Triglyceride Assay Kit (ab178780) is a sensitive, easy assay to measure triglyceride concentration in mammalian samples.
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- Nature communications 14:84742023Mtfp1 ablation enhances mitochondrial respiration and protects against hepatic steatosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCecilia Patitucci,Juan Diego Hernández-Camacho,Elodie Vimont,Sonny Yde,Thomas Cokelaer,Thibault Chaze,Quentin Giai Gianetto,Mariette Matondo,Anastasia Gazi,Ivan Nemazanyy,David A Stroud,Daniella H Hock,Erminia Donnarumma,Timothy WaiPubMed 38123539
- PloS one 18:e02831182023The impact of hyperglycemia upon BeWo trophoblast cell metabolic function: A multi-OMICS and functional metabolic analysis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZachary J W Easton,Xian Luo,Liang Li,Timothy R H RegnaultPubMed 36930661
- Nutrients 15:2023Validation of Knock-Out Caco-2 TC7 Cells as Models of Enterocytes of Patients with Familial Genetic Hypobetalipoproteinemias.Applications:Unspecified applicationReactive species:Unspecified reactive speciesClaire Bordat,Donato Vairo,Charlotte Cuerq,Charlotte Halimi,Franck Peiretti,Armelle Penhoat,Aurélie Vieille-Marchiset,Teresa Gonzalez,Marie-Caroline Michalski,Marion Nowicki,Noël Peretti,Emmanuelle ReboulPubMed 36771214
- Journal of cellular and molecular medicine 26:4825-48362022Dysregulated lipolysis and lipophagy in lipid droplets of macrophages from high fat diet-fed obese mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYohannes Getiye,Tatiana Angel Rice,Brandon D Phillips,Daniel Fidel Carrillo,Guanglong HePubMed 35962606
- Experimental and therapeutic medicine 22:14302021MicroRNA-506-3p targets SIRT1 and suppresses AMPK pathway activation to promote hepatic steatosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLiang-Kai Hu,Jian-Qing Chen,Hao Zheng,Yuan-Ping Tao,Yuan Yang,Xuan-Fu XuPubMed 34707711
- Aging and disease 12:1238-12512021Aged-related Function Disorder of Liver is Reversed after Exposing to Young Milieu Conversion of Hepatocyte Ploidy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQinggui Liu,Fei Chen,Tao Yang,Jing Su,Shaohua Song,Zhi-Ren Fu,Yao Li,Yi-Ping Hu,Min-Jun WangPubMed 34341705
- Cells 10:2021A Novel Model for Nephrotic Syndrome Reveals Associated Dysbiosis of the Gut Microbiome and Extramedullary Hematopoiesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJasmin I Maier,Manuel Rogg,Martin Helmstädter,Alena Sammarco,Gerd Walz,Martin Werner,Christoph SchellPubMed 34203913
- International journal of molecular sciences 22:2021Effects of Simvastatin on Lipid Metabolism in Wild-Type Mice and Mice with Muscle PGC-1α Overexpression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMiljenko V Panajatovic,Francois Singh,Stephan Krähenbühl,Jamal BouitbirPubMed 34066911
- Cells & development 166:2036782021Perinatal exposure to chlorpyrifos and/or a high-fat diet is associated with liver damage in male rat offspring.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMarion Guibourdenche,Hiba El Khayat El Sabbouri,Fidéline Bonnet,Narimane Djekkoun,Hafida Khorsi-Cauet,Aurélie Corona,Jean Guibourdenche,Véronique Bach,Pauline M Anton,Jérôme Gay-QuéheillardPubMed 33994353
- Journal of clinical and translational hepatology 8:359-3702020A 3D Human Liver Model of Nonalcoholic Steatohepatitis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMarion Duriez,Agnes Jacquet,Lucile Hoet,Sandrine Roche,Marie-Dominique Bock,Corinne Rocher,Gilles Haussy,Xavier Vigé,Zsolt Bocskei,Tamara Slavnic,Valérie Martin,Jean-Claude Guillemot,Michel Didier,Aimo Kannt,Cécile Orsini,Vincent Mikol,Anne-Céline Le FèvrePubMed 33447518
Key facts
- Detection method
- Fluorescent
- Sample types
- Saliva, Heparin Plasma, Tissue, Suspension cells, Serum, Adherent cells
- Assay type
- Quantitative
- Reactive species
- Mammals
- Assay time
- 1h
- Sensitivity
- < 0.4 µM
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Triglyceride Assay Kit (ab178780) is a sensitive, easy assay to measure triglyceride concentration in mammalian samples.
Key facts
- Detection method
- Fluorescent
- Sample types
- Saliva, Heparin Plasma, Tissue, Suspension cells, Serum, Adherent cells
- Assay type
- Quantitative
- Reactive species
- Mammals
- Assay time
- 1h
- Assay Platform
- Microplate reader
- Sensitivity
- < 0.4 µM
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
Triglyceride Assay Kit (ab178780) is a sensitive, easy assay to measure triglyceride concentration in mammalian samples. In this triglyceride assay protocol, triglycerides are hydrolyzed to glycerol and fatty acid. The glycerol reacts with Triglyceride Enzyme Mix to form an intermediate product which in turn reacts with the probe and developer to generate fluorescence (Ex/Em 535/587 nm). The generated fluorescence is directly proportional to the amount of triglycerides.
Triglyceride Assay Kit (Fluorometric, Reducing Samples) ab178780 is suitable for measuring triglyceride levels in samples which contain reducing substances that may interfere with oxidase-based assays such as Triglyceride Assay Kit (Triglyceride Assay Kit - Quantification ab65336).
Triglyceride assay protocol summary:
- add samples and standards to wells
- add lipase and incubate for 20 min
- add reaction mix and incubate for 30 min
- analyze with a microplate reader.
Please note: the general range is 0-10 nmol (colorimetric) and 0-1 nmol (fluorometric).
How other researchers have used Triglyceride Assay Kit ab178780
The Triglyceride assay kit has been used in publications in a variety of sample types, including:
- Human: Hepatocyte cell/cell line extract1,2
- Mouse: Plasma3, Skeletal muscle extract4, Serum5
References: 1-Liu Q et al. 2021, PMID: 34341705, 2-Hu LK et al. 2021, PMID: 34707711, 3-Panajatovic MV et al, PMID: 34066911, 4-Panajatovic MV et al, PMID: 34066911, 5-Maier JI et al. 2021, PMID: 34203913
Other Notes
This product was previously called K614 PicoProbe Triglyceride (TG) Fluorometric Assay Kit. Biovision was acquired by Abcam in 2021.
Triglycerides are the main constituent of vegetable oil, animal fat, LDL and VLDL, and play an important role as transporters of fatty acids as well as serving as an energy source. Triglycerides are broken down into fatty acids and glycerol, after which both can serve as substrates for energy producing and metabolic pathways. High blood levels of triglycerides are implicated in atherosclerosis, heart disease and stroke as well as in pancreatitis.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Triglycerides also known as triacylglycerols serve as the major form of fat storage in the body. Composed of three fatty acid chains attached to a glycerol backbone these molecules possess a molecular weight that varies depending on the fatty acid composition. Triglycerides are biosynthesized in liver cells and adipocytes. They accumulate in lipid droplets within these cells providing an energy reserve that organisms can access when necessary.
- Biological function summary
Triglyceride molecules play an important role in energy metabolism and storage. Acting within the lipid storage complex these molecules ensure that energy derived from food is efficiently stored and made available for future energy demands. When the body requires energy an enzyme called lipase breaks down triglycerides into free fatty acids and glycerol which can then enter metabolic pathways to be used as cellular fuel.
- Pathways
Triglycerides are integral to lipid metabolism and energy production. In lipid metabolism lipase converts triglycerides to free fatty acids and glycerol aiding in their mobilization across cellular membranes. These molecules integrate into the beta-oxidation pathway one of the important metabolic routes for energy production. Proteins such as hormone-sensitive lipase and adipose triglyceride lipase modulate their mobilization coordinating the release and utilization of energy reserves based on physiological needs.
- Associated diseases and disorders
Triglycerides have strong associations with hyperlipidemia and cardiovascular diseases. Elevated triglyceride levels in the body surpassing the normal triglyceride range increase the risk of such conditions. Hyperlipidemia characterized by high triglyceride or cholesterol levels often involves interactions with apolipoproteins which influence lipid transport and deposition. Cardiovascular diseases link to the buildup of lipoproteins of which triglycerides form a substantial part contributing to plaque formation in arterial walls.
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2 product images
Functional Studies - Triglyceride Assay Kit (Fluorometric, Reducing Samples) (ab178780)
Example of triglyceride standard curve following assay protocol. Please note that this is an example curve and should not be used for experimental purposes.
Functional Studies - Triglyceride Assay Kit (Fluorometric, Reducing Samples) (ab178780)
Measurement of triglyceride levels in samples.
Mesurament of TG levels in rat liver (~15 μg), human serum (1 μL) and saliva (10 μL). Assays were performed according to kit protocol.
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