phosphomolybdic / phosphotungstic acid solution for 10-15 min
- Incubate slide in Aniline Blue solution for 5-10 min
- Rinse in water
- Incubate slide in acetic acid solution for 3-5 min
- Dehydrate, clear and mount
Staining Interpretation
| Collagen | Blue (Aniline Blue) |
| Muscle Fibers | Red (Biebrich scarlet-acid fuschin) |
| Nuclei | Dark Red to Black/Blue (Weigert’s Hematoxylin) |
Control Tissue: Lung, Uterus, Small Intestine, Stomach.
Related and recommended products
See below other connective tissue stains to highlight and identify different types of connective tissue in tissue samples with different dyes:
- Picro Sirius Red Stain Kit (Connective Tissue Stain) ab150681
- Phosphotungstic Acid Hematoxylin (PTAH) Stain Kit (Connective Tissue Stain) ab150683
- Reticulum Stain Kit (Connective Tissue Stain) ab150684
How other researchers use Masson's Trichrome Staining
Based on a brief survey of publications using Trichrome Stain Kit ab150686, Masson's Trichrome staining is most often used to stain for fibrosis and collagen levels in liver, heart, and lung. It is also often used with pancreas, bladder, kidney, skin, adipose tissue, and vascular tissue.
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Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Collagenous fibers contribute to the mechanical framework of connective tissues enabling them to resist stretching and deformation. They are part of larger complexes that include elastin and proteoglycans working together to maintain tissue integrity and function. These fibers interact with other cellular components through specific receptors like integrins which mediate signals for cellular processes including differentiation adhesion and migration.
Pathways
Collagenous connective tissue fibers play roles in wound healing and tissue remodeling. They participate in pathways involving matrix metalloproteinases (MMPs) which regulate the degradation of extracellular matrix components. The collagen synthesis and cross-linking process often links with proteins such as fibronectin and laminin which influence cell behavior and tissue repair mechanisms.
Product protocols
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Publications (205)
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