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AB178780

Triglyceride Assay Kit (Fluorometric, Reducing Samples)

3

(1 Review)

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(20 Publications)

Triglyceride Assay Kit (ab178780) is a sensitive, easy assay to measure triglyceride concentration in mammalian samples.
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Functional Studies - Triglyceride Assay Kit (Fluorometric, Reducing Samples) (AB178780)
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Functional Studies - Triglyceride Assay Kit (Fluorometric, Reducing Samples) (AB178780)

Example of triglyceride standard curve following assay protocol. Please note that this is an example curve and should not be used for experimental purposes.

Functional Studies - Triglyceride Assay Kit (Fluorometric, Reducing Samples) (AB178780)
  • FuncS

Supplier Data

Functional Studies - Triglyceride Assay Kit (Fluorometric, Reducing Samples) (AB178780)

Measurement of triglyceride levels in samples.

Mesurament of TG levels in rat liver (~15 μg), human serum (1 μL) and saliva (10 μL). Assays were performed according to kit protocol.

Schematic Diagram - Triglyceride Assay Kit (Fluorometric, Reducing Samples) (AB178780)
  • Schematic Diagram

Supplier Data

Schematic Diagram - Triglyceride Assay Kit (Fluorometric, Reducing Samples) (AB178780)

Representative image of Triglyceride Assay Kit (Fluorometric, Reducing Samples) ab178780

Components shown from left to right :

- PicoProbe I

- Substrate Mix I

- Cholesterol Esterase

- Triglyceride Standard

- Triglyceride Enzyme Mix

- Assay Buffer 5

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit.

Key facts

Detection method

Fluorescent

Sample types

Saliva, Heparin Plasma, Tissue, Suspension cells, Serum, Adherent cells

Results type

Quantitative

Sensitivity

< 0.4 µM

Assay time

1h

Assay Platform

Microplate reader

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Product details

Triglyceride Assay Kit (ab178780) is a sensitive, easy assay to measure triglyceride concentration in mammalian samples. In this triglyceride assay protocol, triglycerides are hydrolyzed to glycerol and fatty acid. The glycerol reacts with Triglyceride Enzyme Mix to form an intermediate product which in turn reacts with the probe and developer to generate fluorescence (Ex/Em 535/587 nm). The generated fluorescence is directly proportional to the amount of triglycerides.

Triglyceride Assay Kit (Fluorometric, Reducing Samples) ab178780 is suitable for measuring triglyceride levels in samples which contain reducing substances that may interfere with oxidase-based assays such as Triglyceride Assay Kit (ab65336).

Triglyceride assay protocol summary:
- add samples and standards to wells
- add lipase and incubate for 20 min
- add reaction mix and incubate for 30 min
- analyze with a microplate reader.

Please note: the general range is 0-10 nmol (colorimetric) and 0-1 nmol (fluorometric).

How other researchers have used Triglyceride Assay Kit ab178780
The Triglyceride assay kit has been used in publications in a variety of sample types, including:
- Human: Hepatocyte cell/cell line extract1,2
- Mouse: Plasma3, Skeletal muscle extract4, Serum5
References: 1-Liu Q et al. 2021, PMID: 34341705, 2-Hu LK et al. 2021, PMID: 34707711, 3-Panajatovic MV et al, PMID: 34066911, 4-Panajatovic MV et al, PMID: 34066911, 5-Maier JI et al. 2021, PMID: 34203913

Other Notes
This product was previously called K614 PicoProbe Triglyceride (TG) Fluorometric Assay Kit. Biovision was acquired by Abcam in 2021.

Triglycerides are the main constituent of vegetable oil, animal fat, LDL and VLDL, and play an important role as transporters of fatty acids as well as serving as an energy source. Triglycerides are broken down into fatty acids and glycerol, after which both can serve as substrates for energy producing and metabolic pathways. High blood levels of triglycerides are implicated in atherosclerosis, heart disease and stroke as well as in pancreatitis.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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What's included?

{ "values": { "100Test": { "sellingSize": "100 Test", "publicAssetCode":"ab178780-100Test", "assetComponentDetails": [ { "size":"1 x 300 µL", "name":"Triglyceride Standard", "number":"AB178780-CMP06", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Triglyceride Enzyme Mix", "number":"AB178780-CMP05", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Substrate Mix I", "number":"AB178780-CMP04", "productcode":"" }, { "size":"1 x 0.4 mL", "name":"PicoProbe I", "number":"AB178780-CMP02", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Cholesterol Esterase", "number":"AB178780-CMP01", "productcode":"" }, { "size":"1 x 25 mL", "name":"Assay Buffer 5", "number":"AB178780-CMP03", "productcode":"AB323940" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Triglycerides also known as triacylglycerols serve as the major form of fat storage in the body. Composed of three fatty acid chains attached to a glycerol backbone these molecules possess a molecular weight that varies depending on the fatty acid composition. Triglycerides are biosynthesized in liver cells and adipocytes. They accumulate in lipid droplets within these cells providing an energy reserve that organisms can access when necessary.
Biological function summary

Triglyceride molecules play an important role in energy metabolism and storage. Acting within the lipid storage complex these molecules ensure that energy derived from food is efficiently stored and made available for future energy demands. When the body requires energy an enzyme called lipase breaks down triglycerides into free fatty acids and glycerol which can then enter metabolic pathways to be used as cellular fuel.

Pathways

Triglycerides are integral to lipid metabolism and energy production. In lipid metabolism lipase converts triglycerides to free fatty acids and glycerol aiding in their mobilization across cellular membranes. These molecules integrate into the beta-oxidation pathway one of the important metabolic routes for energy production. Proteins such as hormone-sensitive lipase and adipose triglyceride lipase modulate their mobilization coordinating the release and utilization of energy reserves based on physiological needs.

Triglycerides have strong associations with hyperlipidemia and cardiovascular diseases. Elevated triglyceride levels in the body surpassing the normal triglyceride range increase the risk of such conditions. Hyperlipidemia characterized by high triglyceride or cholesterol levels often involves interactions with apolipoproteins which influence lipid transport and deposition. Cardiovascular diseases link to the buildup of lipoproteins of which triglycerides form a substantial part contributing to plaque formation in arterial walls.
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Product protocols

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Target data

See full target information Triglyceride
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Publications (20)

Recent publications for all applications. Explore the full list and refine your search

Mediators of inflammation 2025:9948679 PubMed40017524

2025

In Vitro, In Vivo, and In Silico Investigation of Synbiotic-Mediated Activation of PPAR- Curtails Nonalcoholic Steatohepatitis (NASH) in Wistar Rats by Inhibiting PNPLA3/SREBP1-c Lead Inflammatory Injury of Hepatic Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Dixa Sharma,Dhara Patel,Palash Mandal

Nature communications 14:8474 PubMed38123539

2023

Mtfp1 ablation enhances mitochondrial respiration and protects against hepatic steatosis.

Applications

Unspecified application

Species

Unspecified reactive species

Cecilia Patitucci,Juan Diego Hernández-Camacho,Elodie Vimont,Sonny Yde,Thomas Cokelaer,Thibault Chaze,Quentin Giai Gianetto,Mariette Matondo,Anastasia Gazi,Ivan Nemazanyy,David A Stroud,Daniella H Hock,Erminia Donnarumma,Timothy Wai

Molecular metabolism 79:101846 PubMed38030123

2023

Inhibiting the endoplasmic reticulum stress response enhances the effect of doxorubicin by altering the lipid metabolism of liver cancer cells.

Applications

Unspecified application

Species

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Maria Kopsida,Ada Lerma Clavero,Jaafar Khaled,David Balgoma,Clara Luna-Marco,Azazul Chowdhury,Sofi Sennefelt Nyman,Fredrik Rorsman,Charlotte Ebeling Barbier,Peter Bergsten,Hans Lennernäs,Mikael Hedeland,Femke Heindryckx

PloS one 18:e0283118 PubMed36930661

2023

The impact of hyperglycemia upon BeWo trophoblast cell metabolic function: A multi-OMICS and functional metabolic analysis.

Applications

Unspecified application

Species

Unspecified reactive species

Zachary J W Easton,Xian Luo,Liang Li,Timothy R H Regnault

Nutrients 15: PubMed36771214

2023

Validation of Knock-Out Caco-2 TC7 Cells as Models of Enterocytes of Patients with Familial Genetic Hypobetalipoproteinemias.

Applications

Unspecified application

Species

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Claire Bordat,Donato Vairo,Charlotte Cuerq,Charlotte Halimi,Franck Peiretti,Armelle Penhoat,Aurélie Vieille-Marchiset,Teresa Gonzalez,Marie-Caroline Michalski,Marion Nowicki,Noël Peretti,Emmanuelle Reboul

Frontiers in genetics 13:991875 PubMed36246583

2022

Aberrant lncRNA-mRNA expression profile and function networks during the adipogenesis of mesenchymal stem cells from patients with ankylosing spondylitis.

Applications

Unspecified application

Species

Unspecified reactive species

Shuizhong Cen,Mingxi Cai,Yihan Wang,Xiuyi Lu,Zhipeng Chen,Haobo Chen,Yingdong Fang,Changping Wu,Sujun Qiu,Zhenhua Liu

Journal of cellular and molecular medicine 26:4825-4836 PubMed35962606

2022

Dysregulated lipolysis and lipophagy in lipid droplets of macrophages from high fat diet-fed obese mice.

Applications

Unspecified application

Species

Unspecified reactive species

Yohannes Getiye,Tatiana Angel Rice,Brandon D Phillips,Daniel Fidel Carrillo,Guanglong He

Cellular and molecular life sciences : CMLS 79:354 PubMed35678878

2022

The protective effect of selenoprotein M on non-alcoholic fatty liver disease: the role of the AMPKα1-MFN2 pathway and Parkin mitophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Jingzeng Cai,Jiaqiang Huang,Jie Yang,Xiaoming Chen,Haoran Zhang,Yue Zhu,Qi Liu,Ziwei Zhang

Experimental and therapeutic medicine 22:1430 PubMed34707711

2021

MicroRNA-506-3p targets SIRT1 and suppresses AMPK pathway activation to promote hepatic steatosis.

Applications

Unspecified application

Species

Unspecified reactive species

Liang-Kai Hu,Jian-Qing Chen,Hao Zheng,Yuan-Ping Tao,Yuan Yang,Xuan-Fu Xu

Aging and disease 12:1238-1251 PubMed34341705

2021

Aged-related Function Disorder of Liver is Reversed after Exposing to Young Milieu Conversion of Hepatocyte Ploidy.

Applications

Unspecified application

Species

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Qinggui Liu,Fei Chen,Tao Yang,Jing Su,Shaohua Song,Zhi-Ren Fu,Yao Li,Yi-Ping Hu,Min-Jun Wang
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