Triglyceride Assay Kit - Quantification

Triglyceride Assay Kit (ab65336) is a sensitive, easy assay to measure triglyceride concentration in mammalian samples. In the triglyceride assay protocol, triglycerides are converted to free fatty acids and glycerol. Glycerol is then oxidized to generate a product which reacts with a probe to generate color (spectrophotometry at λ= 570 nm) and fluorescence (Ex/Em = 535/587 nm).

Triglyceride assay protocol summary:
- add samples and standards to wells
- add assay buffer and lipase, and incubate for 20 min
- add triglyceride reaction mix and incubate for 60 min
- analyze with microplate reader

Please note: the general range is 0-10 nmol (colorimetric) and 0-1 nmol (fluorometric).

If your sample contains reducing substances, they are likely to interfere with the assay. In this case, we recommend using Triglyceride Assay Kit (Fluorometric, Reducing samples) Triglyceride Assay Kit (Fluorometric, Reducing Samples) ab178780.

Target background

Triglycerides are the main constituent of vegetable oil, animal fat, LDL and VLDL, and play an important role as transporters of fatty acids as well as serving as an energy source. Triglycerides are broken down into fatty acids and glycerol, after which both can serve as substrates for energy producing and metabolic pathways. High blood levels of triglycerides are implicated in atherosclerosis, heart disease and stroke as well as in pancreatitis.

Review our Metabolism Assay Guide to learn about assays for metabolites, metabolic enzymes, mitochondrial function, and oxidative stress, and also about how to assay metabolic function in live cells using your plate reader.

Technical Note: Triglyceride is unlikely to be detectable in healthy urine but may be elevated in the presence of infection or disease of the urinary tract.

This product is manufactured by BioVision, an Abcam company and was previously called K622 Triglyceride Quantification Colorimetric/Fluorometric Kit. K622-100 is the same size as the 100 test size of ab65336.

How other researchers have used Triglyceride Assay Kit ab65336

The Triglyceride assay kit has been used in publications in a variety of sample types, including:
- Human: serum¹, plasma², mammary epithelial and mammary cancer cell line lysate³, Huh7.5 hepatocyte-derived cell line lysate⁴, primary liver cell line lysates⁵, sebocyte cell culture lysates⁶
- Mouse: hepatocyte cell lysates⁷, liver extract⁸, serum⁹, plasma¹⁰, kidney extracts¹¹, liver tissue and serum¹², cardiac tissue extracts¹³
- Rat: liver tissue extract¹⁴, plasma¹⁵
- Drosophila¹⁶

^{References: 1-Huang Y et al. 2019, PMID: 30778327, 2-Wilson et al 2018; PMID: 29534545, 3-Yen MC et al. 2019, PMID: 30911270, 4-Kim D et al. 2018, PMID: 30127285, 5-Boteon Y et al. 2018, PMID: 30044872, 6- href=Jin S and Lee MY 2018, PMID: 30400322, 7-Zhang W et al. 2019, PMID: 30664220, 8-Brial F et al. 2019, PMID: 30842494, 9-Liu J et al. 2018, PMID: 30344653 and Patton A et al. 2018, PMID: 29666152, 10-Fernandes et al. 2018, PMID: 29320745 and Körholz JC et al 2018, PMID: 30362941 and Liang et al. 2018, PMID: 29659562, 11-Ding W et al. 2018, PMID: 29563333, 12-Cui XB et al. 2018, PMID: 29449334, 13-Rohm M et al. 2018<. PMID: 29610263, 14-Yu S et al. 2018, PMID: 29867579, 15-García-Ruiz et al. 2018, PMID: 29367725, 16-Wen CA and Ballard JWO 2019, PMID: 30805163}