Triglyceride Assay Kit - Quantification

• Biochemical assay

Lab

Biochemical assay - Triglyceride Assay Kit - Quantification (AB65336)

Diagram showing the principles of the Triglyceride assay method.

• Biochemical assay

PubMed

Biochemical assay - Triglyceride Assay Kit - Quantification (AB65336)

Triglyceride assay kit ab65336 and free fatty acid assay kit ab65341 used with heart tissue.

Han et al. used Triglyceride assay kit ab65336 and Free Fatty Acid assay kit ab65341 to investigate cardiac abnormalities in high fat diet fed mice when researching the role of the long non-coding RNA Lipid-Droplet Transporter (LIPTER).

The concentration of free fatty acids (FA) (f, n = 5 mice each group) were quantified using the Free Fatty Acid Assay kit (ab65341), and triglyceride (TG) concentrations (g, n = 7 mice each group) were quantified using the Triglyceride Quantification Assay Kit (ab65336).

Approximately 25 mg of mouse heart tissue per heart was collected and homogenized in 500 μl lipid extraction buffer (ab211044) using a Beadbug Homogenizer. After centrifugation, the supernatant was removed to a new tube. FA or TAG concentration was measured according to the manufacturer’s protocols. The FA and TAG concentrations in WT and genetically modified mouse hearts were normalized to the collected heart tissue weights.

• FuncS

PubMed

Functional Studies - Triglyceride Assay Kit - Quantification (AB65336)

Hepatic triglyceride levels was measured using ab65336 in male and female wild-type (WT) or AT₂KO (knockout) mice with either normal diet (ND) or high fat diet (HFD).

Image from Samuel P et al., PLoS One 8(1), Fig 7b. doi: 10.1371/journal.pone.0048425. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

• FuncS

Lab

Functional Studies - Triglyceride Assay Kit - Quantification (AB65336)

Fluorometric triglyceride standard curve : mean of duplicates (+/- SD) with background reads subtracted

• FuncS

Supplier Data

Functional Studies - Triglyceride Assay Kit - Quantification (AB65336)

Colorimetric triglyceride standard curve using ab65336.

• FuncS

Lab

Functional Studies - Triglyceride Assay Kit - Quantification (AB65336)

Triglyceride measured in cell culture lysates showing quantity (nmol) per 1 mln cells.

Samples with the concentration of 1e7 cells/mL were used. Samples were diluted 40-80 fold and measured fluorometrically.

HepG2 cells were treated with 25 uM Chloroquine for 72h.

• Biochemical assay

PubMed

Biochemical assay - Triglyceride Assay Kit - Quantification (AB65336)

Triglyceride assay kit ab65336 and cholesterol assay kit ab65359 used with HepG2 cell lysates.

Pozzi et al. used used the triglyceride assay kit and cholesterol assay kit to investigate the effect of antipsychotics on the SREBP pathway and on AMPK activation.

HepG2 cells serum-starved overnight and pre-treated with DMEM low glucose 20% FBS for 3 h, were incubated with 5 μM U18666A, 25 μM risperidone (RIS), ziprasidone (ZIP) or olanzapine (OLA), the SREBP2 positive control lovastatin 25 μM (LOV), and the SREBP1 positive control T0901317 10 μM (T09) for 24 h.

Triglycerides (TG) were extracted and quantified from HepG2 cells treated with the indicated compounds using Abcam ab65336. Briefly, 5 x 10^6 cells were harvested and lysed in 5% v/v NP-40, then samples were repeatedly heated at 98°C to solubilise triglycerides; a sample was taken for BCA protein quantification as normaliser, and the indicated amount of sample was processed with lipase and reaction reagents. Fluorescence emitted by the chromogen dye was quantified on Fluoroskan microplate reader (Ascent FL, Thermo Fisher Scientific) at 544/590 nm. Triglyceride fluorescence was reported as fold increase over levels of untreated cells (one way ANOVA followed by Dunnett's multiple comparison test, n > 3 experiments; *vs. unt cells).

Total cholesterol was extracted from HepG2 cells treated with the indicated compounds by using cholesterol assay kit ab65359. Briefly, 10^6 cells were harvested and lysed in 100 mM NaCl, 10 mM TRIS pH 7.4, 1 mM EGTA, 2 mM MgCl2 and 1% v/v Triton-X100; samples were repeatedly vortexed and chilled on ice to solubilise cholesterol; a sample was taken for BCA protein quantification as normaliser, while the rest was mixed with isopropanol-chloroform 11 : 7 and centrifuged to separate the organic phase containing cholesterol. The organic phase was taken and evaporated, cholesterol was then resuspended and processed with cholesterol esterase and reaction reagents. Fluorescence emitted by the chromogen dye was quantified on Fluoroskan microplate reader at 544/590 nm. Cholesterol fluorescence was reported as fold increase over levels of untreated cells (one way ANOVA followed by Dunnett's multiple comparison test, n > 3 experiments; *vs. unt cells).

• Biochemical assay

PubMed

Biochemical assay - Triglyceride Assay Kit - Quantification (AB65336)

Triglyceride assay kit ab65336 and free fatty acid assay kit ab65341 used with liver tissue and plasma samples respectively.

Samuel et al. used the triglyceride assay kit and free fatty acid assay kit to understand gender differences in metabolic syndrome disorders in angiotensin receptor AT2R knockout mice.

Plasma free fatty acid levels were measured by colorimetric method using the Abcam free fatty acid quantification kit.

Hepatic triglyceride levels was measured using ab65336 in male and female wild-type (WT) or AT2KO (knockout) mice with either normal diet (ND) or high fat diet (HFD). For hepatic triglyceride measurement, livers were dissected and snap-frozen in liquid nitrogen and stored at −80°C. In brief, lipids were extracted by homogenizing 25 mg liver tissue in 1 ml 5% Triton-X100 in water, then slowly heated to 80°C in water bath for 5 min. The samples were cooled down and again heated to solubilize all triglycerides into solution. The samples were centrifuged for 5 min and supernatants were diluted 10 fold with dH2O for quantification.

• Biochemical assay

PubMed

Biochemical assay - Triglyceride Assay Kit - Quantification (AB65336)

Triglyceride assay kit ab65336 and cholesterol assay kit ab65390 used with mouse serum.

Zuo et al. used Triglyceride assay kit ab65336 and Cholesterol assay kit ab65390 to investigate the impact of hepatocyte-specific AAV-mediated knockout of ANGPTL3 in mice.

Measurements of serum triglyceride (TG) and total cholesterol (TC) in mice at 1–4 weeks after AAV9-hAAT-Anc/Angptl3 administration. The control (Ctrl) mice received no treatments.

Blood samples collected from the animals were allowed for clotting at room temperature for 10 min. Serum was isolated by centrifugation at 5000 rpm for 10 min and stored at -80 °C in small aliquots. Determination of TG and TC was performed using the triglyceride and cholesterol assay kit (Abcam, ab65336 and ab65390, respectively) according to the manufacture’s instruction.

• Biochemical assay

PubMed

Biochemical assay - Triglyceride Assay Kit - Quantification (AB65336)

Triglyceride assay kit ab65336 and Free Fatty Acid assay kit ab65341 used with mouse liver tissue.

Yue et al. used triglyceride assay kit ab65336 and free fatty acid assay kit ab65341 to investigate the protective effects of IL-22 on alleviating alcoholic hepatitis.

Administration of IL-22 reduces alcohol-induced lipid accumulation in mouse liver. PF (pair fed), AF (alcohol fed), AF+IL-22 (alcohol fed with IL-22 treatment).

(A) BODIPY 493/503 staining of mouse liver. Neutral lipids were stained in green, and nuclei were counterstained in blue. (B) Hepatic triglyceride (TG) and free fatty acid (FFA) levels.

Quantification assays of triglycerides (TG) and free fatty acids (FFA) in the liver were conducted using commercial kits from Abcam (Waltham, MA; ab65336 and ab65341) per the manufacturer’s instructions.

• Biochemical assay

PubMed

Biochemical assay - Triglyceride Assay Kit - Quantification (AB65336)

Triglyceride assay kit ab65336, Cholesterol assay kit ab65390, and Free Fatty Acid assay kit ab65341 used with rat serum and rat liver.

Li et al. used Triglyceride assay kit ab65336, Cholesterol assay kit ab65359, and Free Fatty Acid assay kit ab65341 to investigate the impact of using L. reuteri treatment to ameliorate the impact of biorhythm disorder-ignited dyslipidemia in long-term darkness treated rats.

The concentrations of triglycerides (TG), total cholesterol (CHOL), HDL-cholesterol (HDL-C), and free fatty acids (NEFA) in rat sera and liver tissues were detected using Triglyceride Quantification Assay Kit (ab65336), HDL and LDL/VLDL Cholesterol Assay Kit (ab65390). and Free Fatty Acid Quantification Assay Kit (ab65341), respectively. All procedures were carried out following the recommended instructions provided by the kit manufacturers.

• Other

Supplier Data

Other - Triglyceride Assay Kit - Quantification (AB65336)

Representative image of Triglyceride Assay Kit - Quantification ab65336

Components shown from left to right :

- OxiRed Probe

- Cholesterol Esterase

- Triglyceride Standard

- Enzyme Mix VI

- Assay Buffer 5

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit.